| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | AA | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Cre Name | Sequence | Product size | Annealing Temp. | Cycles | MgCl2 | NOTE | Strain Name | PCR OK | Update record | ||||||||||||||||||
2 | Ai14 | oIMR9020 | 5'-AAGGGAGCTGCAGTGGAGTA-3' | Wild type Forward | WT: 297bp | 60°C | 28 | 1.5mM | B6.Cg-Gt(ROSA)26Sor <tm14(CAG-tdTomato)Hze>/J | O | First Upload: 2023.12.18 Iris | Finished (%) | 100 | |||||||||||||||
3 | oIMR9021 | 5'-CCGAAAATCTGTGGGAAGTC-3' | Wild type Reverse | |||||||||||||||||||||||||
4 | oIMR9103 | 5'-GGCATTAAAGCAGCGTATCC-3' | Mutant Reverse | Mut: 196bp | WPRE | 2023.12 Added nestin-cre line | Total | 39 | ||||||||||||||||||||
5 | oIMR9105 | 5'-CTGTTCCTGTACGGCATGG-3' | Mutant Forward | tdTomato | 2024.05.13 Iris | Actually used | 31 | *Deduct the Cre line with unknown purpose and no longer used. | ||||||||||||||||||||
6 | Ai213 | 22159 | 5'-GTGTAGCCCTGGCTTTTCTG-3' | Wild type Forward | WT: 224bp | 60°C | 28 | - | WT and Mut need to run PCR separately. | B6;129S6-Igs7<tm213(CAG-EGFP,CAG-mOrange2,CAG-mKate2)Tasic>/J | O | 2024.08.01 Iris | ||||||||||||||||
7 | 29830 | 5'-CTGGCAGTCCTTGAGTTCC-3' | Wild type Reverse | Run Ai213 protocol | 2024.08.15 Bella | |||||||||||||||||||||||
8 | 52755 | 5'-CCGCGTTTCTTCCTTTTCC-3' | Mutant Forward | Mut: 290bp | Mut-F primer 2x-3x | 2025.01.10 Iris | ||||||||||||||||||||||
9 | 53465 | 5'-CAGCTTAGCGGTCTGAAAGC-3' | Mutant Reverse | 2025.08.01 Katrina (Ai32) | ||||||||||||||||||||||||
10 | Ai32 | oIMR9020 | 5’-ACATGGTCCTGCTGGAGTTC-3’ | Mutant Forward | Mut: 212bp | If use 2% gel, run for 40~50mins to separate the band | O | 2025.12.29 Iris CB1-flox | ||||||||||||||||||||
11 | oIMR9021 | 5’-GGCATTAAAGCAGCGTATCC -3’ | Mutant Reverse | |||||||||||||||||||||||||
12 | oIMR9102 | 5’-AAGGGAGCTGCAGTGGAGTA-3’ | Wild Type Forward | WT: 297bp | ||||||||||||||||||||||||
13 | oIMR9102 | 5’-CCGAAAATCTGTGGGAAGTC-3’ | Wild Type Reverse | |||||||||||||||||||||||||
14 | Aldh1l1-cre | 21238 | 5'-CTGTCCCTGTATGCCTCTGG-3' | Internal Positive Control Forward | IPC: 415bp | 63℃ | 28 | 1.5% gel | ||||||||||||||||||||
15 | 21239 | 5'-AGATGGAGAAAGGACTAGGCTACA-3' | Internal Positive Control Reverse | Band of Mut is very light (blurry) | ||||||||||||||||||||||||
16 | 30308 | 5'-CTTCAACAGGTGCCTTCCA-3' | Transgene Forward | Mut: 198bp | ||||||||||||||||||||||||
17 | 31091 | 5'-GGCAAACGGACAGAAGCA-3' | Transgene Reverse | |||||||||||||||||||||||||
