ROME_screen_conditions

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB
1	Gel file name	Lane # on gel	Total # of lanes on gel	Buffer	Buffer Concentration	Buffer Unit	Salt	Salt Concentration	Salt Unit	Detergent	Detergent Concentration	Detergent Unit	Other	Other Concentration	Other Unit	pH	Mass Ladder	Quantity Std	Over-expressed	Protein Systematic Name	Tag Type	Tag Location	Antibody	Other Capture	Notes	Single Reagent Flag	Elution Method	Elution Reagent
2
3				NH4 Acet	500	mM	NaCl	250	mM	TRIT X100	1	%v/v				7
4				NH4 Acet	750	mM	NaCl	250	mM	TRIT X100	1	%v/v				7
5				HEPES-Na	20	mM	NaCl	300	mM	Tween 20	0.1	%v/v				7.4
6				HEPES-Na	20	mM	NaCl	500	mM	Tween 20	0.1	%v/v				7.4
7				HEPES-Na	20	mM	NaCl	300	mM	CHAPS	5	mM				7.4
8				HEPES-Na	20	mM	NaCl	500	mM	CHAPS	5	mM				7.4
9				HEPES-Na	20	mM	NaCl	500	mM	TRIT X100	1	%v/v				7.4
10				HEPES-Na	20	mM	NaCl	100	mM	Sarcosyl	1	mM				7.4
11				HEPES-Na	20	mM	NaCl	300	mM	Sarcosyl	1	mM				7.4
12				HEPES-Na	20	mM	MgCl2	50	mM	Tween 20	0.1	%v/v				7.4
13				HEPES-Na	20	mM	MgCl2	100	mM	Tween 20	0.1	%v/v				7.4
14				HEPES-Na	20	mM	MgCl2	100	mM	TRIT X100	1	%v/v				7.4
15				HEPES-K	20	mM	K Acet	300	mM	Tween 20	0.1	%v/v				7.4
16				HEPES-K	20	mM	K Acet	500	mM	Tween 20	0.1	%v/v				7.4
17				HEPES-K	20	mM	K Acet	500	mM	TRIT X100	1	%v/v				7.4
18				TRIS-Cl	20	mM	Na3 Cit	200	mM	TRIT X100	1	%v/v				8
19				TRIS-Cl	20	mM	Na3 Cit	300	mM	TRIT X100	1	%v/v				8
20				NaPhos	300	mM				TRIT X100	1	% v/v				8
21				NaPhos	500	mM				TRIT X100	1	% v/v				8
22				NaPhos	300	mM				Tween 20	0.1	% v/v				8
23				NaPhos	500	mM				Tween 20	0.1	% v/v				8
24				NaPhos	500	mM				TRIT X100	1	% v/v	glutaraldehyde	2	mM	8
25				NaPhos	500	mM				Tween 20	0.1	% v/v	glutaraldehyde	2	mM	8
26				HEPES-Na	20	mM	NaCl	500	mM	TRIT X100	1	%v/v	glutaraldehyde	2	mM	7.4
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100