| A | B | C | D | E | F | G | H | I | J | S | T | U | V | W | X | Y | Z | AA | AB | AC | AD | AE | ||
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1 | https://twitter.com/kcorazo/status/1275703757112819713 | ⬅️ about this project | ||||||||||||||||||||||
2 | Item | Manufacturer | ID | Source/note | Price other | Price USD | Source/note | # req | Price | Category total | ||||||||||||||
3 | Reagents and flow cells | $ 5,184.25 | ||||||||||||||||||||||
4 | R9.4.1 flow cells | ONT | FLO-MIN106 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 1000 | 2 in the starter kit | 1 | $ 1,000.00 | ||||||||||||||||
5 | Extraction kit (2 options) | |||||||||||||||||||||||
6 | Zymo Quick-RNA Viral Kit | Qiagen | R1034 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 1 | $ - | no need | |||||||||||||||||
7 | QIAamp Viral RNA Mini | Thermo | 52904 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 1,170.00 | Small batch (52904) unavailable | 1 | $ 1,170.00 | no need | |||||||||||||||
8 | SuperScript IV (50 rxn) | Thermo | 18090050 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 230 | 287.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 287.50 | purchase | ||||||||||||||
9 | dNTP mix (10 mM each) | Thermo | R0192 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 82 | 102.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 102.50 | purchase | ||||||||||||||
10 | Random Hexamers (50 µM) | Thermo | N8080127 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 91 | https://www.fishersci.com/shop/products/random-hexamer-primers/n8080127 | 1 | $ 91.00 | ||||||||||||||||
11 | RNase OUT (125 rxn) | Thermo | 10777019 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 163 | 203.75 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 203.75 | |||||||||||||||
12 | Q5 Hot Start HF Polymerase | NEB | M0493S | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $137.00 | https://www.neb.com/products/m0493-q5-hot-start-high-fidelity-dna-polymerase#Product%20Information | 1 | $ 137.00 | ||||||||||||||||
13 | NEBNext Ultra II End-prep NEB E7546S | NEB | E7546S | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 729 | 911.25 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 911.25 | |||||||||||||||
14 | NEBNext Quick Ligation Module | NEB | E6056S | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | 317 | 396.25 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 396.25 | |||||||||||||||
15 | Native Barcoding Expansion Kit 1-12 | ONT | EXP-NBD104 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $288.00 | https://store.nanoporetech.com/catalog/product/view/id/298/s/native-barcoding-expansion-1-12/category/28/ | 1 | $ 288.00 | ||||||||||||||||
16 | Native Barcoding Expansion Kit 13-24 | ONT | EXP-NBD114 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $288.00 | https://store.nanoporetech.com/native-barcoding-expansion-13-24.html | 1 | $ 288.00 | ||||||||||||||||
17 | Sequencing Auxiliary Vials | ONT | EXP-AUX001 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $99.00 | https://store.nanoporetech.com/catalog/product/view/id/395/s/sequencing-auxiliary-vials/category/28/ | 1 | $ 99.00 | ||||||||||||||||
18 | Short Fragment Buffer Expansion kit | ONT | EXP-SFB001 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $30.00 | https://store.nanoporetech.com/sfb-expansion.html | 1 | $ 30.00 | ||||||||||||||||
19 | Flow Cell Priming Kit | ONT | EXP-FLP002 | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn/materials | $180.00 | https://store.nanoporetech.com/flow-cell-priming-kit-xl.html | 1 | $ 180.00 | ||||||||||||||||
20 | ||||||||||||||||||||||||
21 | Equipment and Containment | $ 11,225.00 | ||||||||||||||||||||||
22 | 1000µL pipettes | Gilson | 10387322 | https://artic.network/ebov/ebov-seq-kit.html | 210 | 262.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 525.00 | |||||||||||||||
23 | 100µL pipettes | Gilson | 10442412 | https://artic.network/ebov/ebov-seq-kit.html | 210 | 262.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 525.00 | |||||||||||||||
24 | 10µL pipettes | Gilson | 10236072 | https://artic.network/ebov/ebov-seq-kit.html | 210 | 262.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 525.00 | |||||||||||||||