18 | ASIC1-cre | 5'-TCCTCTCCCAAACACACAC-3' | Left loxP-F | LloxP: 410bp | 60°C | 28 | 1.5mM | loxP L R should be done separately | B6.129-Asic1 <tm1Ccli> | O | ||||||||||||||||||
19 | 5'-GAGTTCCCTCCAGATGTGAG-3' | Left loxP-R | PCR Finished (O) | 31 | ||||||||||||||||||||||||
20 | 5'-AGGCCTGCAAACTGTCATCT-3' | Right loxP-F | RloxP: 406bp | loxP Left primer can add more. ex. 2μl/sample | PCR Undone | 0 | *Deduct the Cre line with unknown purpose and no longer used. | |||||||||||||||||||||
21 | 5'-GTTGCATCTTGAGCCTCCTC-3' | Right loxP-R | ||||||||||||||||||||||||||
22 | Crlr-cre | oIMR8744 | 5'-CAAATGTTGCTTGTCTGGTG-3' | Internal Positive Control Forward | IPcrtl: 200bp | 60°C | 28 | 1.5mM | Mossy cell / Crlr-cre / CA3-cre | Tg(Calcrl,cre)4688Nkza | O | Unknown purpose (Z) | 5 | |||||||||||||||
23 | oIMR8745 | 5'- GTCAGTCGAGTGCACAGTTT-3' | Internal Positive Control Reverse | No longer use (X) | 2 | |||||||||||||||||||||||
24 | 19157 | 5'-CGTCTACGGAGCGACAATTC-3' | Transgene Forward | TG: 375bp | ||||||||||||||||||||||||
25 | 19158 | 5'-TGCATCGACCGGTAATGC-3' | Transgene Reverse | |||||||||||||||||||||||||
26 | CB1-KO | 5'-GGATTCAGAATCATGAAGCACTCCA-3' | Wild type Reverse | WT: 301bp | 60°C | 28 | 1.5mM | Cnr1 <tm1Zim> | O | |||||||||||||||||||
27 | 5'-GTACCATCACCACAGACCTCCTC-3' | Common | Cre line 總數 | 39 | ||||||||||||||||||||||||
28 | 5'-AAGAACGAGATCAGCAGCCTCTGTT-3' | Mutant Reverse | 實際總數 | 31 | *扣除用途待確以及已不使用的Cre line | |||||||||||||||||||||||
29 | CB1-flox | 5'-GGAGCTGTCTCTGGTCCTCTT-3' | Forward | WT: 437 bp | 60°C | B6.129P2(Cg)-Cnr1tm1.2Ltz/J | ||||||||||||||||||||||
30 | 5'-AAACACCAGGCACCACAAAT-3' | Reverse | Mut: ~530 bp | |||||||||||||||||||||||||
31 | CCK | 11215 | 5'-GGGAGGCAGATAGGATCACA-3' | Wild type Forward | WT: 468bp | 60°C | 28 | 1.5mM | Mut primer needs to be twice as much as WT. | STOCK Cck <tm1.1(cre)Zjh>/J | O | |||||||||||||||||
32 | 11214 | 5'-GAGGGGTCGTATGTGTGGTT-3' | Common | 已跑過PCR的Cre line (O) | 31 | |||||||||||||||||||||||
33 | 9989 | 5'-TGGTTTGTCCAAACTCATCAA-3' | Mutant Forward | Mut: 215bp | 未跑PCR的Cre line | 0 | *扣除用途待確以及已不使用的Cre line | |||||||||||||||||||||
34 | cfos-tTA | oIMR0872 | 5'-AAGTTCATCTGCACCACCG-3' | Transgene | TG: 173bp | 60°C | 28 | 1.5mM | GFP | B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J | X | |||||||||||||||||
35 | oIMR1416 | 5'-TCCTTGAAGAAGATGGTGCG-3' | Transgene | GFP | 用途待確的Cre line (Z) | 5 | ||||||||||||||||||||||
36 | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPctrl: 324bp | O | 已不使用的Cre line (X) | 2 | |||||||||||||||||||||
