FHL2026_Vpec_qPCR_Assay_Aug03_2026_2026filters_eelgrassswab_plate4.xlsx

	A	B	C	D	E	F	G	H	I	J	K	L	M
1	V. pec qPCR assay Aug 12, 2026
2				Each Mix			Goal:	Vpec Taqman qPCR for FHL 2026 Expt
3	Ingredient		per 20ul rxn	98	per 1.5ml tube			Finishing 2026 filter samples and starting
4	Mix		10	1078	539.00			eelgrass swab DNA samples
5	10X Exo IPC Mix		2	216	108.00
6	50 Exo IPC DNA		0.4	43	21.50			Start run
7	VPT4-F (10 um)		0.2	22	11.00			End run
8	VpT4-R (10 um)		0.2	22	11.00			R2	0.954
9	Probe (10 um)		0.2	22	11.00			E	130.00%
10	H20		4.5	485	242.50			Y-int	32.496
11	BSA		0.5	54	27.00			Slope	-2.764
12	Template		2
13	Total		20	1940
14		18 ul of mix
15
16			standards					samples				neg. control
17		1	2	3	4	5	6	7	8	9	10	11	12
18	A	3000000	3000000	3000000	F_T0_03	F_T0_03	F_T0_03	S_ESW_04	S_ESW_04	S_ESW_04	S_EM_03	S_EM_03	S_EM_03
19	B	300000	300000	300000	F_ESW_06	F_ESW_06	F_ESW_06	S_ESW_05	S_ESW_05	S_ESW_05	S_EM_04	S_EM_04	S_EM_04
20	C	30000	30000	30000	F_EM_02	F_EM_02	F_EM_02	S_ESW_06	S_ESW_06	S_ESW_06	S_EMVP_02	S_EMVP_02	S_EMVP_02
21	D	3000	3000	3000	F_EM_06	F_EM_06	F_EM_06	S_EVP_01	S_EVP_01	S_EVP_01	S_EMVP_03	S_EMVP_03	S_EMVP_03
22	E	300	300	300	F_SW_02	F_SW_02	F_SW_02	S_EVP_03	S_EVP_03	S_EVP_03	S_EMVP_04	S_EMVP_04	S_EMVP_04
23	F	30	30	30	F_SW_07	F_SW_07	F_SW_07	S_EVP_04	S_EVP_04	S_EVP_04	S_EMVP_06	S_EMVP_06	S_EMVP_06
24	G	3	3	3	F_BLANK2	F_BLANK2	F_BLANK2	S_EVP_05	S_EVP_05	S_EVP_05	S_Blank_01	S_Blank_01	S_Blank_01
25	H	NTC	NTC	NTC	F_BLANK4B	F_BLANK4B	F_BLANK4B	S_EM_02	S_EM_02	S_EM_02	S_Blank_03	S_Blank_03	S_Blank_03
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