CRL MIC Instrument List
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NicknameFull NameLocationDescriptionRecharge RateTraining Notes / FeesObjectivesUpright, Inverted, or LightsheetZ-stacksTime lapseLive Imaging CapabilitiesCleared Sample ImagingExcitation (lasers or filters if lamp-based)Multi-photon ExcitationSpinning Disk or Line-Scanning?Photo- stimulation (1p)Photo- stimulation (2p)405nm Excitation (aka DAPI)BrightfieldDIC or PhaseOther FeaturesNotesSoftware PlatformFree Viewer or Software
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LSA
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TrinityZeiss LSM 880 NLO AiryScan with Fast-AiryscanLSA 251Zeiss LSM-880 with Airyscan, Fast-Airyscan, and Coherent Chameleon Laser for 2p imaging$45/hr$240 (2 hours)5x air, 20x air, 20x/1.0 W, 20x SCALE, 40x W, 63x/1.4 oilUprightXXXYes, 20X SCALE objective405nm, 458nm, 488nm, 514nm, 561nm, 591nm, 635nm690-1040nm-Point-scanningPoint-scanningXyes, T-PMTGaASP detectors in confocal and NDD, AiryScan for high resoluition and increased sensitiityZen 2Zen Lite
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RachelZeiss LSM 780 NLO AxioExaminerLSA 251The Zeiss LSM 780 has a 32-channel spectral GaAsP detector, a Spectra-Physics MaiTai laser for 2-photon imaging, and a large fixed stage for combined electrophysiology and imaging.$45/hr$220 (2 hours)5x, 20x/1.0 dipping, 40x/1.4 oil, 63x/1.4 oil UprightXXIn vivo++; electrophysiology5x air only405nm, 458nm, 488nm, 514nm, 561nm, 635nm700-1040nm-Point-scanningPoint-scanningXyes, T-PMTDODTSingle scan spectral detection (linear unmixing); GaAsP detector (very sensitive); FRET; FRAP; FCSLarge fixed stage for combined electrophysiology & imaging; high resolution, high sensitivityZen 2010Zen Lite
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DeckardZeiss LSM 510 NLO AxioVert with FLIMLSA 251The MIC 510 AxioVert, Deckard, is a 3-channel confocal, 1-channel multi-photon, and 1-channel fluorescent lifetime system, complete with 1-photon and 2-photon excitation.$40/hr $300 + EH&S Laser Training + Laser Alignment + FLIM if needed10x air, 25x multi-immersion, 40x oil, 63x oil, 100x oilInvertedXXLive cell incubation with CO2, humidity, and temperature control-458nm, 477nm, 488nm, 514nm, 543nm, 633nm700-1020nm-Point-scanningPoint-scanningyes, with doubler onlyyes, T-PMTDIC and PhaseFLIM; FRET; FRAP; FCS; motorized stageMore intensive training needed - usually 3 sessions +Zen 2009Zen Lite
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Enterprise3i Upright SDC with SLMLSA 251This unique spinning disk confocal is on an upright stand great for zebrafish, drosophila and brain slice work and incorporates both 1-photon and 2-photon optical stimulation devices.$45/hr$200 (2 hours) + EH&S Laser Training5x, 20x water, 40x (immersion?)UprightXXSlice or small organisim (zebrafish, drosophila, explant samples, etc.)-488nm, 561nm-spinning disk405, 477, 594nm SLM780-920nm SLMPhasor onlyyes, through SDCUnique upright SDC with Phasors for optogenetics or photo- uncaging experiments-Slidebook 6Slidebook Reader
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BoomerZeiss LSM 510 META AxioPlanLSA 251The 510 Axioplan is a great system for fixed sample laser scanning confocal in green, red, and far-red.$20/hr$125 (1.5 hours)10x air, 20x dipping, 40x dipping, 40x oil, 63x oilUprightXX--458nm, 488nm, 514nm, 543nm, 633nm--Point-scanning--yes, T-PMTSpectral detection (linear unmixing) ++; FRET; FRAP-AIM 3.5
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BayMaxZeiss Axio Scan.Z1LSA 251Automated slide scanner for fluorescence and brightfield stains. Information about slide scanning services.$10/hr (unassisted), $30/hr we scan for you$150 (1.5-2 hours)5x, 10x, 20x-yes-No, fixed, sealed slides only-No lasers. Filter cubes for DAPI (FS 49), eGFP (FS 38 HE), Cy 3 (FS 43 HE), mPlum (FS 64 HE), Cy5 (FS 50)----yes, color camera1"x3" or 2"x3" sealed slides onlyZen Blue 2012Zen Lite
