1 | 10/4/2019 | Leica 10x/0.25, LPM Thorlabs LPM4 and TP2,X= 9216.5 / y=26735.2 / z=10000, measured at 100% | |||||||||||||||||
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2 | nominal power at laser head | control powerMeter | all values measured in mW | ||||||||||||||||
3 | installation minimum (70% of installation reference) | ||||||||||||||||||
4 | critical value (50% of installation reference) | ||||||||||||||||||
5 | date | time | temperature ambient/microscope | running hours | Homing done? DONT DO IT!!!!! | Total Hours (SIM lasers) | Total Hours (TIRF lasers) | Laser % | No laser on | 405-SIM | 488-SIM | 568_SIM | 640-SIM | 405-TIRF | 488-TIRF | 568-TIRF | 640-TIRF | UFIOS diode 785nm | comments |
6 | 7/5/2019 | 4:00 PM | No | 100 | 0 | 6.26 | 6.72 | 8.7 | 13.3 | 19.4 | 28.5 | 32.7 | 43 | Laure | |||||
7 | 8/1/2019 | 1:59 PM | 0.3 | No | 173 | 100 | 0 | 11 | 6.7 | 7.7 | 13.5 | not yet | Laure & Hifsa, Flip mirror stuck on SIM side: not possible to measure conv. mode. No protocol yet for UFIOS. | ||||||
8 | 8/6/2019 | 10:14 AM | 21.7 | 0.5 | No | 179 | 100 | 0 | 9.87 | 6.2 | 7.1 | 12.94 | not yet | Hifsa - not possible to measure conv. mode. No protocol yet for UFIOS. | |||||
9 | 8/20/2019 | 12:02 PM | 264 | 100 | 0 | 10.55 | 6.63 | 7.02 | 12.64 | 19.22 | 27.7 | 23.6 | 43.4 | not yet | Laure & Hifsa, UFIOS not measured as there are issues with the electronics board. | ||||
10 | 8/30/2019 | 3:15 PM | 21.9 | 0.5 | No | 207 | 100 | 0 | 10.89 | 6.42 | 7.22 | 12.76 | 19.32 | 27.29 | 27.1 | 42.8 | not yet | Sebastian & Hifsa, UFIOS not measured as there are issues with the electronics board. | |
11 | 9/3/2019 | 10:37 AM | 20.7 | 0.5 | No | 207 | 100 | 0 | 10.54 | 6.56 | 7.97 | 12.71 | 19.17 | 27.15 | 27.8 | 42.9 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
12 | 9/10/2019 | 11:23 AM | 20.8 | 0.5 | No | 210 | 100 | 0 | 12.18 | 8.84 | 6.87 | 12.15 | 17.91 | 25.46 | 25.2 | 40.1 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
13 | 9/17/2019 | 11:19 AM | 20.4 | 0.5 | No | 280 | 100 | 0 | 13.71 | 10.53 | 7.73 | 12.94 | 18.37 | 26.63 | 25.3 | 41.6 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
14 | 9/26/2019 | 10:36 AM | 20.1 | 0.5 | No | 290 | 100 | 0 | 12.98 | 9.02 | 7.26 | 12.63 | 19.16 | 27.66 | 24.6 | 42.7 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
15 | 10/4/2019 | 3:32 PM | 20.5 | 1 | No | 297 | 100 | 0 | 9.47 | 5.93 | 7.42 | 12.63 | 17.5 | 25.37 | 26.3 | 39.2 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. ( System crashed, performed a complete hardware restart with Dan) | |
16 | 10/8/2019 | 2:20 PM | 20.1 | 0.5 | No | 298 | 100 | 0 | 9.41 | 7.2 | 7.88 | 12.5 | 17.26 | 26.36 | 24.5 | 39.3 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
17 | 10/17/2019 | 10:07 AM | 19.7 | 0.5 | No | 231 | 100 | 0 | 12.48 | 7.1 | 6.97 | 12.2 | 17.48 | 26.3 | 24.3 | 38.8 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
18 | 10/25/2019 | 15:30 PM | 20.1 | 0.5 | Yes | - | 100 | 0 | 9.19 | 5.84 | 7.32 | 12.32 | 16.4 | 25.3 | 23.9 | 37.5 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board. | |
19 | 11/15/2019 | 12:15 PM | 21.6 | 0.5 | Yes | 248 | 100 | 0 | 10.07 | 6.17 | 7.87 | 12.55 | 17.91 | 29.6 | 25.3 | 38.7 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). | |
