| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | AA | AB | AC | |
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1 | Manuscript Status | First Author | Publication Date | Location | Sample Size and Location | Sample Type | Sample Volume Processed | Concentration Method | Recovery | Assay Type | Confirmation | Reference | |||||||||||||||||
2 | Correspondence (Lancet Gastroenteral Hepatol) | Lodder, Willemijn | April 1, 2020 | Airport Schiphol, Netherlands | raw sewage; sample size not reported | 24 h composite | Not reported | Not reported | Not reported | Not reported | Not reported | https://www.thelancet.com/journals/langas/article/PIIS2468-1253(20)30087-X/fulltext | |||||||||||||||||
3 | Peer-reviewed (mSystems) | Wu FQ | April 7, 2020 | Massachusetts, USA | 14; 2 catchments x 7 days, raw influent | 24 hr composite | 40 mL of filtrate | PEG precipitation with pre-filtration (0.2 um) + pasteurization | Not reported | RT followed by qPCR: S gene; N1, N2, N3 | PCR product sequencing (S gene) | https://msystems.asm.org/content/5/4/e00614-20 | |||||||||||||||||
4 | Peer-reviewed (International Journal of Infectious Diseases) | Wang, Jie | 9 April 2020 | Zhejiang University, China | 5; isolation ward sewage; Inlets of hospital sewage disinfection pool (3), preprocessing disinfection pool (1); outlet (1) | grab samples | Not reported | Not reported | Not reported | RT-qPCR: virus culture | None posivitve by culture | https://doi.org/10.1016/j.ijid.2020.04.024 | |||||||||||||||||
5 | Peer-reviewed (STOTEN) | Ahemd, Warish | April 15, 2020 | Brisbane, Australia | 9; pump station (1); WWTP A (6) & B (2) | grab samples; composite samples | 100 - 200 mL | Electronegative membrane; ultrafiltration (Centricon) | Not reported | RT-qPCR: N_Sarbco, NIID_2019-nCoV | RT-qPCR product sequencing (Sanger, Illumina) | https://doi.org/10.1016/j.scitotenv.2020.138764 | |||||||||||||||||
6 | Preprint (medRxiv) | Nemudryi, Artem | April 20, 2020 | Bozeman, Montana, USA | 7; pre-treated wastewater (influent) | grab and 24 h composite (flow weighted) | 500 mL | Sequential filtration: 20 um, 5 um, 0.45 um; ultrafiltration | Not reported | RT-qPCR: N1, N2 | RT-PCR and electrophoresis; Sanger sequencing | https://www.medrxiv.org/content/10.1101/2020.04.15.20066746v1 | |||||||||||||||||
7 | Preprint (medRxiv) | Randazzo, Walter | April 29, 2020 | Region of Valencia, Spain | 24; 15 influent, 9 treated effluent | Not reported | 200 mL | Flocculation precipitation | MgV (>1%) | RT-qPCR; N1, N2 | Not reported | https://www.medrxiv.org/content/10.1101/2020.04.23.20076679v2 | |||||||||||||||||
8 | Preprint (medRxiv) | Zhang, Dayi | April 30, 2020 | Wuhan, China | 6; 3 influent, 3 effluent | grab samples | Not reported | PEG precipitation | Not reported | RT-qPCR; CCDC-ORF1, CCDC-N | Not reported | https://www.medrxiv.org/content/10.1101/2020.04.28.20083832v2 | |||||||||||||||||
9 | Preprint (medRxiv) | Bar-Or, Itay | May 1, 2020 | Israel | 26; 17 WWTP influent; 4 from sewer network; 2 hosptial effluent; 3 isolation facility effluent | 24 hr composite; grab samples | 1 mL; 250 mL to 1 liter | direct extraction; PEG or alum precipitation; ultrafiltration | Not reported | RT followed by qPCR: E gene | Not reported | https://www.medrxiv.org/content/10.1101/2020.04.26.20073569v1 | |||||||||||||||||
10 | Peer-reviewed (STOTEN) | Rimoldi, Sara Giordana | May 5, 2020 | Milano, Italy | 8; 4 raw influent, 2 treated, 2 river samples | grab samples | 200 uL | glass fiber filtration + membrane filtration | Not reported | qualitative PCR assay; N gene; ORF1ab gene; E gene | WGS of isolated strains; cell culture with VERO E6 | https://www.sciencedirect.com/science/article/pii/S0048969720344405 | |||||||||||||||||
