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TargetCompanyCatalog #AmountHost SpeciesSecondary Information
Channel Recommendations
Product Link
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(per mouse brain hemisphere - multiply by 2 for whole brain)
785 < 488 < 561 < 647
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Primary Antibodies
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Agouti-related protein (AgRP)NeuromicsGT150233 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.neuromics.com/gt15023
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Alpha Smooth Muscle Actin (aSMA)abcamab56943 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/alpha-smooth-muscle-actin-antibody-ab5694.html
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Alpha-synuclein (pS129)abcamab512533 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/alpha-synuclein-phospho-s129-antibody-ep1536y-ab51253.html
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Anti-GFPThermofisherA-111222-5 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.thermofisher.com/antibody/product/GFP-Antibody-Polyclonal/A-11122
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Anti-GFPEncorGPCA-GFP2-5 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://encorbio.com/product/gpca-gfp/
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Anti-RFPRockland600-401-3792-5 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://rockland-inc.com/store/Antibodies-to-GFP-and-Antibodies-to-RFP-600-401-379-O4L_24299.aspx
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Anti-RFPRockland200-101-3792-5 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.rockland.com/categories/primary-antibodies/rfp-antibody-200-101-379/
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Beta-AmyloidCST8243S7.5 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.cellsignal.com/products/primary-antibodies/b-amyloid-d54d2-xp-rabbit-mab/8243?site-search-type=Products
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Beta-AmyloidEncorMCA-AB95 µgMouse IgG2aRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://encorbio.com/product/mca-ab9/
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Beta-AmyloidNovus BiologicalsNBP2-130755 µgMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.novusbio.com/products/beta-amyloid-antibody-moab-2_nbp2-13075
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CalbindinCST463435 µgRabbitRecommend simultaneous Fab staining with 2:1 molar ratio561 / 647
https://www.cellsignal.com/products/primary-antibodies/calbindin-d1i4q-xp-rabbit-mab-bsa-and-azide-free/46343?Ns=product.status.releasedDateIso%7C1&N=4294960086+4294960088&Nrpp=200&fromPage=plp
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CalretininEncorRPCA-Calret5 µLRabbitRecommend sequential staining with 10 µg IgG secondaries per hemisphere561 / 647
https://encorbio.com/product/rpca-calret/
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Cannabinoid Receptor 1CST93815S*2.6 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 647
https://www.cellsignal.com/products/primary-antibodies/cb1-receptor-d5n5c-rabbit-mab/93815
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CD31R&D SystemsAF36285 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.rndsystems.com/products/mouse-rat-cd31-pecam-1-antibody_af3628
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CD45Thermofisher14-0451-822.5 µgRatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/CD45-Antibody-clone-30-F11-Monoclonal/14-0451-82
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cFosabcamab2146721.75 µgRabbitRequires sequential staining with 1.5:1 molar ratio IgG secondaries647
https://www.abcam.com/c-fos-antibody-epr20769-ab214672.html
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ChATMillipore AB144P20 µLGoatRecommend sequential staining with 4 µg IgG secondaries per hemisphere561 / 647
https://www.emdmillipore.com/US/en/product/Anti-Choline-Acetyltransferase-Antibody,MM_NF-AB144P
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CNPEncorRPCA-CNP10 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://encorbio.com/product/rpca-cnp/
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cTip2BioLegend6506042.5 µgRatRecommend IgG sequential staining with 2:1 molar ratio 647
https://www.biolegend.com/en-us/products/go-chip-grade-purified-anti-bcl11b-antibody-14563?GroupID=BLG9392
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D1R (Dopamine receptor 1)NittoboMSFR1010105 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://nittobo-nmd.co.jp/english/product/product_list.php?input_product_name=D1R
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D2R (Dopamine receptor 2)NittoboMSFR1010705 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 647
https://nittobo-nmd.co.jp/english/product/product_list.php?input_product_name=d2r
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Dopamine Beta Hydroxylase (DBH)abcamab2094873 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/dopamine-beta-hydroxylase-antibody-epr20385-ab209487.html
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Gad67ThermofisherMA5-249095 µgMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/GAD67-Antibody-clone-OTI3G9-Monoclonal/MA5-24909
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GFAPBioLegend6447025 µgMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.biolegend.com/en-us/products/purified-anti-gfap-antibody-5753
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GFAPThermofisher13-03005 µgRatRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.thermofisher.com/antibody/product/GFAP-Antibody-clone-2-2B10-Monoclonal/13-0300
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HA-tagCST86124SFSample dependentRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.cellsignal.com/products/primary-antibodies/ha-tag-c29f4-rabbit-mab-bsa-and-azide-free/86124
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Iba-1CST793945 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 488 / 561 / 647
https://www.cellsignal.com/products/primary-antibodies/iba1-aif-1-e4o4w-xp-rabbit-mab-bsa-and-azide-free/79394?N=4294960089+4294960090&fromPage=plp
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Iba-1R&D SystemsNB100-10285 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.novusbio.com/products/aif-1-iba1-antibody_nb100-1028
