| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | AA | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Cre Name | Sequence | Product size | Annealing Temp. | Cycles | MgCl2 | NOTE | Strain Name | PCR OK | Update record | ||||||||||||||||||
2 | Ai14 | oIMR9020 | 5'-AAGGGAGCTGCAGTGGAGTA-3' | Wild type Forward | WT: 297bp | 60°C | 28 | 1.5mM | B6.Cg-Gt(ROSA)26Sor <tm14(CAG-tdTomato)Hze>/J | O | First Upload: 2023.12.18 Iris | Finished (%) | 103.3333333 | |||||||||||||||
3 | oIMR9021 | 5'-CCGAAAATCTGTGGGAAGTC-3' | Wild type Reverse | |||||||||||||||||||||||||
4 | oIMR9103 | 5'-GGCATTAAAGCAGCGTATCC-3' | Mutant Reverse | Mut: 196bp | WPRE | 2023.12 Added nestin-cre line | Total | 38 | ||||||||||||||||||||
5 | oIMR9105 | 5'-CTGTTCCTGTACGGCATGG-3' | Mutant Forward | tdTomato | 2024.05.13 Iris | Actually used | 30 | *Deduct the Cre line with unknown purpose and no longer used. | ||||||||||||||||||||
6 | Ai213 | 22159 | 5'-GTGTAGCCCTGGCTTTTCTG-3' | Wild type Forward | WT: 224bp | 60°C | 28 | - | WT and Mut need to run PCR separately. | B6;129S6-Igs7<tm213(CAG-EGFP,CAG-mOrange2,CAG-mKate2)Tasic>/J | O | 2024.08.01 Iris | ||||||||||||||||
7 | 29830 | 5'-CTGGCAGTCCTTGAGTTCC-3' | Wild type Reverse | Run Ai213 protocol | 2024.08.15 Bella | |||||||||||||||||||||||
8 | 52755 | 5'-CCGCGTTTCTTCCTTTTCC-3' | Mutant Forward | Mut: 290bp | Mut-F primer 2x-3x | 2025.01.10 Iris | ||||||||||||||||||||||
9 | 53465 | 5'-CAGCTTAGCGGTCTGAAAGC-3' | Mutant Reverse | 2025.08.01 Katrina (Ai32) | ||||||||||||||||||||||||
10 | Ai32 | oIMR9020 | 5’-ACATGGTCCTGCTGGAGTTC-3’ | Mutant Forward | Mut: 212bp | If use 2% gel, run for 40~50mins to separate the band | O | 2025.12.29 Iris CB1-flox | ||||||||||||||||||||
11 | oIMR9021 | 5’-GGCATTAAAGCAGCGTATCC -3’ | Mutant Reverse | |||||||||||||||||||||||||
12 | oIMR9102 | 5’-AAGGGAGCTGCAGTGGAGTA-3’ | Wild Type Forward | WT: 297bp | ||||||||||||||||||||||||
13 | oIMR9102 | 5’-CCGAAAATCTGTGGGAAGTC-3’ | Wild Type Reverse | |||||||||||||||||||||||||
14 | ASIC1-cre | 5'-TCCTCTCCCAAACACACAC-3' | Left loxP-F | LloxP: 410bp | 60°C | 28 | 1.5mM | loxP L R should be done separately | B6.129-Asic1 <tm1Ccli> | O | ||||||||||||||||||
15 | 5'-GAGTTCCCTCCAGATGTGAG-3' | Left loxP-R | PCR Finished (O) | 31 | ||||||||||||||||||||||||
16 | 5'-AGGCCTGCAAACTGTCATCT-3' | Right loxP-F | RloxP: 406bp | loxP Left primer can add more. ex. 2μl/sample | PCR Undone | -1 | *Deduct the Cre line with unknown purpose and no longer used. | |||||||||||||||||||||
17 | 5'-GTTGCATCTTGAGCCTCCTC-3' | Right loxP-R | ||||||||||||||||||||||||||
18 | Crlr-cre | oIMR8744 | 5'-CAAATGTTGCTTGTCTGGTG-3' | Internal Positive Control Forward | IPcrtl: 200bp | 60°C | 28 | 1.5mM | Mossy cell / Crlr-cre / CA3-cre | Tg(Calcrl,cre)4688Nkza | O | Unknown purpose (Z) | 5 | |||||||||||||||
19 | oIMR8745 | 5'- GTCAGTCGAGTGCACAGTTT-3' | Internal Positive Control Reverse | No longer use (X) | 2 | |||||||||||||||||||||||
20 | 19157 | 5'-CGTCTACGGAGCGACAATTC-3' | Transgene Forward | TG: 375bp | ||||||||||||||||||||||||
21 | 19158 | 5'-TGCATCGACCGGTAATGC-3' | Transgene Reverse | |||||||||||||||||||||||||