11 | Preprint (medRxiv) | Wurtzer, S | May 6, 2020 | Paris, France | 31; 23 raw influent, 8 treated effluent time series | Not reported | 11 mL | ultracentrifugation | Not reported | RT-qPCR; E_Sarbeco | RT-qPCR of RdRp region | https://www.medrxiv.org/content/10.1101/2020.04.12.20062679v2 | |||||||||||||||||
12 | Preprint (medRxiv) | Kocamemi, Bilge Alpaslan | May 6, 2020 | Istanbul, Turkey | 9; 7 WWTP influent; 2 manholes | 24 hr composite; grab samples | 250 mL | Ultrafiltration (high inhibition); PEG precipitation | 1 to 1.5 log loss; avian coronavirus | RT-qPCR; N1, N2, N3 | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.03.20089417v1 | |||||||||||||||||
13 | Peer-reviewed (Water Research) | Randazzo, Walter | May 12, 2020 | Region of Murica, Spain | 72; 6 different WWTP; influent (42), secondary effluent (18), tertiary effluent (12) | grab samples | 200 mL | Adsorption-precipiration (aluminum hydroxied) | Influent: PEDV (10%), MgV (10%) | RT-qPCR: N1, N2, N3 | Not reported | https://www.sciencedirect.com/science/article/pii/S0043135420304796 | |||||||||||||||||
14 | Preprint (medRxiv) | Kocamemi, Bilge Alpaslan | 16 May 2020 | Istanbul, Turkey | primary sludge (2); waste activated sludge (7) | grab samples | 250 mL | PEG adsorption | Not reported | RT-qPCR: RdRp | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.12.20099358v1 | |||||||||||||||||
15 | Preprint (medRxiv) | Zhang, Dayi | 19 May 2020 | Wuhan, China | 28; wastewater samples from different treatment tanks; adjusting tank; raw medical wastewater; MBBR; sedimentation tank; | grab samples | 400 mL | PEG precipitation | Not reported | RT-qPCR; CCDC-ORF1, CCDC-N | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.12.20097105v2 | |||||||||||||||||
16 | Peer-reviewed (ES&T Letters) | Medema, Gertjan | May 20, 2020 | Netherlands | 30; WWTP influent in six cities (26) and airport (4) | 24 h composite (flow weighted) | 0.35 to 2.73 mL | ultrafiltration (Centricon) | F-specific RNA phage (73%); Dengue virus internal control extraction and RT-qPCR was 30.4% | RT-qPCR: N1, N2, N3, and E gene | High resolution electrophoresis with a bioanalyzer | https://pubs.acs.org/doi/10.1021/acs.estlett.0c00357 | |||||||||||||||||
17 | Peer-reviewed (STOTEN) | La Rosa, Giuseppina | May 21, 2020 | Milan and Rome, Italy | 12; WWTP influent (12) | 24 h composite | 250 mL | 30 min at 56C; PEG-dextran; | Not reported | nested RT-PCR: ORF1ab + novel ORF1ab; S gene; RT-pPCR: RdRP gene | Electrophoresis of PCR products and sequencing | https://www.sciencedirect.com/science/article/pii/S0048969720331727 | |||||||||||||||||
18 | Preprint (medRxiv) | New Haven, CT | 22 May 2020 | New Haven, CT, USA | Primary sludge | grab samples | 2.5 mL | direct extraction | Not reported | RT-qPCR: N1, N2 | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.19.20105999v2 | |||||||||||||||||
19 | Preprint (medRxiv) | Green, Hyatt | May 23, 2020 | Syracuse, NY | 22; 11 points (WWTP, pump stations, interceptor lines) x 2 time points | 24 h composite | 20 mL | ultracentrifugation (150,000 x g); | (12%) samples spiked with SARS-CoV-2 (580 gc/mL); crAssphage DNA recovery | RT-qPCR: IP2, IP4 | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.21.20109181v1.full | |||||||||||||||||
20 | Preprint (medRxiv) | Balboa, Sabela | 26 May 2020 | Ourense, Spain | influent (15), primary sludge, secondary sludge, sludge thickener, digested sludge (35) | grab samples | 100 mL water; 50 mL sludge | ultrafiltration; PEG precipitation | MS2 | RT-qPCR: RdRp, N, E | Not reported | https://www.medrxiv.org/content/10.1101/2020.05.25.20112706v1 | |||||||||||||||||
