PI that you are collaborating with

Sample type (targeted parameters and methodology, use the space here to explain, or send Ivona an email - see below)

Additional data needed (if yes, write in what kind, if not write no)

If sample requires additional water collection, please estimate volume and frequency (time and depth)?

If the sample requires shared storage space / storage type please state the type and volume (e.g. -20C, -80C?)

Please send Ivona (ivona.cetinic@nasa.gov) the method documentation as an attachment. Upload is not working!

Requester (name, institution)

Sample type (targeted parameters and methodology, use the space here to explain, or send Ivona an email - see below)

Additional data needed (if yes, write in what kind, if not write no)

If sample requires additional water collection, please estimate volume and frequency (time and depth)?

If the sample requires shared storage space / storage type please state the type and volume (e.g. -20C, -80C?)

Does sample requires extra hands for collection

Science question targeted

Please enter your email address.

Please enter your year (e.g. Junior, Senior, G1, G2, postdoc)

Please add any information that may impact your ability to participate in this course (caregiver, internet/device access, etc.)

Ken B.(this would be post doc activity for new measurement)

coordinate with 234Th group

see above; 5-10 L profiles 3? times per cruise surface to 500-1000m 8-12 depths

None. To be processed by new WHOI EXPORTS postdoc

NOTE- this is a new measurement from a postdoc who is already part of Buesseler's team. Norm has been told of this activity and use of tracer on board Sally Ride

Using polonium-210/lead-210 as tracer of C export

https://drive.google.com/open?id=1WFJWGjJP1MIVqZWRuoFlymiqtNWI_Sfb

Follow up with Ken B. Should the additional cast be done?

Complimentary to C and N analyses.

Should be able to use the same filter for C and N (split filter in half). If not, 2-5 L.

Prefer -80, but could be stored in the dark at room temperature depending on total P versus organic P

More water filtration conducted by Benitez-Nelson on Process ship.

Interested in measuring particulate P on all water column, pump, and sediment trap samples to examine how elemental stoichiometry changes with biological pump.

https://drive.google.com/open?id=1EtZiDWgCOPdJtEUyP47UdEqIax8h9Lsv

Follow up with Claudia and Ben Water column samples how much water is needed?

1. Sample Type: flow cytometry; glut-fixed seawater, 3x 2 ml (<20-40 µm pre-filter) per sample.
Sample Method: tube filled (flash frozen, if possible) and stored at -80C

2. Sample Type: viral omics
Sample Method: first filtration (0.2 µm; 142 mm PES), iron chloride flocculation, 2nd filtration (0.45 µm; 142 mm PES); filter storage at 4C
2x 20 L on filter 1 (0.2 µm filter with host cells), stored in 50 ml flacon @ -20C
2x 20 L on filter 2 (0.45 µm filter with virus floccs), stored in 50 ml flacon @ 4C

Link to Protocol: https://umich.app.box.com/s/pyhd8uhga6zhmm9qeaim19159wnsk5fo

Nothing in addition to what is currently planned.

We will sample in parallel with the microbial (euks, bac+arch) sampling teams. The hope is that we will take their filtrate, but recognize we will require greater volumes of water.

2x 50 ml falcon tubes per sample in 4C
2x 20 ml falcon tubes per sample in -20C
3x 2 ml cyrovials per sample at -80C

This will depend on who is sampling and bandwidth they have, but intention is for all sampling to be done by current crew.

The efficiency of the biological pump is determined by food web processes. We propose to include study of viruses to capture this major component of top down control on lower food web processes and their connection to biological pump efficiency. Using flow cytometric and 'omics (metagenomics, proteomics) approaches to capture viral abundances and diversity, we will address: Do measures of virus:host abundances, viral community composition and function, and topology of predicted virus-host ecological networks inform models of biological pump efficiency?

Broadly: Virus community composition, quantification*

* I found the “Funded_Measurement_Table_V6” file on the EXPORTS site. I see “including viral numbers” in FCM/Microscopy for heterotrophs and protists. If you are all set there, I won’t cover it—but happy to help if needed, we have a new FCM that’s great for virus counts, and a Bigelow-trained tech :-)

Also in this table I see mention of viral infections/dilutions, mitomycin C experiments. Those are pretty neat experiments we are doing in other systems, but we can’t tackle that with only the opportunistic water collections.

Heterotrophs.. check with susanne MD..

Three 12 point unfiltered water profiles (coordinate with 234Th group) of 60 ml; Subsamples from pumps 3x; possible subsamples from traps (1 -3 times; tbd depending upon other core needs).
Samples for comparison of Ba concentration and Ba isotopes in water column and suspended and sinking particles.

Hydrography and nutrients. In addition, relates most directly to 234Th and 210Po/210Pb, but as a reminerlization tracer it is linked to many other EXPORTS C flux parameters and proxies

Three 12 point profiles (coordinate with 234Th group) for water (60 ml)

No. Water stored room temp. Particles can be dried. Horner will provide containers.

Postdoc in Buesseler group will help coordinate all sampling and processing at sea (and work on analyses back at WHOI in collaboration with Horner lab)

How do upper ocean ecosystem characteristics determine the
vertical transfer of organic matter from the well-lit surface
ocean? This question will be tackled by measuring the abundance
and isotopic composition of barium—an emerging geochemical proxy
for organic carbon oxidation—in dissolved and particulate samples
collected in the upper water column.

Survey Ship (& trap samples from Process ship)

Parameter: N and O isotopic composition of dissolved nitrate (NO3-) analyzed via the 'denitrifier' method. Samples require seawater from Niskin bottle that has undergone the same filtration as samples for macro-nutrient (nitrate, phosphate, silicate, etc.) measurements. It is preferred that these samples come from the same casts and bottles as the macro-nutrient measurements. However, it is ok if filtration is not available. See attached methods document.

Basic hydrography, macronutrient data, shipboard ADCP

Samples are 30 mL. A full-depth profile is requested once during the cruise, with additional profiles from the upper 400 m as available.

0.5 cu ft of storage in -20C

Would make sense to take these on the Survey ship when macronutrients are collected, but if people are too busy I (Alyson) can try to coordinate it on the process ship.

How do upper ocean ecosystem characteristics determine the vertical transfer of organic matter from the well-lit surface ocean?

I don't care, just give me the samples

https://drive.google.com/open?id=19sKcilKq5-BtftT-3dMAJIVtfkR3Pj5D, https://drive.google.com/open?id=1YcnpV1i1NsLxNWcQPIaht6azXYsFXT1G

Susanne Menden-Deuer, URI

Samples to be filled with peristaltic pump and put in a solution to preserve RNA. No other details available at the moment.

1 L samples, three depths, three times per epoch (nine times during cruise).

Will need to be done by someone on hydro team or Roesler team

Transcriptome analysis to complement (and increase the footprint) of Menden-Deuer 'grazing marker gene' project on Process Ship.

Nicolas group water collection maybe?

Ken B. & Nuria Casacuberta (129-I analyses at ETH Switzerland)

8-10 surface water samples taken as we are underway back to Seattle. This is to measure 137Cs and 129I for comparisoin to similar samples taken in 2015

Looking at tracers of cross Pacific water transport from Fukushima in 2011

https://cmer.whoi.edu/recipe/cs-from-20l-using-knifc-pan-resin/