2015 MM1 recipes - MM1 MM1A M1AG

	B	C	D	E	F	G	H	I	J	K	L	M	N
1
2	MM1A
3	0.5	total L to prepare					example chemical	Location	bottle description
4
5	Steps:
6	1	Add desired H2O volume (millipore)
7			make sure ~20% of bottle volume is head space
8	2	Add a ~1 inch stir bar
9	3	Add the following compounds:
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11				state	mM
12		1.27	g K2HPO4	(anhydrous)	14.5			red cabinet on right	glass bottle on side
13		1.30	g NaH2PO4	(monohydrous)	18.8		Fischer S369-1	above balances
14		0.1	g MgSO4	(heptahydrous)	0.8		Sigma M1880-1kg	above balances
15		0.25	g (NH4)2SO4	(anhydrous)	3.8		Sigma A4418 - 1kg	above balances
16		7.5	g Bacto Agar				Ref # 214010	above balances
17
18	4	Remove stir bar (use larger stir bar)
19	5	Add autoclave tape w/ reminder to add trace elements
20	6	Autoclave
21	6	Add 0.5 mL of Viniac Trace Elements
22	7	Add pre-sterilized glucose if desired
23	8	Distribute into pre-sterilized bottles
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25										Glucose Solution
26	Glucose Solution										500	mL medium to add glucose to
27		1000	mL medium to add glucose to								0.5	wt% glucose desired
28		0.5	wt% glucose desired								2.5	g glucose
29		5	g glucose				Sigma G5400	above balances	round plastic		25	glucose stock wt %
30		25	glucose stock wt %			<-- move from 20% to 25% 2015/6/23					10	mL to dissolve in
31		20	mL to dissolve in							Autoclave
32	Autoclave
33											Molarity	0.02775372455	M
34												27.75372455	mM
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