37 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC -3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
38 | Mut-F (designed) | 5'-ATCATGGCCGACAAGCAGAA-3' | knock-in Forward | TG: 187bp | 1.5mM | self-designed primer from EGFP sequences; Run Ai213 protocol | O | |||||||||||||||||||||
39 | Mut-R (designed) | 5'-TCTCGTTGGGGTCTTTGCTC-3' | knock-in Reverse | self-designed primer from EGFP sequences | ||||||||||||||||||||||||
40 | CRH-cre | 10575 | 5'-CACGACCAGGCTGCGGCTAAC-3' | Wild type Forward | WT: 676bp | 55°C | 28 | 1.5mM | *Annealing Temp. = 55℃ | B6(Cg)-Crh <tm1(cre)Zjh>/J | O | |||||||||||||||||
41 | 10574 | 5'-CTTACACATTTCGTCCTAGCC-3' | Common | |||||||||||||||||||||||||
42 | 10576 | 5'-CAATGTATCTTATCATGTCTGGATCC-3' | Mutant Forward | Mut: 468bp | ||||||||||||||||||||||||
43 | Dlx5/6-Flpe | oIMR8744 | 5'-CAAATGTTGCTTGTCTGGTG-3' | Internal Positive Control Forward | IPctrl: 200bp | 60°C | 28 | 1.5mM | STOCK Tg(mI56i-flpe)39Fsh/J | O | ||||||||||||||||||
44 | oIMR8745 | 5'-GTCAGTCGAGTGCACAGTTT-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
45 | 9658 | 5'-CAGAATTGATCCTGGGGAGCTACG-3' | Transgene Forward | Trans: 406bp | ||||||||||||||||||||||||
46 | 9659 | 5'-CCAGGACCTTAGGTGGTGTTTTAC-3' | Transgene Reverse | |||||||||||||||||||||||||
47 | Drd2-cre | 5'-GTGCGTCAGCATTTGGAGCAA-3' | Drd2 (32108) F1 | TG: 700bp | 60°C | 28 | 1.5mM | B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd | O | |||||||||||||||||||
48 | 5'-CGGCAAACGGACAGAAGCATT-3' | CreGS R1 | ||||||||||||||||||||||||||
49 | Gad2-cre | 28223 | 5'-TCGTTGCACTGACGTGTTCT-3' | Wild type Forward | WT: 225bp | 60°C | 28 | 2.6mM | STOCK Gad2 <tm2(cre)Zjh>/J | O | ||||||||||||||||||
50 | 28224 | 5'-AACAGTTTGATGAGTGAGGTGA-3' | Common | |||||||||||||||||||||||||
51 | 19048 | 5'-CACTGCATTCTAGTTGTGGTTTG-3' | Mutant Forward | Mut: 176bp | ||||||||||||||||||||||||
52 | GAD67 | 5'-CCTTCTGTCTCCGAGACTTCGGTG-3' | Wild type Forward (Common) | 60°C | 30 | 1.5mM | GAD67-GFP-KI | ICR.Cg-Gad1<tm1.1Tama> | O | |||||||||||||||||||
53 | 5'-GCGCAGCCTCCCGCAGTGCCAGC-3' | Wild type Reverse | WT: 297bp | WT-R 0.5x, Mut-R 3x | ||||||||||||||||||||||||
54 | olMR1416 | 5'-TCCTTGAAGAAGATGGTGCG-3' | Mutant Reverse | KI: ca420bp | ||||||||||||||||||||||||
55 | Gli1 | oIMR7888 | 5'-GGGATCTGTGCCTGAAACTG-3' | Wild type Forward | WT: 136bp | 60°C | 28 | 2.6mM | Gli1-CreERT2 | STOCK Gli1<tm3(cre/ERT2)Alj>/J | O | |||||||||||||||||
56 | oIMR7889 | 5'-CTTGTGGTGGAGTCATTGGA-3' | Wild type Reverse | WT and Mut need to run PCR separately | ||||||||||||||||||||||||