25 | 2µL pipettes | Gilson | 10082012 | https://artic.network/ebov/ebov-seq-kit.html | 210 | 262.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 525.00 | |||||||||||||||
26 | Sprout Microfuge (12V) | PhilipHarris | B8R04574 | https://artic.network/ebov/ebov-seq-kit.html | 170 | 212.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 425.00 | |||||||||||||||
27 | PV-1 Mini Vortex | FisherSci | PV-1 | https://artic.network/ebov/ebov-seq-kit.html | 150 | 187.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 375.00 | |||||||||||||||
28 | Portable Heat Block | Mobitec | Pad3-H-CB | https://artic.network/ebov/ebov-seq-kit.html | 310 | 387.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 387.50 | |||||||||||||||
29 | DynaMag-2 magnetic rack | Thermo | 12321D | https://artic.network/ebov/ebov-seq-kit.html | 540 | 675 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 675.00 | |||||||||||||||
30 | MiniPCR™ Mini16 | Cambio | Mp-QP-1016-01 | https://artic.network/ebov/ebov-seq-kit.html | 675 | 843.75 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 1,687.50 | |||||||||||||||
31 | Quantus Fluorometer | Promega | E6150 | https://artic.network/ebov/ebov-seq-kit.html | 1,236 | 1545 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 1,545.00 | |||||||||||||||
32 | PCR Workstation | StarLabs | E2396-9693 | https://artic.network/ebov/ebov-seq-kit.html | 20 | 25 | https://artic.network/ebov/ebov-seq-kit.html | 3 | $ 75.00 | |||||||||||||||
33 | Collapsible Tube racks (large x5) | FisherSci | 11710634 | https://artic.network/ebov/ebov-seq-kit.html | 50 | 62.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 62.50 | |||||||||||||||
34 | Cold Racks (0.2-0.5mL) | Sigma | Z606634-1EA | https://artic.network/ebov/ebov-seq-kit.html | 50 | 62.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 125.00 | |||||||||||||||
35 | Lab Mats | StarLab | E3860-0061 | https://artic.network/ebov/ebov-seq-kit.html | 25 | 31.25 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 62.50 | |||||||||||||||
36 | USB Lights | Amazon | Jebsen-T05 | https://artic.network/ebov/ebov-seq-kit.html | 20 | 25 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 50.00 | |||||||||||||||
37 | Portable UV Lamps | Amazon | B078LSR3MG | https://artic.network/ebov/ebov-seq-kit.html | 20 | 25 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 50.00 | |||||||||||||||
38 | Portable glove box | Amazon | B01B1MGRR0 | https://artic.network/ebov/ebov-seq-kit.html | 70 | 87.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 175.00 | |||||||||||||||
39 | Biosafety cabinet | 1,430.00 | https://www.labx.com/item/cleatech-simple-closed-loop-glovebox-two-port-clear/714812 | 1 | $ 1,430.00 | |||||||||||||||||||
40 | Computer | 2000 | 1 | $ 2,000.00 | ||||||||||||||||||||
41 | ||||||||||||||||||||||||
42 | Consumables and Disposables | $ 2,206.25 | ||||||||||||||||||||||
43 | Stacked 1000µL Filter Tips (500) | FisherSci | 11977724 | https://artic.network/ebov/ebov-seq-kit.html | 140 | 175 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 350.00 | |||||||||||||||
44 | Stacked 100µL Filter Tips (1000) | FisherSci | 11947724 | https://artic.network/ebov/ebov-seq-kit.html | 60 | 75 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 75.00 | |||||||||||||||
45 | Stacked 10µL Filter Tips (1000) | FisherSci | 11907724 | https://artic.network/ebov/ebov-seq-kit.html | 60 | 75 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 75.00 | |||||||||||||||
46 | 1.5mL Eppendorf Tubes (500) | StarLab | I1415-2500 | https://artic.network/ebov/ebov-seq-kit.html | 40 | 50 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 100.00 | |||||||||||||||
47 | 8-strip PCR tubes with Individual Caps, 0.2 ml (1000) | StarLab | I1402-2900 | https://artic.network/ebov/ebov-seq-kit.html | 115 | 143.75 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 143.75 | |||||||||||||||
48 | 0.5 ml thin-walled tubes (1000) | Axygen | PCR-05-C | https://artic.network/ebov/ebov-seq-kit.html | 40 | 50 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 50.00 | |||||||||||||||
49 | MyGo PCR Tubes (500) | IT-IS | SKU7688 | https://artic.network/ebov/ebov-seq-kit.html | 85 | 106.25 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 106.25 | |||||||||||||||
