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1 | GOA-ON in a Box V2.2 Updated February 2025 | ||||||||||
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3 | Developed and published by Alexis Valauri-Orton and Dr. Kaitlyn Lowder at The Ocean Foundation, with support and methodology development from Dr. Christopher Sabine, Dr. Andrew Dickson, Dr. Sophie Chu, Dr. Kim Currie, the International Atomic Energy Agency, GOA-ON, and NOAA's Ocean Acidification Program. | ||||||||||
4 | If you are considering purchasing a kit, we recommend you obtain an editable version of this spreadsheet by going to File --> Make a Copy. Remove any items you already have access to and decide if your research question requires sensors or can be addressed through discrete samples alone. Customizing this list will reduce the overall price. | ||||||||||
5 | Price changes occur frequently. Please confirm prices and any bulk discounts with suppliers directly prior to defining budgets. Some supplies, such as the acids, may require hazardous materials shipping, which can increase prices. Additionally, these suppliers are chosen for US ordering and purchasers from other countries will likely benefit from finding domestic suppliers. | ||||||||||
6 | Item | Item Number/ Model | Vendor | Specifications | Use | Cost Per Unit | Rec quantity for kit | Rec backup quantity | Total cost per kit | Estimated packing & within-US shipping costs | Notes |
7 | Deployed Equipment | $8,644 | |||||||||
8 | iSAMI pH for the measurement of pH. Incl external battery housing and battery, cable, high-impact instrument case | N/A | Sunburst Sensors | Choice of pH sensor depends on data quality needs, budget, depth range, etc | Measuring spectrophotometric pH in situ | $5,295.00 | 1 | 0 | $5,295.00 | ||
9 | iSAMI maintenance kit | N/A | Sunburst Sensors | Recommended if purchasing an iSAMI to allow for in-house repairs | Repairing some common components of the iSAMI | $215.00 | 1 | 0 | $215.00 | ||
10 | 12V portable power supply | Powers iSAMI in the field before deployment | 1 | 0 | $8.59 | ||||||
11 | CTD-Diver, 10 meter depth | DI271 | Van Essen | To record salinity, temperature, and pressure at the site for carbonate chemistry calculations | $1,859.00 | 1 | 0 | $1,859.00 | |||
12 | CTD-Diver USB Reading Unit | AS330 | Van Essen | Required if CTD-Diver is purchased | To read CTD-Diver sensor | $225.00 | 1 | 0 | $225.00 | ||
13 | Garmin eTrex SE GPS | 010-00970-00 | Amazon | Mark sensor deployment location and to record sample collection site | $136.00 | 1 | 0 | $136.00 | |||
14 | Lenovo ThinkBook 14 Gen 4 AMD (14”; 512 GB storage, 16 GB ram), mouse, case, travel adapter | 21DH0013US | Lenovo | Sufficient storage and RAM for data analysis | Connect to sensors, upload data, write reports | $780.00 | 1 | 0 | $780.00 | Price fluctuates a lot depending on sales | |
15 | Microsoft Office Home & Student 2021 | B09H7GPR1G | Amazon | Other options possible for data analysis, eg Google Sheets | Data input and management through Excel | $124.99 | 1 | 0 | $124.99 | ||
16 | For Discrete Sampling | $3,218 | |||||||||
17 | Beta Plus Bottle Kit- 4.2L Horizontal, PVC | 3-1940-H62 | Wildco | At least 1 L to fill 2 250 mL bottles w/ overflow | Collects water samples at depth | $605.17 | 1 | 0 | $605.17 | ||
18 | Saint Gobain Performance Tygon tubing, 50 ft | 50-206-8946 | Fisher Scientific | ~1/4" inner diameter; lab-grade | Used to fill sample bottles without air introduction and for connecting ends of jacketed beaker | $57.84 | 1 | 0 | $57.84 | ||
