20151117_RAD_qPCR_data
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ABCDEFGHIJKLMNOPQRSTUVWXYZ
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Well / SetDyeContentDescriptionEfficiencyC(t)pMAvg C(t)Max C(t)Min C(t)C(t) SDAvg pMMax pMMin pMpM SD
Wellset Type
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NTCSBGnewSampleNTC21.99%29.471.67E-0629.4529.6829.220.191.72E-061.99E-061.46E-062.19E-07Replicate
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RAD 02SBGnewSampleRAD0297.28%12.610.171512.6813.2512.190.440.17070.22840.11060.04811Replicate
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RAD 03SBGnewSampleRAD0392.35%11.830.291411.8412.0711.690.170.29240.32030.24710.03234Replicate
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RAD04SBGnewSampleRAD0495.80%12.210.225312.2112.4112.090.140.22540.2450.19620.02106Replicate
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RAD06SBGnewSampleRAD0692.55%13.230.112413.2313.4213.10.130.11240.12230.098680.01Replicate
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RAD07SBGnewSampleRAD0797.92%9.211.7529.239.5890.261.7562.0261.3570.2882Replicate
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RAD08SBGnewSampleRAD08102.48%12.620.170112.6412.8212.360.20.16990.20360.14850.02413Replicate
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RAD14SBGnewSampleRAD1493.18%10.310.82610.3310.6110.080.220.8240.96610.67330.1197Replicate
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RAD17SBGnewSampleRAD1795.81%13.550.0901813.5613.8713.310.240.090640.10630.072130.01408Replicate
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RAD23SBGnewSampleRAD23103.14%12.520.18212.5712.97120.410.18380.26070.13420.05511Replicate
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RAD30SBGnewSampleRAD3095.31%14.70.0411114.7214.9814.290.310.041410.054360.033790.009201Replicate
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Std1SBGnewStandard101.14%5.67205.75.925.290.292020200Replicate
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Std2SBGnewStandard96.81%9.1329.149.498.90.252220Replicate
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Std3SBGnewStandard95.25%12.30.212.3112.512.130.150.20.20.20Replicate
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Std4SBGnewStandard92.98%15.70.0215.7115.8715.580.120.020.020.020Replicate
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Std5SBGnewStandard74.72%18.940.00218.9419.118.830.120.0020.0020.0020Replicate
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Std6SBGnewStandard68.43%22.660.000222.6822.9222.380.230.00020.00020.00020Replicate
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LIBRARY SUBMISSION CALCS
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Library
Avg. Conc. (pM)
KAPA Standards Fragment Size (bp)
Library Fragment Size (bp)
Size-adjusted Conc. (pM)
qPCR Dilution Factor
Library Stock Conc. (pM)
Library Stock Conc. (nM)
Halved (nM)
Conc (ng/uL)
20ng (uL)40ng (uL)AmountConc
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RAD 020.17154521660.466975903610000046,697.5946.7023.354.715816874.248.484.427244982
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RAD 030.29144521660.793450602410000079,345.0679.3539.678.0127640572.504.99
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RAD040.22534521660.613467469910000061,346.7561.3530.676.1951809953.236.46
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RAD060.11244521660.30605301210000030,605.3030.6115.303.0907161296.4712.94
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RAD071.7524521664.770506024100000477,050.60477.05238.5348.175575250.420.83
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RAD080.17014521660.463163855410000046,316.3946.3223.164.6773204054.288.55
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RAD140.8264521662.249108434100000224,910.84224.91112.4622.71291390.881.76
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RAD170.090184521660.245550361410000024,555.0424.5612.282.4797222478.0716.13
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RAD230.1824521660.495566265110000049,556.6349.5624.785.0045403514.007.99
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RAD300.041114521660.111938072310000011,193.8111.19NA1.13042117517.69
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85.83
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Well / SetDyeContentDescriptionEfficiencyC(t)pM
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A1SBGnewSampleRAD0291.19%13.250.1106
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A2SBGnewSampleRAD0297.13%12.590.1732
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A3SBGnewSampleRAD0298.84%12.190.2284
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A4SBGnewSampleRAD0394.87%11.740.3099
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A5SBGnewSampleRAD0384.76%11.690.3203
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A6SBGnewSampleRAD0391.29%12.070.2471
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A7SBGnewSampleRAD0496.92%12.410.1962
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A8SBGnewSampleRAD0495.54%12.090.245
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A9SBGnewSampleRAD0495.13%12.150.2351
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A10SBGnewSampleRAD0690.71%13.180.1161
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A11SBGnewSampleRAD0694.19%13.10.1223
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A12SBGnewSampleRAD0692.70%13.420.09868
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B1SBGnewSampleRAD07101.06%9.581.357
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B2SBGnewSampleRAD07112.08%92.026
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B3SBGnewSampleRAD0797.59%9.11.886
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B4SBGnewSampleRAD08103.76%12.360.2036
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B5SBGnewSampleRAD08100.24%12.730.1575
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B6SBGnewSampleRAD08103.12%12.820.1485
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B7SBGnewSampleRAD1489.67%10.610.6733
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B8SBGnewSampleRAD1495.79%10.080.9661
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B9SBGnewSampleRAD1493.06%10.30.8327
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B10SBGnewSampleRAD1792.16%13.310.1063
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B11SBGnewSampleRAD1794.79%13.490.09355
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B12SBGnewSampleRAD17102.71%13.870.07213
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C1SBGnewSampleRAD23109.52%12.970.1342
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C2SBGnewSampleRAD23107.87%12.740.1566
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C3SBGnewSampleRAD23112.07%120.2607
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C4SBGnewSampleRAD3092.01%14.290.05436
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C5SBGnewSampleRAD30113.49%14.980.03379
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C6SBGnewSampleRAD3085.14%14.890.03609
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C7SBGnewStandardStd1100.28%5.9220
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C8SBGnewStandardStd196.27%5.2920
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C9SBGnewStandardStd1109.63%5.8720
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C10SBGnewStandardStd2107.17%8.92
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C11SBGnewStandardStd296.14%9.052
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C12SBGnewStandardStd2101.15%9.492
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D1SBGnewStandardStd388.63%12.50.2
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D2SBGnewStandardStd395.46%12.290.2
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D3SBGnewStandardStd3100.33%12.130.2
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D4SBGnewStandardStd492.68%15.680.02
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D5SBGnewStandardStd499.20%15.580.02
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D6SBGnewStandardStd486.12%15.870.02
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D7SBGnewStandardStd579.85%19.10.002
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D8SBGnewStandardStd567.91%18.830.002
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D9SBGnewStandardStd576.29%18.880.002
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D10SBGnewStandardStd670.05%22.380.0002
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D11SBGnewStandardStd668.09%22.730.0002
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D12SBGnewStandardStd666.69%22.920.0002
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E1SBGnewSampleNTC19.95%29.451.71E-06
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E2SBGnewSampleNTC28.09%29.681.46E-06
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E3SBGnewSampleNTC18.58%29.221.99E-06
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20151117_RAD_qPCR_data
 
 
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