my5C.xlsx

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1	PRIMER_NAME	REGION	TYPE	ASSEMBLY	CHROMOSOME	FRAGMENT_ID	PRIMER_ID	P_STARTPOS	P_ENDPOS	P_SPECIFIC	P_SPECIFIC_SIZE	P_FILLER	P_FILLER_SIZE	P_TM	P_GC	F_STARTPOS	F_ENDPOS	F_SIZE	ELEMENTID	INTERSECTIONID	E_NAME	P_MER	P_UBLAST	P_SBLAST	TAIL	PRIMER_SEQUENCE
2	5C_5184_PKD2-660kb-EcoR_FOR_8	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	8	1	88585940	88585969	CTCCCACTTCCACTGCAGTACCTCAGAA	28	AT	2	64.99	53.57	88580796	88585969	5173	'0'	'0'		200	1	22	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGATCTCCCACTTCCACTGCAGTACCTCAGAA
3	5C_5184_PKD2-660kb-EcoR_FOR_21	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	21	1	88643739	88643768	GCTTGAGAAATTTATAATGAGACTTGGGAA	30		0	57.74	33.33	88639527	88643768	4241	'0'	'0'		385	1	25	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGGCTTGAGAAATTTATAATGAGACTTGGGAA
4	5C_5184_PKD2-660kb-EcoR_FOR_25	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	25	1	88662194	88662223	ACAAATGATTAGTAGTGAGCATTGACTGAA	30		0	58.75	33.33	88650767	88662223	11456	'0'	'0'		88	1	12	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGACAAATGATTAGTAGTGAGCATTGACTGAA
5	5C_5184_PKD2-660kb-EcoR_FOR_50	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	50	1	88745210	88745239	AAGATAAGACCACCATTAATATGTACAGAA	30		0	56.2	30	88740973	88745239	4266	'0'	'0'		200	1	46	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGAAGATAAGACCACCATTAATATGTACAGAA
6	5C_5184_PKD2-660kb-EcoR_FOR_56	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	56	1	88759818	88759847	CTGTGTGGAAGAGCAGAGGGTAAACAGAA	29	C	1	63.64	48.28	88755141	88759847	4706	'0'	'0'		331	1	26	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGCCTGTGTGGAAGAGCAGAGGGTAAACAGAA
7	5C_5184_PKD2-660kb-EcoR_FOR_59	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	59	1	88763700	88763729	CTGATTTGGAACCCCACAAACAAAAGGGAA	30		0	63.61	43.33	88760732	88763729	2997	'0'	'0'		227	1	42	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGCTGATTTGGAACCCCACAAACAAAAGGGAA
8	5C_5184_PKD2-660kb-EcoR_FOR_62	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	62	1	88778062	88778091	TGAGAGAATCTGATGCATCCATTAGCTGAA	30		0	61.56	40	88769632	88778091	8459	'0'	'0'		167	1	12	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTGAGAGAATCTGATGCATCCATTAGCTGAA
9	5C_5184_PKD2-660kb-EcoR_FOR_65	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	65	1	88786205	88786234	ACTACTCCAGTCATGTTGATACCATCTGAA	30		0	60.89	40	88784994	88786234	1240	'0'	'0'		125	1	17	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGACTACTCCAGTCATGTTGATACCATCTGAA
10	5C_5184_PKD2-660kb-EcoR_FOR_69	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	69	1	88794870	88794899	GGAAACTTGTTGGTAAAAATCTTGAATGAA	30		0	57.26	30	88792940	88794899	1959	'0'	'0'		324	1	65	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGGGAAACTTGTTGGTAAAAATCTTGAATGAA
11	5C_5184_PKD2-660kb-EcoR_FOR_78	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	78	1	88819651	88819680	CGACAGAGCTACCAACATCCAAGGTGAA	28	GT	2	63.88	50	88813978	88819680	5702	'0'	'0'		95	1	7	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGGTCGACAGAGCTACCAACATCCAAGGTGAA
12	5C_5184_PKD2-660kb-EcoR_FOR_83	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	83	1	88838836	88838865	AGAAAGAACAATATGGAATGCATTCCAGAA	30		0	59.33	33.33	88836797	88838865	2068	'0'	'0'		257	1	25	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGAGAAAGAACAATATGGAATGCATTCCAGAA
13	5C_5184_PKD2-660kb-EcoR_FOR_93	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	93	1	88860759	88860788	TTTAGGTAGCAGTTTCCATAAAAAGCAGAA	30		0	59.17	33.33	88859401	88860788	1387	'0'	'0'		245	1	49	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTTTAGGTAGCAGTTTCCATAAAAAGCAGAA
