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A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples
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Ayaan Hossain1, Alexander C. Reis2, Sarthok Rahman5, and Howard M. Salis1-4 *
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1Bioinformatics and Genomics, 2Department of Chemical Engineering, 3Department of Biological Engineering, 4Department of Biomedical Engineering, 5Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
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*Corresponding author: Howard M. Salis, salis@psu.edu
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TABLE OF CONTENTS
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SUMMARY
Shared primer sequences, spike-in control sequences, CV and extraction-control amplicon sequences,
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N1 RT Primers
19200 Barcoded Reverse Transcriptase Primers for the N1 Amplicon in NC_045512.2
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N2 RT Primers
19200 Barcoded Reverse Transcriptase Primers for the N2 Amplicon in NC_045512.2
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RNaseP RT Primers
19200 Barcoded Reverse Transcriptase Primers for the RNAse P Amplicon in humans (RNA extraction control)
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96-set N1 RT Primers
Best 96 Barcoded Reverse Transcriptase Primers for the N1 Amplicon in NC_045512.2
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96-set N2 RT Primers
Best 96 Barcoded Reverse Transcriptase Primers for the N2 Amplicon in NC_045512.2
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96-set RNAseP RT Primers
Best 96 Barcoded Reverse Transcriptase Primers for the RNAse P Amplicon in humans (RNA extraction control)
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960-set N1 RT Primers
Best 960 Barcoded Reverse Transcriptase Primers for the N1 Amplicon in NC_045512.2
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960-set N2 RT Primers
Best 960 Barcoded Reverse Transcriptase Primers for the N2 Amplicon in NC_045512.2
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960-set RNAseP RT Primers
Best 960 Barcoded Reverse Transcriptase Primers for the RNAse P Amplicon in humans (RNA extraction control)
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Version 1.0
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March 20th 2020
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