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Johnny-5Leica M205 Fluorescent StereoscopeLSA 251The Leica M205 is a fluorescent stereoscope used for screening live organisms. Currently, there is no camera attached.N/C5 minute introduction $01.0x, 1.6xUpright----No lasers, filter cubes for CFP, GFP, YFP, and DS-Red----Xyes, but no cameraApochromatic 20.5:1 zoomcan use well plates; no camera-
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DeepThoughtGPU-processing workstation with Imaris, Matlab, FIJILSA 251High-end Windows10 workstation for image visuatlization and analysis: Bitplane Imaris, Matlab, ImageJ, FIJI, Zeiss Zen, and Adobe Suite$7/hrno charge N/A
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HAL 9000Mac Pro WorkstationLSA 251Mac Pro workstation software for image rendering and analysis: Matlab, ImageJ, FIJI, Zeiss Zen, and Adobe Suite$7/hrno charge for 30 minute introN/A
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NeoZeiss LSM 880 NLO OPOLSA 211Zeiss LSM-880 with Coherent OPO Laser for near-IR deep tissue imaging$45/hr$240 (2+ hours)5x, 20x/1.0 W dipping, 63x/1.4 oilUprightXXX405nm, 458nm, 488nm, 514nm, 561nm, 591nm, 635nm690 - 1300nm-Point-scanningPoint-scanningXYes, T-PMTsuper sensitive GaAsP detectors in scanhead and NDD, fixed stageZen 2Zen Lite
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Millennium Falcon3i/Sutter In Vivo SLMLSA 211This system is uniquely designed for optogenetic research for in vivo studies. Special training is required.$45/hr$220 (2+ hrs) + EH&S Laser Training + Animal Use Registration20x W dipping, 40x W dippingUprightXXIn vivo+++-2p only, no visible lasers690-1040nm-700-1040nm SLM-no-uniquely designed for optogenetic, in vivo studiesSlidebook 6Slidebook Reader
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SerenityZeiss LightSheet Z1LSA 211The Zeiss LightSheet Z1 is a selective plane illumination microscope (SPIM), excellent for imaging zebrafish, drosophila, and other small transparent organisms$40/hr ($7/hr overnight and weekend)$220 (2-3 hours)5x, 20x W dipping, 40x WLightsheetXXXIn vivo++ (small organisms); Live cell incubation, heating & cooling-405, 488, 561, and 635nm-Selective Plane Illumination--Xyes, IR LEDsHeating and cooling (what are the temp ranges??)multiview; planar illuminationZen 2012 LSZen Lite
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ZoomZeiss AxioZoom Fluorescent StereoscopeLSA 211The AxioZoom is large-field fluorescent imaging station for screening or large sample size collection.$7/hr$75 (1 hour)1.0x, 2.3x (both air)UprightXXSure.Yes, low mag, WFNo lasers; filters for DAPI, CFP, GFP, YFP, and RFP----Xyes, transmitted or reflectedZoom factor of 7X-112XXY stitching capable; can use well platesZen Blue 2012 Zen Lite
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EveZeiss 5-LIVE AxioSkopLSA 246The MIC 5-LIVE is a specialized upright, fixed-stage line-scanning confocal system for doing very, very fast imaging.$40/hr$200 (2 hrs)10x air, 20x water, 40x water, 63x waterUprightXXVery fast acquisition-440nm, 488nm, 532nm, 635nm-lineline only-440nmyesFRET; Spectral detection (linear unmixing) +; very fast image acquisition with Piezo-Z scanning-Zen 2009Zen Lite
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MaxAndor/Nikon Spinning DiskLSA 338The Nikon/Andor system has the capabilities of spinning disk confocal microscope, TIRF, and photo-activation, all with a full-enclosure CO2 and temperature incubator.$20/hr ($7/hr overnight and weekend)$200 (2 hrs)40x air, 60x oil, 60x oil (TIRF), 100x oil, 100x oil (TIRF)InvertedXXLive cell incubation with CO2, humidity, and temperature control-405nm, 488nm, 561nm-spinning disk405nm DMD-XyesTIRF; FRAPXY stitching capable; can use well platesMetamorph
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The HerzmarkNikon Wide FieldLSA 432The Herzmark (previously known as the Live Cell Scope) is a wide-field epifluorescence scope equipped with DIC optics and a live-cell chamber. $25/hr$220 (2 hours)4x air, 10x air, 20x air, 40x oil, 60x oilInvertedN/AXLive cell incubation with CO2, humidity, and temperature control-N/A----Xyes, DICNew Computer!Nikon ElementsNikon Elements Viewer