14	5C_5184_PKD2-660kb-EcoR_FOR_102	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	102	1	88884674	88884703	TTTACTCCAACACTTAGCTTTCTTTTAGAA	30		0	57.13	30	88878075	88884703	6628	'0'	'0'		319	1	58	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTTTACTCCAACACTTAGCTTTCTTTTAGAA
15	5C_5184_PKD2-660kb-EcoR_FOR_109	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	109	1	88894569	88894598	GCATCCCAGACACATTCTTTACCCGTGAA	29	T	1	64.3	48.28	88893530	88894598	1068	'0'	'0'		146	1	4	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTGCATCCCAGACACATTCTTTACCCGTGAA
16	5C_5184_PKD2-660kb-EcoR_FOR_110	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	110	1	88895646	88895675	GACCCAGAGCTCTGCTATCCCTGAA	25	TTTTC	5	63.32	56	88894599	88895675	1076	'0'	'0'		124	1	15	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTTTTCGACCCAGAGCTCTGCTATCCCTGAA
17	5C_5184_PKD2-660kb-EcoR_FOR_115	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	115	1	88912702	88912731	ATACAGATCAGCTCACAAACATCAACTGAA	30		0	60.44	36.67	88905986	88912731	6745	'0'	'0'		181	1	25	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGATACAGATCAGCTCACAAACATCAACTGAA
18	5C_5184_PKD2-660kb-EcoR_FOR_123	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	123	1	88929633	88929662	CTATTTCCGGTACCCAGCGCGGAA	24	CGACGG	6	64.53	58.33	88925002	88929662	4660	'0'	'0'		16	1	2	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGCGACGGCTATTTCCGGTACCCAGCGCGGAA
19	5C_5184_PKD2-660kb-EcoR_FOR_124	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	124	1	88933622	88933651	ATCATACCAACACAGTCCGTGTATCCAGAA	30		0	62.93	43.33	88929663	88933651	3988	'0'	'0'		48	1	2	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGATCATACCAACACAGTCCGTGTATCCAGAA
20	5C_5184_PKD2-660kb-EcoR_FOR_125	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	125	1	88934369	88934398	GGTTGTGGTAAGGTTGTGCCAGTAACAGAA	30		0	64.31	46.67	88933652	88934398	746	'0'	'0'		89	1	16	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGGGTTGTGGTAAGGTTGTGCCAGTAACAGAA
21	5C_5184_PKD2-660kb-EcoR_FOR_128	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	128	1	88939338	88939367	ACGTATGATGCGTAACACAGTGTTTTTGAA	30		0	61.33	36.67	88938271	88939367	1096	'0'	'0'		115	1	33	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGACGTATGATGCGTAACACAGTGTTTTTGAA
22	5C_5184_PKD2-660kb-EcoR_FOR_130	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	130	1	88952960	88952989	TTTGATGTTCTGTTATTCAATACATAAGAA	30		0	54.05	23.33	88945197	88952989	7792	'0'	'0'		294	1	28	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTTTGATGTTCTGTTATTCAATACATAAGAA
23	5C_5184_PKD2-660kb-EcoR_FOR_138	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	138	1	88989261	88989290	GACCAGGCAGTTCCCTCATCTCTCTGAA	28	CT	2	64.89	53.57	88983649	88989290	5641	'0'	'0'		410	1	65	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGCTGACCAGGCAGTTCCCTCATCTCTCTGAA
24	5C_5184_PKD2-660kb-EcoR_FOR_152	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	152	1	89032093	89032122	CCACTGCTCTGAACATGGCTGGAA	24	TCTGAC	6	63.04	54.17	89027829	89032122	4293	'0'	'0'		236	1	28	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGTCTGACCCACTGCTCTGAACATGGCTGGAA
25	5C_5184_PKD2-660kb-EcoR_FOR_159	PKD2-660kb-EcoR	FOR	hg19_dna	chr4	159	1	89066197	89066226	GTAGAAGCCAAGATAGCCTCAAGTTGAGAA	30		0	61.77	43.33	89063894	89066226	2332	'0'	'0'		118	1	10	CCATCTCATCCCTGCGTGTCTCCGACTCAG	CCATCTCATCCCTGCGTGTCTCCGACTCAGGTAGAAGCCAAGATAGCCTCAAGTTGAGAA
26	5C_5184_PKD2-660kb-EcoR_REV_119	PKD2-660kb-EcoR	REV	hg19_dna	chr4	119	2	88918877	88918906	TTCTGCCAGCTCACTTTTCATTTTTCCATG	30		0	62.19	40	88918554	88918906	352	'0'	'0'		790	1	29	ATCACCGACTGCCCATAGAGAGG	TTCTGCCAGCTCACTTTTCATTTTTCCATGATCACCGACTGCCCATAGAGAGG
27	5C_5184_PKD2-660kb-EcoR_REV_121	PKD2-660kb-EcoR	REV	hg19_dna	chr4	121	2	88924590	88924619	TTCCTTCCTTTAATACCCATCTGAAGACCT	30		0	61.06	40	88920631	88924619	3988	'0'	'0'		216	1	41	ATCACCGACTGCCCATAGAGAGG	TTCCTTCCTTTAATACCCATCTGAAGACCTATCACCGACTGCCCATAGAGAGG
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