21 | Preprint (Preprints.org) | Fernández-de-Mera, Isabel | 27 May 2020 | Isolated Rural Village, Ciudad Real, Castilla - La Mancha, Spain | 1; collected from main sewage drain | grab sample | 5 mL processed | centrifugation | Not reported | RT-qPCR: E gene, IP2, IP4, | Not reported | https://www.preprints.org/manuscript/202005.0450/v1 | |||||||||||||||||
22 | Preprint (medRxiv) | Döhla, Manuel | 2 June 2020 | 21 quarantined households; Germany | 66; "wastewater" samples - siphon water (u-bend) from washbasin, toilet, showers | grab samples | Not reported | Not reported; appears to be direct extraction | Not reported | RT-qPCR; E_Sarbeco, RdRp | Attempted culture (no positives) | https://www.medrxiv.org/content/10.1101/2020.05.28.20114041v1 | |||||||||||||||||
23 | Preprint (medRxiv) | Sharif, Salmaan | 5 June 2020 | Pakistan | 78; wastewater samples from 38 districts across Pakistan | grab samples | 500 mL | Centrifugation + PEG-dextran + centrifugation | Not reported | RT-qPCR; ORF1ab, ORF-1, N, E gene | Sequencing of partial ORF1a in select samples | https://www.medrxiv.org/content/10.1101/2020.06.03.20121426v2 | |||||||||||||||||
24 | Peer-reviewed (STOTEN) | Haramoto, Eiji | 7 June 2020 | Yamanashi Prefecture, Japan | 10; Influent (5) and secondary effluent (5), (river, 3) | grab samples | 200 mL to 5,000 mL | electronegative membrane vortex; membrane adsorption-direct RNA extraction | MS2 for extraction; NIC for inhibition; PMMoV was measured to assess relative recovery between concentration methods | RT-qPCR; nested PCR; two-step; N_Sarbeco, NIID_2019-nCoV_N, N1, N2, ORF1a, S protein | Gel eletrophoresis | https://www.sciencedirect.com/science/article/pii/S0048969720339279 | |||||||||||||||||
25 | Preprint (medRxiv) | Hata, Akihiko | 12 June 2020 | Ishikawa and Toyama, Japan | 27 influent samples | grab sample (AM peak flow) | 100 mL; 80 mL & 20 mL subsample | PEG precipitation (80 mL); | Murine norovirus (molecular process control); indigeneous F-phage | RT-qPCR; nested RT-PCR: N1, N2, and N3 | Gel electrophoresis; sequencing of amplicons | https://www.medrxiv.org/content/10.1101/2020.06.09.20126417v2 | |||||||||||||||||
26 | Preprint (medRxiv) | Chavarria-Miró, Gemma | 13 June 2020 | Barcelona, Spain | influent from 2 WWTP (7 each); frozen archival samples (5); sewer grab samples (8) | composite; grab samples | 800 mL | PEG precipitation | Not reported | RT-qPCR: RdRp (IP2, IP4), E, N1 and N2 | Not reported | https://www.medrxiv.org/content/10.1101/2020.06.13.20129627v1 | |||||||||||||||||
27 | Peer-reviewed (STOTEN) | Kumar, Manish | 18 June 2020 | Ahmedabad, India | 4; Influent (2), final effluent (2) | composite sample from 3 grab samples | 50 mL; two 25 mL aliquots | Centrifugation + prefiltration + PEG (OR) 96-well filter plate | MS2 phage inhibition control; | RT-qPCR; ORF1ab, N, and S (presence/absence kit) | Not reported | https://www.sciencedirect.com/science/article/pii/S0048969720348555 | |||||||||||||||||
28 | Preprint (medRxiv) | Arora, Sudipti | 20 June 2020 | Jaipur, India | 25; municipal WWTP influent (12) and effluent (6), hospital wastewater influent (5) and effluent (2) | not reported | 1 mL & 50 mL | (1) direct extraction (2) 60C PEG "adsorption" | Not repoted | RT-qPCR; S, E, ORF1ab, RdRp, N | Not reported | https://www.medrxiv.org/content/10.1101/2020.06.18.20135277v1 | |||||||||||||||||
29 | Preprint (medRxiv) | Wu, Fuqing | 23 June 2020 | Massachusetts, USA | 116 primary influent samples | https://www.medrxiv.org/content/10.1101/2020.06.15.20117747v2 | |||||||||||||||||||||||
30 | Preprint (medRxiv) | La Rosa, Giuseppina | 26 June 2020 | Milan/Lombardy, Turin/Piedmont, Bologna/Emilia Romagna, Italy | 40+24; Influent | composite | 250 mL | 56C 30 minutes; PEG-dextran | Separate HCoV spiking experiments; | RT-qPCR; nested RT-PCR; ORF1ab, E, RdRp | Gel eletrophoresis; sequencing | https://www.medrxiv.org/content/10.1101/2020.06.25.20140061v1 | |||||||||||||||||
