Bio 79 schedule
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Spring 2019 tentative schedule for Bio 79
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Note: Subject to change! Please check back often.
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DateLectureLaboratory
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1/22IntroductionLaboratory Orientation/Documentation
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1/24pH and BuffersBuffers and Media Preparation
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1/29pH and Buffers cont.Buffers and Media Preparation cont.; Spectrophotometry, OD, and Aseptic Technique
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1/31Spectrophotometry and Optical Density (OD)No lab due to instructor illness
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2/5Spectrophotometry and Optical Density (OD) cont.Spectrophotometry, OD, and Aseptic Technique
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2/7Upstream Processing
Math homework 1 due
Effects of Culture Conditions on Bacterial Growth
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2/12Upstream Processing cont.Effects of Culture Conditions on Bacterial Growth, TF reading and data analysis
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2/14Upstream Processing cont.Optimization of induction and GFP production in E. coli
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2/19Upstream Processing cont.Optimization of induction and GFP production in E. coli
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2/21Upstream Processing cont.Optimization of induction and GFP production in E. coli, day 3; TF reading and data analysis in F170;
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2/26Upstream Processing cont.; Quiz 1 on math hw 1Start 1-L spinner flask; start 3-L insulin bioreactor
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2/28Upstream Processing cont. Monitor 1-L spinner; LB Agar Plate Media and Plate Pouring
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3/5Upstream processing cont.DHFR (Day 1): Streak plates, pgs. 121-123; clean 1-L spinner flasks
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3/7Upstream processing cont.; Lab notebook check 1DHFR (Day 2): Subculture and Induction, pgs. 121-123
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3/12Data AnalysisNo class due to instructor illness
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3/14Data Analysis cont.; Math homework 2 dueInstrumentation calibration: pipettes, scales, pH meters
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3/19Working in a Regulated Environment; Quiz 2 on Data AnalysiscGMP popcorn
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3/21Professional Development Day -- no classesSOLANO TOUR -- ARRIVE BY 11 AM
2001 N Village Pkwy, Vacaville, CA 95688
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3/26
Working in a Regulated Environment; Graph and table due
Continue DHFR (Day 3): Collecting Cell Pellet and Lysing Cells, pgs. 124-125; save samples for Gram stain
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3/28Midterm examDHFR (Day 4): Separation of Soluble and Insoluble Factions and SDS-PAGE Analysis of Induction, pgs. 126-129
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4/2Spring Break -- no classes
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4/4Spring Break -- no classes
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4/9Downstream ProcessingDHFR (Day 5); Affinity Purification of GST-DHFR-His Using AKTA, pgs. 139-151; Artel Pipetting Challenge Week
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4/11Downstream Processing cont.Continue Purification of DHFR; Start Sf9 cells media prep; Artel Pipetting Challenge Week (also with student PDA chapter at 6:30 pm)
DHFR (Day 6): Desalting and Prep for SDS-PAGE; Quantitation of Protein in Desalted Fractions, pgs. 152-157
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4/16Downstream Processing cont.Sf9 cell counts; poster project work in groups in F170 4-5 pm
DHFR (Day 7): SDS-PAGE of Purified Fractions, pgs. 158-160
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4/18Kelly Scientific talk in lecture; arrive promptly at 1 pm!Analysis of Sf9 culture; Poster project work in groups F170 3-5 pm
DHFR (Day 8): DHFR Enzymatic Assay, pgs. 201-209
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4/23Downstream Processing cont.Poster project work in groups in lab 2:30-5 pm
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4/25Downstream Processing cont.Poster project work in groups in lab 3:30-5 pm
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4/30Downstream Processing cont.More group work
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5/2Downstream Processing cont.General and aspetic gowning; resume on Canvas, LinkedIn profile w/ your pic and Laney BioMFG group join & connect to D. Bruce by
Sun., 5/5 at 12 midnight
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5/7Metrology and Calibration; Quiz 3; Poster final graphs due
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5/9ABPDU Tour 2-3 pm Address 5885 Hollis Street in Emeryville. BE THERE AT LEAST 15 MINS
EARLY TO REGISTER IN THE LOBBY.
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5/14Aseptic Processing & Environmental MonitoringFinalize posters in F170
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5/16QualityPoster session 4-5 pm
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5/21Final exam (starts at 12 pm); SOP Assignment (group, assigned topic) submitted via GoolgeDocs to douglasbruceatwork@gmail.com by Fri., 5/24 at 5 pm
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5/23
Optional: Solano Bachelor's graduation ceremony 4 pm in Vacaville
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