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CategoryDNBSEQ-G50 (config 1)DNBSEQ-G50 (config 2)MiniSeqDNBSEQ-G50 Advantages (Green)/Disadvantages (Red)
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Sequencing TechnologySequencing TechnologyDNBSEQ™ technology
1. DNA nanoballs are generated by linear rolling circle amplification (RCA). RCA avoids the exponential accumulation of errors, GC biases observed with other amplification methods, such as PCR.
2. Only one strand of the dsDNA is used for circularization as the subsequent sequencing template. This results in a significant reduction in duplication rate and more effective usage in data. As a result, it enables generation of more data with lower cost on MGI platform.
3. Without accumulation of PCR errors, index hopping rate can be reduced to 0.0001％-0.0004％ using single index.		Using bridge PCR to generate DNA clusters on flow cell.
1. bridge PCR amplification can produce exponential accumulation of errors.
2. Two identical clusters can be generated by the complementary strands of dsDNA library, increasing in duplication rate.
3. Using Examp technology, index hopping rate can reach 1%-6% with single index.		DNBSEQ™ technology with increased accuracy, decreased duplicates and reduced index hopping rate.
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FeaturesFlow cell/RUN11
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Lane/Flow cell11
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Effective ReadsFCS 100M, FCL 500MMid output kit 8M, high output kit 25M1. FCS can produce more data than MiniSeq.
2. FCL can be used for future throughput upgrade plan for customer.
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Read LengthSE50, SE100, PE100, PE150SE75, PE75, PE150More read length options for flexibility
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Flow cell TechnologyPatterned array
Optimized patterned array ensures that only one single DNB is attached at each spot, which results in better flow cell utilization with unprecedented uniformity. This enables a significant reduction in duplicate rate.		Random array of DNA clusters is prone to cross interference and low flow cell utilization.Patterned array can increase utilization without interference between DNBs.
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TATFCS SE100 ~10 hours
FCS PE100 ~30 hours
FCS PE150 ~43 hours		FCS SE100 ~10 hours
FCS PE100 ~20 hours
FCS PE150 ~28 hours		25M SE75 ~ 7 hours
25M PE75 ~ 13 hours
25M PE150 ~ 24 hours		TAT of SE sequencing is similar between two machines, but TAT of PE sequencing is longer for DNBSEQ-G50
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Detection Method2-color2-color
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Computer System & Operating EnvironmentComputer Hard Disk1.25TB4TB1TBMore storage space
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Computer OSWindows 10 64-bitWindows 7More updated operating system.
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Altitude<3000 m<3000 m<2000 mDNBSEQ-G50 can be used in higher altitude, especially in cities with altitude between 2000-3000m.
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PriceReagentList price
FCS PE100 800USD, 40USD/G
FCS PE150 980USD, 43USD/G (~75M)		Price with 30% off
25M SE75 1190 USD, 634 USD/G
25M PE75 1393 USD，371 USD/G
25M PE150 2226 USD，296 USD/G		DNBSEQ-G50 FCS reagent price or cost per G are both lower compared with 30% discount price of MiniSeq high output reagent.
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InstrumentList price: 146,000USDList price: 190,000 USDList price: 49,500 USDHigher instrument cost
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Application & SolutionWorkflowSupported by MGISP-100 automated library preparation system.
Advantages with MGISP-100: shorter TAT, higher efficiency, ease of use and less contamination.		Third-party instruments needed.Supported by MGI automated sample prep system.
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ApplicationKey applications：
Small whole genome sequencing, targeted gene sequencing (multiplex PCR- or hybridization-based), low-pass WGS.
Other applications：
RNA-Seq, WES, WGS		Key applications:
Small whole genome sequencing, targeted sequencing, gene expression profiling.
Other applications:
Small RNA-Seq, 16S
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SolutionMGI provides automated sample prep system MGISP-100, bioinformatics analysis accelerator MegaBOLT and laboratory information management system ZLIMS together with DNBSEQ-G50 to support a complete workflow from library prep to bioinformatic analysis.No total solutions.Total solutions from nucleic acid/DNA to report.
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SupportTechnical Support & After-Sales ServicesMultiple local technical support centers around the world provide timely and effective technical support and trainingMultiple local technical support centers around the world provide timely and effective technical support and training.
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IVD CertificateNMPA, CE-IVD certificatesNo IVD certificateMore IVD certificates
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