20 | 11/20/2019 | 12:42 PM | 21.3 | 0.5 | Yes | 248 | 100 | 0 | 10.1 | 6.65 | 7.54 | 12.48 | 17.96 | 28.9 | 25.64 | 38.14 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). | |
21 | 11/27/2019 | 12:00 PM | 20.7 | 0.5 | Yes | - | 100 | 0 | 10.5 | 6.3 | 7.8 | 12.5 | 17.68 | 29.1 | 25.3 | 38.9 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). | |
22 | 12/6/2019 | 5:30 PM | 20.1 | 0.5 | Yes | - | 100 | 0 | 12.4 | 7.9 | 7.95 | 12.1 | 16.05 | 22.3 | 21.99 | 31.02 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). measured with TP1 | |
23 | 12/11/2019 | 11:34 AM | 20.3 | 0.5 | Yes | 265 | 100 | 0 | 13.56 | 8.08 | 8.47 | 12.32 | 14.06 | 22.63 | 21.46 | 30.28 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). measured with TP1 | |
24 | 12/19/2019 | 3:34 PM | 21.3 | 0.5 | - | 290 | 100 | 0 | 10.36 | 5.7 | 7.11 | 11.6 | 17.5 | 27.3 | 21.8 | 34.7 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). measured with TP3 | |
25 | 1/3/2020 | 2:45 PM | 21.4 | 0.5 | Yes | - | 100 | 0 | 10.58 | 6.3 | 7.25 | 12.36 | 18.02 | 28.14 | 22.3 | 25.08 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board (Google Drive at system unresponsive, LPM measurements were added manually using another computer). measured with TP3 | |
26 | 1/16/2020 | 11:19 AM | 20.4 | 0.5 | Yes | 628 | 100 | 0 | 10.27 | 5.82 | 7.34 | 11.5 | 17.7 | 27.7 | 24.4 | 34.5 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
27 | 1/23/2020 | 11:52 AM | 20.3 | 0.5 | Yes | 629 | 100 | 0 | 9.8 | 18.6 | 17.8 | 11.3 | 17.8 | 27.5 | 23.4 | 34.8 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
28 | 1/30/2020 | 12:01 PM | 21.3 | 0.5 | Yes | 637 | 100 | 0 | 10.15 | 5.82 | 6.82 | 11.7 | 17.7 | 24.6 | 23.5 | 37.6 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
29 | 2/6/2020 | 12:16 PM | 20.7 | 0.75 | yes | 642 | 100 | 0 | 10.25 | 5.79 | 6.93 | 11.26 | 18.1 | 25.3 | 23.1 | 37.84 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
30 | 2/13/2020 | 11:01 AM | 20.1 | 0.5 | yes | 644 | 100 | 0 | 10.31 | 5.81 | 6.96 | 11.27 | 18.3 | 24.9 | 23.3 | 34.4 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
31 | 2/20/2020 | 12:23 PM | 21.3 | 0.5 | yes | 345 | 100 | 0 | 10.32 | 5.83 | 7.04 | 11.8 | 17.5 | 25.3 | 22.8 | 34.5 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
32 | 2/27/2020 | 1:37 PM | 21.7 | 0.5 | yes | 645 | 100 | 0 | 10.24 | 5.44 | 6.94 | 11.7 | - | - | - | - | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
33 | 3/12/2020 | 11:22 AM | 21.4 | 0.5 | yes | 647 | 100 | 0 | 10.16 | 5.6 | 5.63 | 11 | 17.6 | 27.1 | 16.4 | 34.2 | not yet | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP4 | |
34 | Corona virus shutdown | ||||||||||||||||||
35 | 5/27/2020 | 12:00 AM | 21.1 | 1 | yes | 649 | 100 | 12.4 | 8.7 | 6.3 | 11 | 6.8 | 3 | 78 | 62.5 | Laure, TP3, LPM4, after laser homing | |||
36 | 12.5 | 8.8 | 6.1 | 10.8 | 17.3 | 26.8 | 20.3 | 35.5 | Laure, TP3, LPM4, after laser homing | ||||||||||
37 | 6/19/2020 | 2:45 PM | 20.9 | 0.5 | yes | 652 | 100 | 0 | 9.01 | 12.74 | 10.43 | 16.7 | - | - | - | - | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
38 | 6/26/2020 | 3:55 PM | 21.3 | 0.5 | yes | 652 | 100 | 0 | 12.35 | 8.59 | 5.32 | 17.4 | - | - | - | - | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