22 | CB1-KO | 5'-GGATTCAGAATCATGAAGCACTCCA-3' | Wild type Reverse | WT: 301bp | 60°C | 28 | 1.5mM | Cnr1 <tm1Zim> | O | |||||||||||||||||||
23 | 5'-GTACCATCACCACAGACCTCCTC-3' | Common | Cre line 總數 | 38 | ||||||||||||||||||||||||
24 | 5'-AAGAACGAGATCAGCAGCCTCTGTT-3' | Mutant Reverse | 實際總數 | 30 | *扣除用途待確以及已不使用的Cre line | |||||||||||||||||||||||
25 | CB1-flox | 5'-GGAGCTGTCTCTGGTCCTCTT-3' | Forward | WT: 437 bp | 60°C | B6.129P2(Cg)-Cnr1tm1.2Ltz/J | ||||||||||||||||||||||
26 | 5'-AAACACCAGGCACCACAAAT-3' | Reverse | Mut: ~530 bp | |||||||||||||||||||||||||
27 | CCK | 11215 | 5'-GGGAGGCAGATAGGATCACA-3' | Wild type Forward | WT: 468bp | 60°C | 28 | 1.5mM | Mut primer needs to be twice as much as WT. | STOCK Cck <tm1.1(cre)Zjh>/J | O | |||||||||||||||||
28 | 11214 | 5'-GAGGGGTCGTATGTGTGGTT-3' | Common | 已跑過PCR的Cre line (O) | 31 | |||||||||||||||||||||||
29 | 9989 | 5'-TGGTTTGTCCAAACTCATCAA-3' | Mutant Forward | Mut: 215bp | 未跑PCR的Cre line | -1 | *扣除用途待確以及已不使用的Cre line | |||||||||||||||||||||
30 | cfos-tTA | oIMR0872 | 5'-AAGTTCATCTGCACCACCG-3' | Transgene | TG: 173bp | 60°C | 28 | 1.5mM | GFP | B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J | X | |||||||||||||||||
31 | oIMR1416 | 5'-TCCTTGAAGAAGATGGTGCG-3' | Transgene | GFP | 用途待確的Cre line (Z) | 5 | ||||||||||||||||||||||
32 | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPctrl: 324bp | O | 已不使用的Cre line (X) | 2 | |||||||||||||||||||||
33 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC -3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
34 | Mut-F (designed) | 5'-ATCATGGCCGACAAGCAGAA-3' | knock-in Forward | TG: 187bp | 1.5mM | self-designed primer from EGFP sequences; Run Ai213 protocol | O | |||||||||||||||||||||
35 | Mut-R (designed) | 5'-TCTCGTTGGGGTCTTTGCTC-3' | knock-in Reverse | self-designed primer from EGFP sequences | ||||||||||||||||||||||||
36 | CRH-cre | 10575 | 5'-CACGACCAGGCTGCGGCTAAC-3' | Wild type Forward | WT: 676bp | 55°C | 28 | 1.5mM | *Annealing Temp. = 55℃ | B6(Cg)-Crh <tm1(cre)Zjh>/J | O | |||||||||||||||||
37 | 10574 | 5'-CTTACACATTTCGTCCTAGCC-3' | Common | |||||||||||||||||||||||||
38 | 10576 | 5'-CAATGTATCTTATCATGTCTGGATCC-3' | Mutant Forward | Mut: 468bp | ||||||||||||||||||||||||
39 | Dlx5/6-Flpe | oIMR8744 | 5'-CAAATGTTGCTTGTCTGGTG-3' | Internal Positive Control Forward | IPctrl: 200bp | 60°C | 28 | 1.5mM | STOCK Tg(mI56i-flpe)39Fsh/J | O | ||||||||||||||||||
40 | oIMR8745 | 5'-GTCAGTCGAGTGCACAGTTT-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
41 | 9658 | 5'-CAGAATTGATCCTGGGGAGCTACG-3' | Transgene Forward | Trans: 406bp | ||||||||||||||||||||||||
42 | 9659 | 5'-CCAGGACCTTAGGTGGTGTTTTAC-3' | Transgene Reverse | |||||||||||||||||||||||||
43 | Drd2-cre | 5'-GTGCGTCAGCATTTGGAGCAA-3' | Drd2 (32108) F1 | TG: 700bp | 60°C | 28 | 1.5mM | B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd | O | |||||||||||||||||||
44 | 5'-CGGCAAACGGACAGAAGCATT-3' | CreGS R1 | ||||||||||||||||||||||||||
45 | Gad2-cre | 28223 | 5'-TCGTTGCACTGACGTGTTCT-3' | Wild type Forward | WT: 225bp | 60°C | 28 | 2.6mM | STOCK Gad2 <tm2(cre)Zjh>/J | O | ||||||||||||||||||
46 | 28224 | 5'-AACAGTTTGATGAGTGAGGTGA-3' | Common | |||||||||||||||||||||||||