19 | PYREX Reagent Bottles with Hollow Standard Taper Stoppers- 36/case | 02-940C | Fisher Scientific | 250 mL, 160 mm height, stopper number 19 (=plug seal) | Collecting and storing discrete samples | $825.88 | 2 | 0 | $1,651.76 | Fisher Scientific, $1560; Corning, $1142; Sigma Aldrich, $1150 ; Capital Scientific (on backorder), $1041 | |
20 | Mercury (II) chloride, 99.5%, ACROS Organics, 25 g | AA1227414 | Fisher Scientific | Desired purity may be considered | Poison sample so biological activity does not change the sample during storage | $37.93 | 1 | 0 | $37.93 | Fisher list price $48.44 | |
21 | 0.5 oz Clear LDPE Plastic Dropper Bottles, 50 count | 1201B07WHT | Amazon | Similiar item easily substituted | Dispensing mercuric chloride without spill risk | $14.99 | 1 | 0 | $14.99 | Berlin Packaging | |
22 | Apiezon L Grease, 50 g | 14-638-15B | Fisher Scientific | Only commonly-accepted grease currently | Applied to the stoppers to prevent any gas/seawater leakage | $180.10 | 1 | 0 | $180.10 | Fisher list price: $612. Other options: SupplyMyLab, $243 (3 in stock); HighVacDepot, $488 | |
23 | 10ml Syringe Sterile with Luer Slip Tip, 100 count | 638632928913 | Amazon | Dispenses grease easily and cleanly | $16.49 | 1 | 0 | $16.49 | Syringe 10mL with Luer connection' 6.2816.020; Metrohm | ||
24 | Acetal Copolymer Hose Clamps, 100 count | MFLX06832-20 | VWR | Internal diameter of 31-35 mm to match bottle neck diamter | Used with rubber bands to secure bottle stoppers | $58.10 | 1 | 0 | $58.10 | McMaster-Carr, $87 | |
25 | Natural Rubber Bands, 320 count | 12205T82 | McMaster-Carr | Thick enough to sit on bottle top | Secure bottle stoppers with hose clamps | $11.78 | 1 | 0 | $11.78 | ||
26 | Fluke 51 II Handheld Digital Probe Thermometer | 51-2 | Amazon | Measure sample temperature at time of collection & after TA titration | $381.09 | 1 | 0 | $381.09 | |||
27 | Fluke 80PK-22 SureGrip Immersion Temperature Probe | 80PK-22 | Amazon | Reliable calibration | Measure sample temperature at time of collection & after TA titration | $112.00 | 1 | 0 | $112.00 | Amazon $142 | |
28 | Alconox Powdered Precision Cleaner, 4 lb box | 1104-1 | Alconox | Cleans grease from sample bottles | $56.58 | 1 | 0 | $56.58 | |||
29 | Bottle-cleaning brushes, 8 count | N/A | Amazon | Fits in ~1 in diameter bottle neck | Cleans grease from sample bottles | $25.99 | 1 | 0 | $25.99 | ||
30 | Tube-cleaning brushes, 10 count | N/A | Amazon | Cleans grease or other debris | $7.90 | 1 | 0 | $7.90 | |||
31 | For pH Analysis | $9,719 | |||||||||
32 | Thermo Scientific Orion AquaMate 7100 Vis Spectrophotometer | 15-200-945 | Fisher Scientific | Measures pH spectrophotometrically | $5,399.65 | 1 | 0 | $5,399.65 | |||
33 | Thermo Scientific 10 mm square single-cell holder | AQX1SQVH | Fisher Scientific | Required to hold square cuvettes | $29.10 | 1 | 0 | $29.10 | |||
34 | Globe Scientific Spectrophotometer Cuvettes, 500 count | 21-200-258 | Fisher Scientific | Holds sample for measurement | $157.00 | 1 | 0 | $157.00 | Can replace with a few glass cuvettes to reduce small optical imperfections | ||
35 | Plastic 5 mL pasteur pipettes, 100 count | Amazon | Adds sample to cuvette | $14.99 | 1 | 0 | $14.99 | ||||
36 | Gilson PIPETMAN G Starter Kit | F167900G | Fisher Scientific | Dispenses sample | $1,879.00 | 1 | 0 | $1,879.00 | |||
37 | m-Cresol Purple sodium salt, 10 g | AC199250100 | Fisher Scientific | pH indicator dye | $89.05 | 1 | 0 | $89.05 | Fisher list price $108 | ||
38 | Hach Buffer Powder Pillows, pH 7.00, 50 ct | Fisher Scientific | Check performance of pH electrode, and use multimeter reading to help adjust pH of the dye solution | $45.94 | 1 | 0 | $45.94 | ||||