39 | 7/3/2020 | 12:54 PM | 20.5 | 0.5 | yes | 100 | 0 | 12.3 | 8.47 | 5.86 | 16.98 | - | - | - | - | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | ||
40 | 9/18/2020 | 6:58 PM | 22.3 | 2 | yes | 670 | 100 | 0 | 11.87 | 9.09 | 5.71 | 11.4 | 17.2 | 26.3 | 19.5 | 34.89 | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
41 | 10/8/2020 | 3:47 PM | 21.3 | 0.5 | YES | 372 | 100 | 0 | 11.81 | 9.06 | 6.22 | 11.7 | 17.3 | 26.4 | 18.1 | 34.9 | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
42 | 11/6/2020 | 12:16 PM | 21.2 | 0.5 | yes | 674 | 100 | 0 | 4.8 | 5.43 | 6.03 | 10.5 | 15.36 | 25.1 | 18 | 32.8 | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | ||
43 | 11/10/2020 | 4:18 PM | 20.7 | 0.5 | yes | 674 | 100 | 0 | 10.9 | 6.3 | 6.12 | 11.17 | 15.4 | 24.9 | 19.05 | 33.63 | - | Hifsa, UFIOS not measured as there are issues with the electronics board - measured with TP3 | |
44 | 12/11/2020 | 2:22 PM | 0.5 | yes | 727 | 100 | 0 | 11.45 | 8.73 | 5.52 | 11.71 | - | - | - | - | - | Hifsa and Catarina, UFIOS not measured as there are issues with the electronics board - measured with TP2 | ||
45 | 8/2/2021 | 2:11 PM | 21.5/25.5 | 1 | yes | 743 | 100 | 0 | 10.7 | 9.59 | 7.89 | 10.5 | - | - | - | - | - | Catarina, UFIOS not measured, TIRF not measured, 568 giving invalid response on status - measured with TP3, LPM5 | |
46 | 15/03/2021 | 4:00 PM | 22.5/26 | 2 | yes | 757 | 100 | 11.63 | 9.2 | 5.6 | 11.1 | 17 | 27.3 | 16.6 | 36.3 | - | Catarina and Britta, UFIOS not measured, lasers homed, Camera alignment performed - measured with TP3, LPM5 | ||
47 | 19/04/2021 | 3:30 PM | 23.5/25 | 1 | no | 100 | 11.3 | 8.6 | 5.1 | 11.3 | 17.4 | 27.3 | 15.1 | 36.6 | - | Catarina, UFIO not measured, measured for 3D-SIM and TIRF, lasers failed to home, a fuse blew & power cycled the system - measured with TP3, LPM5 | |||
48 | 5/17/2021 | 11:05 AM | 23.5/25.3 | 0.5 | yes | 460 | 100 | 11.4 | 9.4 | 4.4 | 11.2 | 17.3 | 27 | 11.3 | 36.2 | - | Catarina & Britta, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | ||
49 | 31/05/2021 | 2:30 PM | 24/25 | 0.5 | yes | 463 | 100 | 0 | 8.01 | 18 | 10 | 12.1 | 17.3 | 27 | 13 | 36.5 | - | Catarina, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
50 | 6/21/2021 | 4:37 PM | 24/25.5 | 0.5 | yes | 464 | 100 | 0 | 7.97 | 18 | 8.2 | 12.1 | 17.2 | 26.6 | 9.2 | 36.3 | - | Catarina, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
51 | 7/26/2021 | 3:01 PM | 23.5/25.5 | 1 | yes | 465 | 100 | 0 | 7.81 | 17.8 | 9.4 | 12 | 17.1 | 26.4 | 10.9 | 36.4 | - | Catarina, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
52 | 8/12/2021 | 11:40 AM | 23.5/25 | 1 | yes | 772 | 100 | 0 | 8.7 | 5.5 | 4.7 | 11 | 17.1 | 26.4 | 13.1 | 36 | - | Catarina, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
53 | 9/10/2021 | 10:51 AM | 23.5/25.5 | 1 | yes | 777 | 478 | 100 | 0.03 | 8.7 | 5.58 | 4.5 | 11 | 17.1 | 26.5 | 12 | 36 | - | Catarina & Jen, UFIO not measured, lasers did not respond to homing, measured for 3D-SIM and TIRF, Camera alignment performed - measured with TP3, LPM5 |
54 | 2021/09/13 POWER CUT IN DRESDEN | ||||||||||||||||||
55 | 9/14/2021 | 11:14 AM | 22.5/24.5 | 0.5 | yes | 777 | 479 | 100 | 0 | 7.78 | 17.6 | 10.3 | 12.1 | 17.1 | 26.2 | 12.2 | 35.9 | - | Catarina, UFIO not measured, lasers would not switch ON intially, measured for 3D-SIM and TIRF - measured with TP3, LPM5 |