31 | Peer-reviewed (STOTEN) | Sherchan, Samendra | 28 June 2020 | Louisiana, USA | 15; wastewater samples; primary influent (7), secondary effluent (4), final effluent (4) | composite (9), grab (6) | 250 mL; 100mL; 750 mL (secondary eff) | (1) ultrafiltration (2) adsorption-elution | Phi 6 (extraction-RT-PCR control) | RT-qPCR: N1 and N2 | Not reported | https://www.sciencedirect.com/science/article/pii/S0048969720341437 | |||||||||||||||||
32 | Preprint (medRxiv) | Fongaro, Gislaine | 29 June 2020 | Santa Catalina, Brazil | 6; raw sewage from collection system | grab samples | 25 mL | reference, but no details | murine norovirus | RT-qPCR: N1, S, and RdRp | Not reported | https://www.medrxiv.org/content/10.1101/2020.06.26.20140731v1 | |||||||||||||||||
33 | Preprint (medRxiv) | Ampuero, Manuel | 3 July 2020 | Santiago, Chile | 16; 2 WWTP: influent (8) and effluent (8) | composite | 42 mL | ultrafiltration | human JC polyomavirus (qualitative) | RT-qPCR: ORF1ab, S, and N | Not reported | https://www.medrxiv.org/content/10.1101/2020.07.02.20145177v1 | |||||||||||||||||
34 | Preprint (medRxiv) | Vallejo, Juan | 6 July 2020 | A Coruna, Spain | Not reported | composite & grab (2h intervals) | 100 mL | 0.22 um prefilter followed by Amicon 30 kDa | not reported | RT-qPCR: N gene | Not reported | https://www.medrxiv.org/content/10.1101/2020.07.02.20144865v2.article-info | |||||||||||||||||
35 | Peer-reviewed (STOTEN) | Guerrero-Latorre, Laura | 7 July 2020 | Quito, Ecuador | 3 samples | grab samples | 2 liters | skimmed milk flocculation | human adenovirus | RT-qPCR: N1 and N2 | Not reported | https://www.sciencedirect.com/science/article/pii/S0048969720343564 | |||||||||||||||||
36 | Peer-reviewed (Mem Inst Oswaldo Cruz) | Prado, Tatiana | 9 July 2020 | Niteroi, Rio de Janeiro Brazil | 12 raw sewage samples; WWTP; hospital effluent; collection system | composite samples (10hr) | not reported | pasteurization; ultracentrifugation | Not reported | RT-qPCR: N2 | Not reported | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100412&lng=en&nrm=iso&tlng=en | |||||||||||||||||
37 | Preprint (medRxiv) | Trottier, Jule | 9 July 2020 | Montpellier, France | 6; raw wastewater composite | composite | 50 mL | Vivaspink 50 kDa MWCO filter membrane | No process control; Ebola RNA standard for extraction & RT-PCR | RT-qPCR: N1, N3 | Not reported | https://www.medrxiv.org/content/10.1101/2020.07.08.20148882v1 | |||||||||||||||||
38 | Preprint (medRxiv) | Curtis, Kyle | 11 July 2020 | Norfolk, VA, USA | 39; 36 two hour grab samples; 3 composite samples | composite (3) and grab samples (36) | 50 mL raw; 200 mL final effluent | electronegative filtration | Hep G (extraction); process not reported | RT-ddPCR: N1, N2, N3 | Not reported | https://www.medrxiv.org/content/10.1101/2020.07.10.20150607v1 | |||||||||||||||||
39 | Preprint (medRxiv) | Jorgensen, Alissa | 11 July 2020 | Denmark, France, Belgium | 78 samples from 18 sites; 11 Danish cities, 2 Danish hospitals; 1 French WWTP; 4 Belgian hospitals | composite | not reported | Various precipitation protocols | murine norovirus | Eurofin Technologies VIRSeek RT-PCR | Not reported | https://www.medrxiv.org/content/10.1101/2020.07.10.20150573v2 | |||||||||||||||||
40 | Preprint (Research Square) | Weidhaas, Jennifer | 15 July 2020 | Utah, USA | 126 samples from 10 WWTP; | 24hr flow weighted composite | not reported | pasteurization + 4500 g centrifugation; supernatant ph 3.5 electronegative membrane filtration | RT-qPCR: N1 and N2 | Not reported | https://www.researchsquare.com/article/rs-40452/v1 | ||||||||||||||||||
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