39 | Hach Buffer Powder Pillows, pH 10.01, 50 ct | Fisher Scientific | Check performance of pH electrode, and use multimeter reading to help adjust pH of the dye solution | $45.94 | 1 | 0 | $45.94 | ||||
40 | Sodium Hydroxide (White Pellets), Fisher BioReagents, 500g | BP359-500 | Fisher Scientific | Adjusts pH of dye | $33.24 | 1 | 0 | $33.24 | Fisher list price $77 | ||
41 | Hydrochloric Acid Solution, 2N (Certified), Fisher Chemical, 500 mL | SA431-500 | Fisher Scientific | Adjusts pH of dye | $26.05 | 1 | 0 | $26.05 | Fisher list price $106; other option | ||
42 | PerpHecT pH buffer packs, 7.00, 25 count | Fisher Scientific | Any calibration soln at 7.00 is fine | Check calibration of Metrohm pH electrode | $108.25 | 1 | 1 | $216.50 | Hach has powder version, but they can't be exported easily. Amazon carries them too. Hach Buffer Powder Pillows, pH 7.00, 50 ct | ||
43 | PerpHecT pH buffer packs, 10.01, 25 count | Fisher Scientific | Any calibration soln at 7.00 is fine | Check calibration of Metrohm pH electrode | $108.25 | 1 | 1 | $216.50 | Hach Buffer Powder Pillows, pH 10.01, 50 ct | ||
44 | OHAUS Ranger Count 3000 Counting Scale | 02-112-223 | Fisher Scientific | To weigh out m-cresol powder | $966.50 | 1 | 0 | $966.50 | |||
45 | Double-ended lab spatula, 3 count | Cole Parmer | Scoops powder onto weigh boat | $11.40 | 1 | 0 | $11.40 | $11.40 | Amazon option, 1 pack, $5.99 | ||
46 | Borosilicate funnel, 50 mm diameter, 2 count | Amazon | Facilitates addition of dye powder to volumetric flask | $7.30 | 1 | 0 | $7.30 | Cole Parmer option, 12 pk for $18.70 | |||
47 | Weigh boats, 20 mL, 100 count | Amazon | Weigh out indicator powder | $11.99 | 1 | 0 | $11.99 | Cole Parmer option, 100pk for $13.52 | |||
48 | 250 mL borosilicate Volumetric Flask with Ground Stopper, 2 count | Cole Parmer | Production of indicator dye | $15.70 | 1 | 0 | $15.70 | $15.70 | Amazon option, 2 count, $16.99 | ||
49 | 250 mL borosilicate media storage bottles, 2 count | Amazon | Storage for pH indicator dye | $122.84 | 1 | 0 | $122.84 | Cole Parmer option, $159.75 for 10 pk | |||
50 | Tris buffer in synthetic seawater, 125 mL | N/A | UCSD Scripps | Validates of spec pH measurements | $43.00 | 10 | 0 | $430.00 | $75.00 | ||
51 | For Alkalinity | $4,285 | |||||||||
52 | 34450 A Digital Multimeter, 5/12 Digit OLED Display | U3402A | Keysight Technologies | Displays volts during titration | $1,138.00 | 1 | 0 | $1,138.00 | Can also buy 3 on Newark for faster shipping | ||
53 | Multimeter powerline fuse, 0.25A | 2110-1533 | Keysight Technologies | 0.25A; 500V Time-Delay; 1.7-Ohm; 20X5.1mm | Backup in case multimeter fuse blows | $3.94 | 0 | 2 | $7.88 | ||
54 | Multimeter current input fuse, 0.4A | 2110-1503 | Keysight Technologies | 0.4A; 500 V fast acting; 32×6.3×6.3mm | Backup in case multimeter fuse blows | $7.09 | 0 | 2 | $14.18 | ||
55 | Multimeter current input fuse, 11 A | 2110-1402 | Keysight Technologies | 11A 1000V Fast-Acting 38X10-mm Cartridge | Backup in case multimeter fuse blows | $14.10 | 0 | 2 | $28.20 | ||
56 | Sensorex PHAMP-1 pH/ORP Preamplifier | PHAMP-1 | Amazon | Converts pH electrode signal into a stronger output for multimeter | $6.78 | 1 | 0 | $6.78 | |||
57 | BNC banana plug adaptor, 2 ct | OL1183-X | Amazon | Connects preamplifier to multimeter | $13.99 | 1 | 1 | $27.98 | |||
58 | LL-Ecotrode Plus pH electrode | 6.0262.100 | Metrohm | Measures pH during sample titration | $404.25 | 1 | 0 | $404.25 | |||
59 | Electrode cable 1m, BNC connection | 6.2104.090 | Metrohm | Connects pH probe to BNC adaptor | $185.85 | 1 | 0 | $185.85 | |||