57 | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Transgene Forward | Mut: 100bp | ||||||||||||||||||||||||
58 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Transgene Reverse | |||||||||||||||||||||||||
59 | Grik4-cre | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | WT: 324bp | 60°C | 28 | 2.6mM | C57BL/6-Tg(Grik4-cre)G32-4Stl/J | Z | ||||||||||||||||||
60 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
61 | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Transgene Forward | Mut: 100bp | ||||||||||||||||||||||||
62 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Transgene Reverse | |||||||||||||||||||||||||
63 | Jax-Cre | oIMR1084 | 5'-GCGGTCTGGCAGTAAAAACTATC-3' | Jax-CreF | 100bp | 60°C | 28 | 1.5mM | You can use; however, | For Cre | O | |||||||||||||||||
64 | oIMR1085 | 5'-GTGAAACAGCATTGCTGTCACTT-3' | Jax-CreR | Negative Control is necessary | ||||||||||||||||||||||||
65 | Klotho-flox | 5'-GTTATGTCTTGTTCCTGGCTTGTC-3' | Forward | WT: 282bp | 60°C | 28 | 1.5mM | Electrophoresis needs to run for more than 30 minutes (V=100). | O | |||||||||||||||||||
66 | 5'-CCATCTGTGGCTGTACGAGGAAT-3' | Reverse | Mut: 338bp | |||||||||||||||||||||||||
67 | Klotho-KO (x) | 0787-12 | 5'-GATGGGGTCGACGTCA-3' | Wild type | WT: 186bp | 65-55(↓1°C/cycle) | 40 | 1.7mM | B6;129S5-Kl <tm1Lex>/Mmucd | X | ||||||||||||||||||
68 | 0787-13 | 5'-TAAAGGAGGAAAGCCATTGTC-3' | Wild type | |||||||||||||||||||||||||
69 | Neo3a | 5'-GCAGCGCATCGCCTTCTATC-3' | Mutant | Mut: 455bp | ||||||||||||||||||||||||
70 | 0787-20 | 5'-ATGCTCCAGACATTCTCAGC-3' | Mutant | |||||||||||||||||||||||||
71 | mGluR5loxP | 28881 | 5'-GCTGAAAAGGACGAAACAACA-3' | Forward | WT: 210bp | 60°C | 28 | 1.5mM | B6.129-Grm5 <tm1.1Jixu>/J | O | ||||||||||||||||||
72 | 28882 | 5'-CGTTTTGAATCTTGGGGACA-3' | Reverse | Mut: 310bp | ||||||||||||||||||||||||
73 | Ndnf (x) | 26739 | 5'-ACCAGTGCCTAGGACCAGAC-3' | Wild type Forward | WT: 303bp | 60°C | 28 | 2.6mM | From Jackson Lab Unable to PCR DNA Discarded and not in use | Z | ||||||||||||||||||
74 | 47496 | 5'-GTACAACTTTTGTTTGTCACGAGAG-3' | Common | Need internal Positive Control | ||||||||||||||||||||||||
75 | 32628 | 5'-CTCATCAATGTATCTTATCATGTCTGG-3' | Mutant Forward | Mut: 199bp | ||||||||||||||||||||||||
76 | NDNF-cre | 5'- CACCCTGTTACGTATAGCCG-3' | NDNF-cre F | 330bp | 60°C | Ndnf <tm1.1(cre)Rudy>/J | O | |||||||||||||||||||||
77 | 5'- GAGTCATCCTTAGCGCCGTA-3' | NDNF-cre R | ||||||||||||||||||||||||||
78 | Nestin-cre | 5'-GCTAAACATGCTTCATCGTCGG-3 | 600-700bp | Nes-cre-5' | Z | |||||||||||||||||||||||