47 | 19048 | 5'-CACTGCATTCTAGTTGTGGTTTG-3' | Mutant Forward | Mut: 176bp | ||||||||||||||||||||||||
48 | GAD67 | 5'-CCTTCTGTCTCCGAGACTTCGGTG-3' | Wild type Forward (Common) | 60°C | 30 | 1.5mM | GAD67-GFP-KI | ICR.Cg-Gad1<tm1.1Tama> | O | |||||||||||||||||||
49 | 5'-GCGCAGCCTCCCGCAGTGCCAGC-3' | Wild type Reverse | WT: 297bp | WT-R 0.5x, Mut-R 3x | ||||||||||||||||||||||||
50 | olMR1416 | 5'-TCCTTGAAGAAGATGGTGCG-3' | Mutant Reverse | KI: ca420bp | ||||||||||||||||||||||||
51 | Gli1 | oIMR7888 | 5'-GGGATCTGTGCCTGAAACTG-3' | Wild type Forward | WT: 136bp | 60°C | 28 | 2.6mM | Gli1-CreERT2 | STOCK Gli1<tm3(cre/ERT2)Alj>/J | O | |||||||||||||||||
52 | oIMR7889 | 5'-CTTGTGGTGGAGTCATTGGA-3' | Wild type Reverse | WT and Mut need to run PCR separately | ||||||||||||||||||||||||
53 | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Transgene Forward | Mut: 100bp | ||||||||||||||||||||||||
54 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Transgene Reverse | |||||||||||||||||||||||||
55 | Grik4-cre | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | WT: 324bp | 60°C | 28 | 2.6mM | C57BL/6-Tg(Grik4-cre)G32-4Stl/J | Z | ||||||||||||||||||
56 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
57 | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Transgene Forward | Mut: 100bp | ||||||||||||||||||||||||
58 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Transgene Reverse | |||||||||||||||||||||||||
59 | Jax-Cre | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Jax-CreF | 100bp | 60°C | 28 | 1.5mM | You can use; however, | For Cre | O | |||||||||||||||||
60 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Jax-CreR | Negative Control is necessary | ||||||||||||||||||||||||
61 | Klotho-flox | 5'-GTTATGTCTTGTTCCTGGCTTGTC-3' | Forward | WT: 282bp | 60°C | 28 | 1.5mM | Electrophoresis needs to run for more than 30 minutes (V=100). | O | |||||||||||||||||||
62 | 5'-CCATCTGTGGCTGTACGAGGAAT-3' | Reverse | Mut: 338bp | |||||||||||||||||||||||||
63 | Klotho-KO (x) | 0787-12 | 5'-GATGGGGTCGACGTCA-3' | Wild type | WT: 186bp | 65-55(↓1°C/cycle) | 40 | 1.7mM | B6;129S5-Kl <tm1Lex>/Mmucd | X | ||||||||||||||||||
64 | 0787-13 | 5'-TAAAGGAGGAAAGCCATTGTC-3' | Wild type | |||||||||||||||||||||||||
65 | Neo3a | 5'-GCAGCGCATCGCCTTCTATC-3' | Mutant | Mut: 455bp | ||||||||||||||||||||||||
66 | 0787-20 | 5'-ATGCTCCAGACATTCTCAGC-3' | Mutant | |||||||||||||||||||||||||
67 | mGluR5loxP | 28881 | 5'-GCTGAAAAGGACGAAACAACA-3' | Forward | WT: 210bp | 60°C | 28 | 1.5mM | B6.129-Grm5 <tm1.1Jixu>/J | O | ||||||||||||||||||
68 | 28882 | 5'-CGTTTTGAATCTTGGGGACA-3' | Reverse | Mut: 310bp | ||||||||||||||||||||||||
69 | Ndnf (x) | 26739 | 5'-ACCAGTGCCTAGGACCAGAC-3' | Wild type Forward | WT: 303bp | 60°C | 28 | 2.6mM | From Jackson Lab Unable to PCR DNA Discarded and not in use | Z | ||||||||||||||||||
70 | 47496 | 5'-GTACAACTTTTGTTTGTCACGAGAG-3' | Common | Need internal Positive Control | ||||||||||||||||||||||||
71 | 32628 | 5'-CTCATCAATGTATCTTATCATGTCTGG-3' | Mutant Forward | Mut: 199bp | ||||||||||||||||||||||||
72 | NDNF-cre | 5'- CACCCTGTTACGTATAGCCG-3' | NDNF-cre F | 330bp | 60°C | Ndnf <tm1.1(cre)Rudy>/J | O | |||||||||||||||||||||
