Zhou Lab Plasmid List.xlsx
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pCDFduet-1StrepMCS1: ECFP-protein; MCS2: proteinBamHI, EcoRI; NdeI, XhoIJessica
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EYFP-pet15bTEVpet15b-HIS-TEVAmpHis10-TEV-EYFP-proteinXhoI, BamHIdigest inefficient, add extra enzyme and timeJessica
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NusApETM-60KanNusA-His6-TEV-proteinNcoI, BamHISu
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p21t10pET21bAmpprotein-TEV-His(10)full pET21 MCSHayleyYoung, et al. JBC 2013
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pACYC184Tet, CamJinshi
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pBAD33CamParab promoter, no RBStight expression controlJinshi
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pBAD33.1pBAD33.1Camadd RBSJinshi, Hak Suk
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pCDFduet-1pCDFduet-1StrepMCS1: His6-protein; MCS2: proteinBamHI, EcoRI; NdeI, XhoIBamHI out of frame with His6Jessica
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pDEST17pDEST17AmpHis(6)-gateway site-proteinGateway systemHayley
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pET15b-fusionproteinpET15bAmpprotein1-His6-TEV-protein2NcoI+XhoI, NdeI+BamHISu
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pET15b-His-GB1-TEVpET15bAmpHis10-GB1-TEV-ProteinNdeI, XhoI, BamHISu
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pET15b-His-MBP-TEVpET15bAmpHis10-GB1-TEV-ProteinNdeI, XhoI, BamHISu
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pET15b-HIS-TEVpET15bAmpHis10-TEV-proteinNdeI, XhoI, BamHISu
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pET15b-KSI-Flag-thrombin-HispET15bAmpKSI-Flag-thrombin-His8-proteinNdeI, XhoI, BamHISu
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pET15b-KSI-thrombin-HispET15bAmpKSI-thrombin-His8-proteinNdeI, XhoI, BamHISu
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pET15b-NoTEVpET15bAmpHis6-ProteinNdeI, BamHISu
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pET16b*-AasSpET16bAMPHis10-TEV-proteinAli
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pET16b*-ACP-ACPS (pET16b*-AM)pET16bAMPHis10-TEV-protein-RBS-proteinAliMasoudi, et al. Nature 2013
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pET16b*-Flag-ACP-ACPSpET16bAMPHis10-TEV-FLAG-protein-RBS-proteinAli
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pET21apET21aAmpprotein-His6full pET21 MCSSu
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pET26b-MBPpET26bKanHis10-MBP-PPX-ProteinAvrII, XhoISu
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pET26b-pelB-MBPpET26bKanpelB-His10-MBP-PPX-ProteinAvrII, XhoISu
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pET28a-MSPgenespET28aKanHis6-TEV-MSPgenesSu
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pET30a-GB1pET30aKanGB1-Protein-His6BamHI, XhoISu
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pET30a-His-GB1pET30aKanHis6-GB1-ProteinBamHI, EcoRISu
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pET32a-GSAPpET32aAmpTRX-His6-thrombin-Stag-enterokinase-proteinBamHI, HindIIISu
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pGAD424mutpGAD424AmpGAL4 Activation Domain - proteinBamHI, NdeI, EcoRI, XhoIY2H, updated cloning siteJessica
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pGBT9mutpGBT9AmpGAL4 DNA Binding Domain - proteinBamHI, NdeI, EcoRI, XhoIY2H, updated cloning siteJessica
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pGEX-His-NBpGEX-2TAmpGST-thrombin-His8-proteinNdeI, BamHISu
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pGEX2T-NBpGEX-2TAmpGST-thrombin-ProteinNdeI, BamHISu
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pHSCpET21bAmpprotein-TEV-His(10)MCS ends at HindIIIHayley
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pKD46AmpRed recombinase, temp sensitiveJinshi
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pMAK705CamPtac promoter, no RBStemp sensitiveJinshi
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pMALpMAL-c2KanHis6-MBP-TEV-proteinBamHI, EcoRISu
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pMAL-CBP-His-MBPpMAL-c2KanCBP-His6-MBP-TEV-proteinBamHI, EcoRISu
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pMAL-His-CBP-MBPpMAL-c2KanHis6-CBP-MBP-TEV-proteinBamHI, EcoRISu
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pMAL-His-MBP-CBPpMAL-c2KanHis6-MBP-TEV-protein-CBPBamHI, EcoRISu
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pMAL-PPXpMAL-c2KanHis6-MBP-PPX-proteinBamHI, EcoRISu
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pTrc-His-MBP-TEVpTrc-HisBAmppTrc-10His-MBP-TEV-proteinBamHI, KpnINing
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pTRC-His-TRX-thrombinpTRCAmpHis-TRX-thrombin-proteinBamHI, EcoRISu
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pTRC-KSI-His-thrombinpTRCAmpKSI-His6-thrombin-proteinBamHI, EcoRISu
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pTRC-KSI-thrombinpTRCAmpKSI-thrombin-proteinBamHI, EcoRISu
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pTRCHisA,B,CpTRCAmpHis-Proteinfull Trc MCSSu
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pTRXpTRXAmpTRX-His10-thrombin-ProteinNdeI, BamHI, EcoRI, XhoISu
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pWSK29AmpPtac promoter, no RBSlow copyJinshi
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Vector NameParent VectorAntibioticResulting ConstructRestriction Site/Cloning NotesOther NotesLab MemberCitation
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