60 | pH Electrode Maintenance Kit | 6.2325.000 | Metrohm | Maintenance for pH probe | $238.35 | 1 | 0 | $238.35 | |||
61 | Jacketed Beaker | 5340-03 | Ace Glass | Maintains sample temperature steadily | $142.86 | 2 | 0 | $285.72 | |||
62 | Thermo Scientific RT Basic Series Magnetic Stirrer- 2L with various plugs | 90-691-17 | Fisher Scientific | Establishes rapid acid reaction | $175.94 | 1 | 0 | $175.94 | Fisher list price $311 | ||
63 | Magnetic stir bar, 7x25mm, 10 count | 16-800-508 | Amazon | Establishes rapid acid reaction | $9.99 | 1 | 0 | $9.99 | Fisherbrand Polygon Stir Bar, $10 each | ||
64 | Magnetic Stir Bar Retriever, 250mm/10 inch | OSSTR250mm | Amazon | Safely removes stir bar from beaker | $13.99 | 1 | 0 | $13.99 | |||
65 | SP Bel-Art Roxy M Repeating Pipettor | F37310-0000 | Bel-Art | Dispenses at least 2 mL, has fine enough gradations and easy to dose accurately | Dispenses acid | $347.00 | 1 | 0 | $347.00 | This is a new replacement for the Gilmont Burette that is no longer manufactured, and it is not yet well-tested. Grainger option; sold on other sites as well. | |
66 | SP Bel-Art Roxy M Repeating Pipettor 2.5 mL tips, 100 pk | F37300-0003 | Bel-Art | $161.65 | 1 | 0 | $161.65 | ||||
67 | Fisherbrand Cast-Iron L-Shaped Base Support Stand | 11-474-207 | Fisher Scientific | Holds burette | $181.60 | 1 | 0 | $181.60 | |||
68 | Adjustable Prong Clamps | Amazon | Holds burette | $13.49 | 2 | 0 | $26.98 | Fisher Castaloy Adjustable Angle Clamps; 05-769Q; Fisher Scientific, $74.90 | |||
69 | Hydrochloric Acid, DILUT-IT Anlytical Concentrate, 1 count | 02-003-021 | Fisher Scientific | 0.1N HCl required for titration methodology | $51.60 | 1 | 0 | $51.60 | Option for 0.1N HCl required for titration methodology | ||
70 | Hydrochloric Acid, 1.00 N, Ricca Chemical, 1L | 370032 | Fisher Scientific | 0.1N HCl required for titration methodology | $32.51 | 1 | 0 | $32.51 | Fisher list price $47.90. Option for 0.1N HCl required for titration methodology | ||
71 | Calibrated alkalinity titrant, 1L | N/A | UCSD Scripps | 0.1N HCl required for titration methodology | $270.00 | 2 | 0 | $270.00 | $75 | Option for 0.1N HCl required for titration methodology | |
72 | PYREX Class A Volumetric Flask with PYREX Glass Standard Taper Stopper, 1L | 56401L | Fisher Scientific | Holds acid | $57.74 | 1 | 0 | $57.74 | Corning, $83 in packs of 6 = $497; Fisher list price $47.90 | ||
73 | Fisherbrand Reusable 50 mL Class A Volumetric Pipet | 13-650-2S | Fisher Scientific | Dispenses sample | $11.96 | 4 | 0 | $47.84 | Check stock on Corning version; Fisher list price $39 | ||
74 | Pipet bulb | 13-681-102 | Fisher Scientific | For use with glass pipets to measure alkalinity sample | $12.78 | 1 | 1 | $25.56 | Fisher list price 51.38 | ||
75 | CyberPower UPS System, 1000VA/600W | CP1000AVRLCD | Amazon | Must match voltage of country; if not, use a transformer as below | Provides backup power | $159.95 | 0 | 0 | $159.95 | ||
76 | Step-down transformer, Sevenstar AR-5000 Automatic Voltage Regulator OR 2000 Watt Diamond Series Voltage Transformer with Regulator & Stabilizer | AR-5000 | B&H photo supply or 220 Electronics | Provides stable power to multimeter and spectrophotometer | $155.00 | 1 | 0 | $155.00 | (cost is avg price between the two for calculations) | ||
77 | Adapter, Type B (US) to Type Needed Locally | Various | Amazon | Type B female to male type needed for country | Connects spec, laptop, scale, and stirrer to the wall | $9.00 | 4 | 0 | $36.00 | ||
