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timeTuesday 07 AugustWednesday 08 AugustThursday 09 AugustFriday 09 August
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9:00 - 9:30Image analysis - theory and practice

Image J Analysis Group BMicroscopy hands on session Group A: DIC, phase contrast, Kohler Illumination and META detector demo Protein dynamics: FRAP, FLIP, photoconversion, photoactivationImageJ - Hands on: Macro programming

- Chapter 2.1 - 2.2
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9:30 -10:00Basics of Basics

- chapter 1.1
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10:00-10:30
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10:30-11:00Introduction to BioimageXD Image J Analysis Group AMicroscopy hands on session Group B: DIC, phase contrast, Kohler Illumination and META detector demo Protein interaction and signalling: FRET and ratiometric imaging
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11:00-11:30Basics of Basics

- chapter 1.1
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11:30-12:00
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12:00-12:30
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12:30-13:00 Introduction and Basic Concepts in Light Microscopy: optical principles, components of microscope, contrast, resolution vs. magnification, optical aberration and correctionsImage analysis Group BMicroscopy hands-on: FRET, FRAP, photoconversion (60 min/group) Group ABioImageXD Tracking
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13:00 - 13:30BioimageXD Hands-on
- dealing with multidimensional data
- visualization
- colocalization with BioimageXD
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13:30 - 14:00Super-resolution microscopy: STORM, PALM, STED, FIONA, SIM, GSD
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14:00 -14:30Image Analysis Group AMicroscopy hands-on: FRET, FRAP, photoconversion (60 min/group) Group B
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14:30-15:00Detectors: digital, CCDs vs scMOS, monochrome vs. colour cameras, dynamic range, bit depth, etc.ImageJ hands-on: Intensity, Filtering (some breaks in between)

- chapter 1.2
- chapter 1.3.1 - 1.3.7
- colocalization with BioimageXDWrap-up: Q&A, discussion, class evaluation
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15:00 - 15:30
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15:30 - 16:00ImageJ Hands-On: background subtraction and segmentation

-chapter 1.4
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16:00 - 16:30Multispectral linear unmixing: tissue imaging
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16:00 - 17:00
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