79 | 5'-GATCTCCGGTATTGAAACTCCAGC-3' | Nes-cre-3' | ||||||||||||||||||||||||||
80 | NG2creBAC | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPcrtl: 324bp | 60°C | 28 | 2.6mM | WT and Mut need to run PCR separately. | B6;FVB-lfi208 Tg(Cspg4-cre)1Akik/J | Z | |||||||||||||||||
81 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
82 | 30177 | 5'-AAATCTAAGCGCGGGTCTG-3' | Transgene Forward | TG: 425bp | ||||||||||||||||||||||||
83 | oIMR1709 | 5'-GCAAACGGACAGAAGCATTT-3' | Transgene Reverse | |||||||||||||||||||||||||
84 | NG2DsRedBAC(x) | oIMR7338 | 5'-CTAGGCCACAGAATTGAAAGATCT-3' | Internal Positive Control Forward | IPcrtl: 324bp | 60°C | 28 | 2.6mM | STOCK Tg(Cspg4-DsRed.T1)1Akik/J | Z | ||||||||||||||||||
85 | oIMR7339 | 5'-GTAGGTGGAAATTCTAGCATCATCC-3' | Internal Positive Control Reverse | |||||||||||||||||||||||||
86 | oIMR8699 | 5'-TTCCTTCGCCTTACAAGTCC-3' | Transgene Forward | TG: 280bp | Cspg4 | |||||||||||||||||||||||
87 | oIMR8700 | 5'-GAGCCGTACTGGAACTGG-3' | Transgene Reverse | DsRed | ||||||||||||||||||||||||
88 | Osr1-flox | 5'-AAACCTGCTGGGCTTCTATG-3' | Forward TT1 | Osr1 <tm2.2Jian> | O | |||||||||||||||||||||||
89 | 5'-TGGGGTTAGTGGGGATAAGA-3' | Reverse TT2 | ||||||||||||||||||||||||||
90 | Prkcd-cre | 11559-tgF | 5'-GCTACATCAAGGCCATCGAC-3' | Transgene Forward | TG: 1kb | 65-55(↓1°C/cycle) | 40 | 1.5mM | STOCK Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat/Mmucd | O | ||||||||||||||||||
91 | 11559-tgR | 5'-AACTCCAGCAGGACCATGTGATCG-3' | Transgene Reverse | |||||||||||||||||||||||||
92 | PV | oIMR8290 | 5'-CAGAGCAGGCATGGTGACTA-3' | Wild type Forward | WT: 500bp | 60°C | 28 | 2.6mM | B6;129P2-Pvalb <tm1(cre)Arbr>/J | O | ||||||||||||||||||
93 | oIMR8291 | 5'-AGTACCAAGCAGGCAGGAGA-3' | Wild type Reverse | |||||||||||||||||||||||||
94 | 17283 | 5'-AAATGCTTCTGTCCGTTTGC-3' | Mutant Forward (Cre-F) | Mut: 163bp | ||||||||||||||||||||||||
95 | oIMR9377 | 5'-ATGTTTAGCTGGCCCAAATG-3' | Mutant Reverse (Cre-R) | |||||||||||||||||||||||||
96 | RiboTag | 9508 | 5'-GGGAGGCTTGCTGGATATG-3' | Forward | WT: 243bp | 60°C | 28 | 2.6mM | B6N.129-Rpl22 <tm1.1Psam>/J | O | ||||||||||||||||||
97 | 9509 | 5'-TTTCCAGACACAGGCTAAGTACAC-3' | Reverse | Mut: 290bp | ||||||||||||||||||||||||
98 | Sncg-flpo | 5'-CATTTCTTGGTCCTGGGAGA-3' | Wild type Forward | WT: 317bp | 60°C | 28 | 1.5mM | B6;129S6-Sncg<em1(flpo)Tasic>/J | O | |||||||||||||||||||
99 | 5'-TCAGTGTGCCAGGTTGAGAC-3' | Common | Mut: 229bp | |||||||||||||||||||||||||
100 | 5'-GCCTGAAGAACGAGATCAGC-3' | Mutant Forward | ||||||||||||||||||||||||||