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Ki-67CST12202S0.5 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.cellsignal.com/products/primary-antibodies/ki-67-d3b5-rabbit-mab-mouse-preferred-ihc-formulated/12202
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Melanin Concentrating Hormone (MCH)abcamab2744152 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/products/primary-antibodies/mch-antibody-epr23778-94-ab274415.html
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MycSanta Cruzsc-40Sample dependentMouse IgG1Recommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.scbt.com/p/c-myc-antibody-9e10
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NeuNCST366623 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.cellsignal.com/products/primary-antibodies/neun-d4g4o-xp-rabbit-mab-bsa-and-azide-free/36662
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NeuNEncorMCA-1B75 µgMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://encorbio.com/product/mca-1b7/
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NeuNEncorRPCA-FOX33 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://encorbio.com/product/rpca-fox3/
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NeuNNovus BiologicalsNBP3-055545 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 647
https://www.novusbio.com/products/rbfox3-neun-antibody_nbp3-05554
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Neuropeptide Y (NPY)CST11976S3 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.cellsignal.com/products/primary-antibodies/neuropeptide-y-d7y5a-xp-rabbit-mab/11976
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NF-HEncorMCA-9B1210 µgMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://encorbio.com/product/mca-9b12/
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nNosabcamab13762 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 647
https://www.abcam.com/nnos-neuronal-antibody-ab1376.html
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Norepinephrine Transporter (NET)MAb TechnologiesNET05-25 µLMouse IgG2bRecommend IgG sequential staining with 2:1 molar ratio 647
https://mabtechnologies.com/categories/product/7-norepinephrine-transporter-mouse-net05-2
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Olfm4CST39141SSample dependentRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.cellsignal.com/products/primary-antibodies/olfm4-d6y5a-xp-rabbit-mab-mouse-specific/39141
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Olig2R&D SystemsAF24183 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.rndsystems.com/products/human-mouse-rat-olig2-antibody_af2418
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Orexin A/BSynaptic Systems389 1043 µLGuinea Pig
Recommend IgG sequential staining with 2:1 molar ratio, assuming primary is 1 mg/mL
561 / 647
https://www.sysy.com/product/389104#list
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Parvalbumin (PV)ThermofisherPA1-9335 µgRabbitRequires simultaneous Fab staining with 2:1 molar ratio561 / 647
https://www.thermofisher.com/antibody/product/Parvalbumin-Antibody-Polyclonal/PA1-933
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PVabcamab328955 µgGoatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/parvalbumin-antibody-ab32895.html
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PGP9.5proteintech14730-1-APSample dependentRabbitRecommend simultanoues Fab staining with 2:1 molar ratio561 / 647
https://www.ptglab.com/products/UCHL1-Antibody-14730-1-AP.htm
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Phospho-CREB (Ser133)Millipore06-5192.5 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.emdmillipore.com/US/en/product/Anti-phospho-CREB-Ser133-Antibody,MM_NF-06-519
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Phospho-Tau (Ser202, Thr205, AT8)ThermofisherMN10205 µgMouse IgG1Recommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/Phospho-Tau-Ser202-Thr205-Antibody-clone-AT8-Monoclonal/MN1020
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S100BThermofisherPA5-781613 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/S100B-Antibody-Polyclonal/PA5-78161
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SFTPCThermofisherPA5-716803 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/SFTPC-Antibody-Polyclonal/PA5-71680
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SNAP-tagNew England BiolabsP9310SSample dependentRabbit
Recommend IgG sequential staining with 2:1 molar ratio assuming primary is 1 mg/mL.
561 / 647
https://www.neb.com/products/p9310-anti-snap-tag-antibody-polyclonal#Product%20Information
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Somatostatin (SST)Santa Cruzsc-477063 µgRatRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.scbt.com/p/somatostatin-antibody-yc7
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Somatostatin (SST)AbclonalA92743 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://abclonal.com/catalog-antibodies/SSTRabbitpAb/A9274
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TPH2ThermofisherPA1-7783 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.thermofisher.com/antibody/product/TPH2-Antibody-Polyclonal/PA1-778
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TREM2abcamab2452273 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/products/primary-antibodies/trem2-antibody-epr23177-106-ab245227.html
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Tyrosine Hydroxylase (TH)BioLegend8180013 µgMouse IgG2aRecommend simultaneous Fab staining with 2:1 molar ratio488 / 561 / 647
https://www.biolegend.com/en-us/products/purified-anti-tyrosine-hydroxylase-antibody-11584
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V5BethylA190-118A3-5 µgChickenRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.fortislife.com/products/primary-antibodies/chicken-anti-v5-tag-antibody/A190-118A
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VimentinCST5471SSample dependentRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.cellsignal.com/products/primary-antibodies/vimentin-d21h3-xp-rabbit-mab/5741
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VIPabcamab2278503 µgRabbitRecommend IgG sequential staining with 2:1 molar ratio 561 / 647
https://www.abcam.com/vip-antibody-c-terminal-ab227850.html
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* = Custom carrier free version recommended for higher concentrations.