73 | 5'- GAGTCATCCTTAGCGCCGTA-3' | NDNF-cre R | ||||||||||||||||||||||||||
74 | Nestin-cre | 5'-GCTAAACATGCTTCATCGTCGG-3 | 600-700bp | Nes-cre-5' | Z | |||||||||||||||||||||||
75 | 5'-GATCTCCGGTATTGAAACTCCAGC-3' | Nes-cre-3' | ||||||||||||||||||||||||||
76 | NG2creBAC | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPcrtl: 324bp | 60°C | 28 | 2.6mM | WT and Mut need to run PCR separately. | B6;FVB-lfi208 Tg(Cspg4-cre)1Akik/J | Z | |||||||||||||||||
77 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
78 | 30177 | 5'-AAATCTAAGCGCGGGTCTG-3' | Transgene Forward | TG: 425bp | ||||||||||||||||||||||||
79 | oIMR1709 | 5'-GCAAACGGACAGAAGCATTT-3' | Transgene Reverse | |||||||||||||||||||||||||
80 | NG2DsRedBAC(x) | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPcrtl: 324bp | 60°C | 28 | 2.6mM | STOCK Tg(Cspg4-DsRed.T1)1Akik/J | Z | ||||||||||||||||||
81 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
82 | oIMR8699 | 5'-TTCCTTCGCCTTACAAGTCC-3' | Transgene Forward | TG: 280bp | Cspg4 | |||||||||||||||||||||||
83 | oIMR8700 | 5'-GAGCCGTACTGGAACTGG-3' | Transgene Reverse | DsRed | ||||||||||||||||||||||||
84 | Osr1-flox | 5'-AAACCTGCTGGGCTTCTATG-3' | Forward TT1 | Osr1 <tm2.2Jian> | O | |||||||||||||||||||||||
85 | 5'-TGGGGTTAGTGGGGATAAGA-3' | Reverse TT2 | ||||||||||||||||||||||||||
86 | Prkcd-cre | 11559-tgF | 5'-GCTACATCAAGGCCATCGAC-3' | Transgene Forward | TG: 1kb | 65-55(↓1°C/cycle) | 40 | 1.5mM | STOCK Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat/Mmucd | O | ||||||||||||||||||
87 | 11559-tgR | 5'-AACTCCAGCAGGACCATGTGATCG-3' | Transgene Reverse | |||||||||||||||||||||||||
88 | PV | oIMR8290 | 5'-CAGAGCAGGCATGGTGACTA-3' | Wild type Forward | WT: 500bp | 60°C | 28 | 2.6mM | B6;129P2-Pvalb <tm1(cre)Arbr>/J | O | ||||||||||||||||||
89 | oIMR8291 | 5'-AGTACCAAGCAGGCAGGAGA-3' | Wild type Reverse | |||||||||||||||||||||||||
90 | 17283 | 5'-AAATGCTTCTGTCCGTTTGC-3' | Mutant Forward (Cre-F) | Mut: 163bp | ||||||||||||||||||||||||
91 | oIMR9377 | 5'-ATGTTTAGCTGGCCCAAATG-3' | Mutant Reverse (Cre-R) | |||||||||||||||||||||||||
92 | RiboTag | 9508 | 5'-GGGAGGCTTGCTGGATATG-3' | Forward | WT: 243bp | 60°C | 28 | 2.6mM | B6N.129-Rpl22 <tm1.1Psam>/J | O | ||||||||||||||||||
93 | 9509 | 5'-TTTCCAGACACAGGCTAAGTACAC-3' | Reverse | Mut: 290bp | ||||||||||||||||||||||||
94 | Sncg-flpo | 5'-CATTTCTTGGTCCTGGGAGA-3' | Wild type Forward | WT: 317bp | 60°C | 28 | 1.5mM | B6;129S6-Sncg<em1(flpo)Tasic>/J | O | |||||||||||||||||||
95 | 5'-TCAGTGTGCCAGGTTGAGAC-3' | Common | Mut: 229bp | |||||||||||||||||||||||||
96 | 5'-GCCTGAAGAACGAGATCAGC-3' | Mutant Forward | ||||||||||||||||||||||||||
97 | SOM/SST-Cre | 53429 | 5'-GAGGTCTGCCAACTCGAAC-3' | Wild type Forward | WT: 257bp | 60°C | 28 | 2.6mM | STOCK Sst <tm2.1(cre)Zjh>/J | O | ||||||||||||||||||
98 | 53430 | 5'-AGTCAAACGCTTGCTCTTCA-3' | Common | |||||||||||||||||||||||||
99 | 53428 | 5'-TCAGGTACATGGATCCACTAGTTCT-3' | Mutant Forward | Mut: 198bp | ||||||||||||||||||||||||
100 | SPAK-KO(flox) | 5'-GCCAGCACACAACATCTGTC-3' | Forward TT5 | Use freshly prepared primers every time | Stk39 <tm1.1Slin> | O | ||||||||||||||||||||||