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DeepPeekZeiss 7-MPLSA 445Zeiss 7-MP multi-photon only system with 4 channel PMT and Coherent Chameleon laser$50/hr$220 (2 hours)20x/1.0 W dippinguprightXXin vivo, brain slice, 2-photon, incubation heating & CO2 bubbling perfusion pump-No visibile lasersCoherent Chameleon 2p laser only (700-1000nm)-Zen 2010Zen Lite
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LKS
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MorpheusZeiss LightSheet for CLARITYLKS 230-CNew February 2015$40/hr ($7/hr overnight and weekends)$220 (3 hours)20X CLARITY objective, 20X Water, 5X CLARITYLightsheetXXYes, but recommend using Serenity which is water-based onlyYes, 20X or 5X CLARITY objective405nm, 440nm, 488nm, 514nm, 561nm, 642nm-Lighsheet--XIR-LEDoffline workstation with Arivis software
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Morpheus ProcessingZeiss workstation for data processingLKS 230 Offline workstation with Zen and arivis for lightsheet image processing, rendering, and analysisN/CN/CN/A
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Optimus PrimeZeiss LSM 710 AxioObserverLKS 361The LSM 710 is a 34-channel spectral laser scanning confocal microscope with CO2 and temperature incubation chamber for live cell work.$40/hr ($8/hr overnight and weekends)Standard $25010x air, 20x air, 40x air, 40x water, 40x oil, 63x oilInvertedXXLive cell incubation & CO2-405nm, 458nm, 488nm, 514nm, 561nm, 591nm, 635nm--Point-scanning-Xyes, T-PMTPolarization / Anisotropy; Spectral detection (linear unmixing) +XY stitching capable; can use well platesZen 2010Zen Lite
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MegatronNikon PALM with AOLKS 361This French-designed super-resolution system incorporates unique laser control for TIRF-based localization microscopy and adaptive optics for z-position information. It also has a spinning disk confocal, a full incubation enclosure, and a micro-injection system.$45/hr$220 (2 hours)InvertedXXLive cell incubation & CO2-405nm, 488nm, 561nm, 637nm-spinning disk 405, 488nm single spot only-XyesTIRF, Super ResolutionXY stitching capable; can use well plates; microinjection capabilityNikon ElementsNikon Elements Viewer
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IncuCyteEssen IncuCyte ZoomLKS 361The IncuCyte is a fully automated imager which sits inside the incubator, providing exceptionally stable long-term imaging. It can acquire images in Phase Contrast, as well as Green and Red fluorescence, at 4X, 10X, or 20X.$50/day$120 (~1 hour)4x, 10x, 20x (all air)Inverted-XLive cell incubation & CO2 (in incubator)-No lasers, filters for Green & Red fluorescence-----yes, Phasephase contrast, green & red epifluorescence; multi-day imaging; remote access/controlwhole well imaging possibleIncuCyte Zoomftp://50.201.185.115/Zoom/2016A/Base/IncuCyteZOOM2016AGuiUsesWebSetup.zip
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VincentWindows Offline WorkstationLKS 361Offline workstation to access the IncuCyte Zoom, or to use offline Zen Blue Software. N/C
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PrisZeiss LSM 510 META NLO AxioImagerLKS 261 (not 361)The MIC 510 AxioImager is an upright confocal and multiphoton laser scanning microscope with a Spectra-Physics DeepSee laser.$40/hr ($7/hr overnight and weekends)$200 (2 hours)10x air, 20x dipping, 40x dipping, 63x dipping, 40x oil, 63x oilUprightXXIn vivo+; physiology10x air only458nm, 488nm, 514nm, 543nm, 633nm700-1040nm-Point-scanningPoint-scanning2p onlyyes, T-PMTSpectral detection (linear unmixing) ++; FRET; FRAPExcitation profiling; up to 8 color simultaneouslyZen 2009Zen Lite
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Barker Hall
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Wall-ENikon Spinning Disk ConfocalBarker 636Nikon TI with PFS and spinning disk confocal, incubation and motorized stage$20/hr ($7/hr overnight and weekend)$200 (2 hrs)InvertedXXX-405nm, 488nm, 561nm, 635nm-spinning disk--Xyes (?)??Nikon Elements
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