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Fluorescent Nuclear Dyes and Other Labels:
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Nuclear dye (488 ex): Yo-Pro-1Biotium4008912 µLN/A
https://biotium.com/product/oxazole-yellow-1mm-in-dmso/?keyword=yo-pro-1&creative=490062241868
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Nuclear dye (561 ex): Propidium IodideThermofisherP356612 µLN/A
https://www.thermofisher.com/order/catalog/product/P3566
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Nuclear dye (642 ex): Sytox Deep RedThermofisherS113815 µLN/A
https://www.thermofisher.com/order/catalog/product/S11381#/S11381
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Vasculature label: Lectin (Dylight-xxx)Vector LabsDL-1178-140 µLN/AAny DyLight conjugate works well
https://vectorlabs.com/dylight-649-labeled-lycopersicon-esculentum-tomato-lectin-lel-tl.html
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Monovalent Fab Fragment Secondary Antibodies:
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Monovalent Fab FragmentsJackson ImmunoresearchCatalog 3See aboveDonkey / GoatCan be used for simultaneous staining. Any in catalog 3 will work.
https://www.jacksonimmuno.com/catalog/3
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488 Excited IgG Secondary Antibodies:
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Donkey anti-RabbitJackson Immunoresearch711-545-152See aboveDonkeyAlexa 488
https://www.jacksonimmuno.com/catalog/products/711-545-152
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Donkey anti-MouseJackson Immunoresearch715-545-151See aboveDonkeyAlexa 488
https://www.jacksonimmuno.com/catalog/products/715-545-151
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Donkey anti-GoatJackson Immunoresearch705-545-147See aboveDonkeyAlexa 488
https://www.jacksonimmuno.com/catalog/products/705-545-147
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Donkey anti-RatJackson Immunoresearch712-545-153See aboveDonkeyAlexa 488
https://www.jacksonimmuno.com/catalog/products/712-545-153
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Donkey anti-ChickenJackson Immunoresearch703-545-155See aboveDonkeyAlexa 488
https://www.jacksonimmuno.com/catalog/products/703-545-155
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Donkey anti-RabbitInvitrogenA32790See aboveDonkeyAlexa 488+
https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32790
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Donkey anti-MouseInvitrogenA32766See aboveDonkeyAlexa 488+
https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32766
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Donkey anti-GoatInvitrogenA32814See aboveDonkeyAlexa 488+
https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32814
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Donkey anti-RatInvitrogenA48269See aboveDonkeyAlexa 488+
https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A48269
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561 Excited IgG Secondary Antibodies:
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Donkey anti-RabbitJackson Immunoresearch711-295-152See aboveDonkeyRRX
https://www.jacksonimmuno.com/catalog/products/711-295-152
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Donkey anti-MouseJackson Immunoresearch715-295-151See aboveDonkeyRRX
https://www.jacksonimmuno.com/catalog/products/715-295-151
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Donkey anti-GoatJackson Immunoresearch705-295-147See aboveDonkeyRRX
https://www.jacksonimmuno.com/catalog/products/705-295-147
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Donkey anti-RatJackson Immunoresearch712-295-153See aboveDonkeyRRX
https://www.jacksonimmuno.com/catalog/products/712-295-153
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Donkey anti-ChickenJackson Immunoresearch703-295-155See aboveDonkeyRRX
https://www.jacksonimmuno.com/catalog/products/703-295-155
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Donkey anti-RabbitInvitrogenA32794See aboveDonkeyAlexa 555+
https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32794
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Donkey anti-MouseInvitrogenA32773See aboveDonkeyAlexa 555+
https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32773
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Donkey anti-GoatInvitrogenA32816See aboveDonkeyAlexa 555+
https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32816
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Donkey anti-RatInvitrogenA48270See aboveDonkeyAlexa 555+
https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A48270
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647 Excited IgG Secondary Antibodies:
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Donkey anti-RabbitLifeCanvas TechnologiesDkxRb-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-MouseLifeCanvas TechnologiesDkxMs-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-RatLifeCanvas TechnologiesDkxRt-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-ChickenLifeCanvas TechnologiesDkxCk-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-GoatLifeCanvas TechnologiesDkxGt-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-Guinea PigLifeCanvas TechnologiesDkxGP-STSee aboveDonkeySeTau-647
https://lifecanvastech.com/shop/setau-647-secondary-antibody/
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Donkey anti-HumanBiotium20076Sample dependentDonkeyCF®633
https://biotium.com/product/donkey-anti-human-igg-hl-highly-cross-adsorbed/