50 | BioBIn® Disposal Bins (100) | FisherSci | 10394373 | https://artic.network/ebov/ebov-seq-kit.html | 121 | 151.25 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 151.25 | |||||||||||||||
51 | Gloves (S/M/L, 100) | StarLab | SG-P-X | https://artic.network/ebov/ebov-seq-kit.html | 10 | 12.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 25.00 | |||||||||||||||
52 | Paper Towels | Generic | https://artic.network/ebov/ebov-seq-kit.html | 0 | https://artic.network/ebov/ebov-seq-kit.html | 4 | $ - | |||||||||||||||||
53 | Decon Wipes (240) | MediPal | PMC6038 | https://artic.network/ebov/ebov-seq-kit.html | 12 | 15 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 30.00 | |||||||||||||||
54 | Back-tie Lab Gowns (45G) | VWR | 113-8316 | https://artic.network/ebov/ebov-seq-kit.html | 56 | 70 | https://artic.network/ebov/ebov-seq-kit.html | 10 | $ 700.00 | |||||||||||||||
55 | RNAse Away (250mL) | Thermo | 10328011 | https://artic.network/ebov/ebov-seq-kit.html | 120 | 150 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 300.00 | |||||||||||||||
56 | DNA Away (250mL) | Thermo | 7010PK | https://artic.network/ebov/ebov-seq-kit.html | 40 | 50 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 100.00 | |||||||||||||||
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58 | Cold Chain, Power and Storage | $ 3,357.50 | ||||||||||||||||||||||
59 | Flat dehydrated cold sheets (ThermaFreeze) | Amazon | B003ET2G74 | https://artic.network/ebov/ebov-seq-kit.html | 15 | 18.75 | https://artic.network/ebov/ebov-seq-kit.html | 10 | $ 187.50 | |||||||||||||||
60 | Thermos Radiance Cooler Bag | Amazon | B00BHW5B4U | https://artic.network/ebov/ebov-seq-kit.html | 10 | 12.5 | https://artic.network/ebov/ebov-seq-kit.html | 4 | $ 50.00 | |||||||||||||||
61 | Portable Fridge Bag | Amazon | B008N5UU3G | https://artic.network/ebov/ebov-seq-kit.html | 70 | 87.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 87.50 | |||||||||||||||
62 | Credo Cube Series 20M 4L | Pelican | Contact Supp. | https://artic.network/ebov/ebov-seq-kit.html | 0 | https://artic.network/ebov/ebov-seq-kit.html | $ - | |||||||||||||||||
63 | PowerAdd Pilot Pro2 85Wh LiION 9-20V | Amazon | B013HXKZYW | https://artic.network/ebov/ebov-seq-kit.html | 80 | 100 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 200.00 | |||||||||||||||
64 | Solar Charger, 85Wh, LiION 5-20V | Amazon | B00AE9FBCO | https://artic.network/ebov/ebov-seq-kit.html | 85 | 106.25 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 212.50 | |||||||||||||||
65 | Goal Zero Yeti 150 Portable Power Station | GoalZero | SKU22004 | https://artic.network/ebov/ebov-seq-kit.html | 200 | 250 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 250.00 | |||||||||||||||
66 | Goal Zero Nomad 28 Plus Solar Panel | Goal Zero | SKU11805 | https://artic.network/ebov/ebov-seq-kit.html | 250 | 312.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 312.50 | |||||||||||||||
67 | Surge Protector Extension (4-plug) | Belkin | B0002AG0F2 | https://artic.network/ebov/ebov-seq-kit.html | 6 | 7.5 | https://artic.network/ebov/ebov-seq-kit.html | 3 | $ 22.50 | |||||||||||||||
68 | SKROSS Universal Mini Travel Adaptor | Amazon | B00OHSWG6C | https://artic.network/ebov/ebov-seq-kit.html | 10 | 12.5 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 25.00 | |||||||||||||||
69 | Peli Air 1615 Waterproof Equipment Case | RS Comp. | 121-8893 | https://artic.network/ebov/ebov-seq-kit.html | 550 | 687.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 687.50 | |||||||||||||||
70 | Eagle Creek Cargo Hauler, 120L | Amazon | B01HIGO3OC | https://artic.network/ebov/ebov-seq-kit.html | 150 | 187.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 187.50 | |||||||||||||||
71 | BESTEK 200W Power Inverter DC 12V | Amazon | B01N2P9V64 | https://artic.network/ebov/ebov-seq-kit.html | 30 | 37.5 | https://artic.network/ebov/ebov-seq-kit.html | 1 | $ 37.50 | |||||||||||||||
72 | Samsung Portable SSD T5 1TB | Amazon | B074M774TW | https://artic.network/ebov/ebov-seq-kit.html | 320 | 400 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 800.00 | |||||||||||||||
73 | Seagate® 5TB External HD | Amazon | B01M058Q39 | https://artic.network/ebov/ebov-seq-kit.html | 119 | 148.75 | https://artic.network/ebov/ebov-seq-kit.html | 2 | $ 297.50 | |||||||||||||||
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