LAMP Primers UPDATED 12-17-2020

	A	B	C	D	E	F
1	FloodLAMP Contact Randy True randy@floodlamp.bio
2	from Rabe Cepko	Proc Natl Acad Sci 2020 Sep 29;117(39):24450-24458. doi: 10.1073/pnas.2011221117. Epub 2020 Sep 8.
3	Harvard University IP
4
5	All oligos 5' to 3'
6	6 Primer per set to be premixed at 30X concentration
7	All primers HPLC purified
8	Resuspended in DNAse RNAse free water, shipped on cold packs
9	Supplied in 1ml tubes
10	MOQ 25 x 1ml tubes (10K rxns at 2.5ul per reaction of 10X)
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12	As1e - ORF1ab (SARS-CoV-2)			30X μM	ml	total nmoles
13	As1e_FIP	TCAGCACACAAAGCCAAAAATTTATTTTTCTGTGCAAAGGAAATTAAGGAG	51	48	25	1200
14	As1e_BIP	TATTGGTGGAGCTAAACTTAAAGCCTTTTCTGTACAATCCCTTTGAGTG	49	48		1200
15	As1_F3	CGGTGGACAAATTGTCAC	18	6		150
16	As1_B3	CTTCTCTGGATTTAACACACTT	22	6		150
17	As1_LF	TTACAAGCTTAAAGAATGTCTGAACACT	28	12		300
18	As1_LB	TTGAATTTAGGTGAAACATTTGTCACG	27	12		300
19
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21	E1 - E Gene (SARS-CoV-2)
22	E1-FIP	ACCACGAAAGCAAGAAAAAGAAGTTCGTTTCGGAAGAGACAG	42	48
23	E1-BIP	TTGCTAGTTACACTAGCCATCCTTAGGTTTTACAAGACTCACGT	44	48
24	E1-F3	TGAGTACGAACTTATGTACTCAT	23	6
25	E1-B3	TTCAGATTTTTAACACGAGAGT	22	6
26	E1-LF	CGCTATTAACTATTAACG	18	12
27	E1-LB	GCGCTTCGATTGTGTGCGT	19	12
28
29	N2 - N Gene (SARS-CoV-2)
30	N2-FIP	TTCCGAAGAACGCTGAAGCGGAACTGATTACAAACATTGGCC	42	48
31	N2-BIP	CGCATTGGCATGGAAGTCACAATTTGATGGCACCTGTGTA	40	48
32	N2-F3	ACCAGGAACTAATCAGACAAG	21	6
33	N2-B3	GACTTGATCTTTGAAATTTGGATCT	25	6
34	N2-LF	GGGGGCAAATTGTGCAATTTG	21	12
35	N2-LB	CTTCGGGAACGTGGTTGACC	20	12
36
37	RNAseP (Human IC)
38	RNP-FIP	GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC	41	48
39	RNP-BIP	CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC	45	48
40	RNP-F3	TTGATGAGCTGGAGCCA	17	6
41	RNP-B3	CACCCTCAATGCAGAGTC	18	6
42	RNP-LF	ATGTGGATGGCTGAGTTGTT	20	12
43	RNP-LB	CATGCTGAGTACTGGACCTC	20	12
44
45	ActinB (Human IC)
46	ACTB-FIP	GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA	40	48
47	ACTB-BIP	CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC	38	48
48	ACTB-F3	AGTACCCCATCGAGCACG	18	6
49	ACTB-B3	AGCCTGGATAGCAACGTACA	20	6
50	ACTB-LF	TGTGGTGCCAGATTTTCTCCA	21	12
51	ACTB-B	CGAGAAGATGACCCAGATCATGT	23	12
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