56 | 10/6/2021 | 12:00 AM | 23.8/24.8 | 0.5 | yes | 777 | 479 | 100 | 7.7 | 17.8 | 8.7 | 12.2 | 17.1 | 26.5 | 10.4 | 36.3 | - | Catarina & YH, UFIO not measured, lasers would not switch ON intially, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
57 | 10/18/2021 | 5:02 PM | 22.8/25 | 0.5 | yes | 780 | 482 | 100 | 11.5 | 14.1 | 5.5 | 11.3 | 17.1 | 26.6 | 12.3 | 36.2 | - | Catarina & YH, UFIO not measured, measured for 3D-SIM and TIRF - measured with TP3, LPM5 | |
58 | 2021/11/02 SERVICE VISIT FROM LEICA | ||||||||||||||||||
59 | 11/15/2021 | 2:16 PM | 23.5/25.5 | 1 | yes | 792 | 494 | 100 | 0 | 9.2 | 2.5 | 5.4 | 11.7 | 16.7 | 26.7 | 15.2 | 35.9 | - | Catarina & YH UFIO not measured, measured for 3D-SIM and TIRF, strange values for the SIM lasers!!! - measured with TP3, LPM5 |
60 | 11/29/2021 | 3:02 PM | 21.5/24.5 | 1 | yes | 793 | 495 | 100 | 0 | 12.17 | 3.01 | 5.4 | 11.1 | 16.8 | 27 | 14.8 | 35.9 | - | Catarina & YH, software slow again, maybe communication with LCM, replaced batteries for lamp in inclosure, UFIO working - measured with TP3, LPM5 |
61 | 2021/12/07-09 VISIT FROM LEICA FOR ENVIRONMENTAL CONTROL | ||||||||||||||||||
62 | 20211222: Homing the lasers: Please follow the advice from the supplier: Do NOT use the home function unless specifically advised by a service engineer to do so. Regular homing is not necessary and can lead to misalignments if performed, for example, when the lasers are not fully warmed up. | ||||||||||||||||||
63 | 12/21/2021 | 4:04 PM | 1 | No | - | - | 100 | 0 | 9.19 | 5.58 | 5.04 | 11.6 | 16.8 | 27.1 | 15.4 | 35.7 | - | YH software slow again, measured with TP3, LPM5 | |
64 | 1/24/2022 | 4:08 PM | 22.5/25 | 0.5 | No | - | - | 100 | 0 | 8.26 | 17.8 | 11.8 | 11.9 | 16.64 | 26.7 | 14 | 35.8 | - | YH & Catarina software slow again, HW not switching ON, had to power cycle the entire system 3x - measured with TP3, LPM5 |
65 | 2/9/2022 | 2:42 PM | 23/25 | 0.5 | No | 801 | 506 | 100 | 0 | 8.03 | 17.7 | 11.5 | 11.2 | 16.63 | 26.7 | 11.3 | 35.7 | - | Catarina - measured with TP3, LPM5 |
66 | 2022/02/17-18 VISIT FROM LEICA FOR UFIO | ||||||||||||||||||
67 | 2/21/2022 | 4:15 PM | 23.5/25.5 | 1 | No | 810 | 519 | 100 | 0 | 11.81 | 6.58 | 4.94 | 11.2 | 16.75 | 27.1 | 15.1 | 36 | - | Catarina, UFIO not working - measured with TP3, LPM5 |
68 | 4/22/2022 | 11:37 AM | 23.5/25.5 | 0.5 | No | 822 | 524 | 100 | 0 | 9.3 | 5.6 | 4 | 11.1 | 16.6 | 26.7 | 11.4 | 35.8 | - | Catarina - measured with TP3, LPM5 |
69 | 5/12/2022 | 12:32 PM | 22.5/25 | 1 | No | 827 | 526 | 100 | 0 | 9.8 | 5.5 | 4.3 | 11.1 | 16.7 | 26.5 | 12 | 35.7 | - | Catarina - measured with TP3, LPM5 |
70 | 5/23/2022 | 11:31 AM | 24/26 | 0.5 | No | 829 | 528 | 100 | 0 | 11.75 | 7.03 | 4 | 11.5 | 16.55 | 26.5 | 10.3 | 35.8 | - | Catarina - measured with TP3, LPM4 |
71 | 6/20/2022 | 11:32 AM | 23/25 | 1.5 | No | 843 | 534 | 100 | 0 | 8.1 | 5.52 | 4.24 | 11 | 16.49 | 26.3 | 12.3 | 35.7 | Catarina & Catrin, lasers still fluctuating a lot - measured with TP3, LPM4 | |
72 | 8/15/2022 | 4:36 PM | 23.7/25 | 5 | No | 864 | 544 | 100 | 0 | 9.07 | 5.52 | 4.8 | 11.2 | 16.38 | 23.18 | 12.82 | 35.78 | - | Catarina & Britta, lasers still fluctuating a lot - measured with TP3, LPM5 |