78 | Adapter, Type C (EU) to Type Needed Locally | Various | Amazon | Ensure it can withstand voltage necessary | Connects transformer to the wall | $9.00 | 1 | 0 | $9.00 | ||
79 | Fisherbrand Traceable Salinity Meter Pen | 15-078-202 | Fisher Scientific | Accuracy <2% | Salinity measurements of discrete samples for calculations | $62.65 | 1 | 0 | $62.65 | Fisher list price, $122 | |
80 | Oakton Conductivity and TDS Standard, 80 mS; 500 mL | UX-00653-32 | Cole-Parmer | Calibration of higher range of salinity | $18.00 | 1 | 0 | $18.00 | Fisher Scientific, $28 | ||
81 | Oakton Conductivity and TDS Standard, 12,880 µS; 500 mL | EW-00606-10 | Cole-Parmer | Calibration at lower bound of salinity | $18.00 | 1 | 0 | $18.00 | Good for a year unopened, but prob okay for longer | ||
82 | Seawater reference materials for total alkalinity, 500 mL | N/A | UCSD Scripps | Validates TA measurements | $65.00 | 10 | 0 | $65.00 | $75 | ||
83 | Plastic Quick-Disconnect Tube Coupling for Air and Water- plug with shut-off | 5012K86 | McMaster-Carr | Can connect jacketed beaker to water bath if available | $9.30 | 0 | 0 | $9.30 | Only useful if user has a waterbath | ||
84 | Plastic Quick-Disconnect Tube Coupling for Air and Water- socket with shut-off | 5012K83 | McMaster-Carr | Can connect jacketed beaker to water bath if available | $12.33 | 0 | 0 | $12.33 | Only useful if user has a waterbath | ||
85 | Odds and ends | $141 | |||||||||
86 | Parafilm | 604945297190 | Amazon | Seal titration solution when not in use to reduce changes in concentration from evaporation | $30.70 | 1 | 0 | $30.70 | Fisher, $103 | ||
87 | Kimwipes, 3pk | 36000341553 | Amazon | Cleans any drops from bottle mouth before sealing and dries lab equipment | $6.38 | 1 | 0 | $6.38 | Price difference between Fisher + Amazon! ~$13 on Fisher and ~$5 on Amazon per box | ||
88 | Nitrile gloves | Amazon | Provides standard lab protection and cleanliness while handling samples | $9.99 | 1 | 1 | $19.98 | Order different sizes as needed | |||
89 | Cable/zip ties, variety pack | N/A | Amazon | Misc uses in the lab and field | $12.98 | 1 | 0 | $12.98 | |||
90 | Lab tape, 5 count | Amazon | Labels sample bottles and equipment | $21.89 | 1 | 0 | $21.89 | ||||
91 | LDPE Wash Bottle, 500 mL, 2 count | Amazon | Holds DI water to clean up at TA and spec pH | $10.99 | 1 | 0 | $10.99 | ||||
92 | Weatherproof notebook, 4 x 7" | No. 373 | Amazon | To record field and lab notes | $7.73 | 1 | 1 | $15.46 | |||
93 | Electrical tape | Amazon | Optional; wraps around iSAMI to make biofouling removal easier | $12.99 | 1 | 0 | $12.99 | Thicker option, $58; wider option, $28 | |||
94 | Copper tape | Amazon | Optional; wraps around iSAMI to reduce biofouling | $9.99 | 1 | 0 | $9.99 | ||||
95 | Mercury filtration | $293 | |||||||||
96 | Aries AF-10-3620 2.5'' x 10'' Mercury Selective Filter | AF-10-3620 | ServeAPure | Removes mercury from seawater samples that have been preserved | $83.39 | 2 | 0 | $166.78 | These should be changed annually | ||
97 | Spigot for self-supplied carboy/bucket, 3 count | N/A | Amazon | Spigot diameter should fit tubing below | Connects a bucket that can hold excess samples to run it to the filter | $8.99 | 1 | 0 | $8.99 | ||
98 | Mounting bracket for filter housing | 244047 | ServeAPure | Mounts filter housing to cabinet | $5.56 | 2 | 0 | $11.12 | |||
99 | Clear filter housing for 2.5x10" cartridges, 1/4" threaded ports | 158117 | ServeAPure | Holds mercury filters | $19.51 | 2 | 0 | $39.02 | |||
100 | Nipple 1/4" thread with o-rings | CP008 | Pure Water Products | Connects filter housings together | $3.00 | 2 | 0 | $6.00 | |||