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Kit names CommentsSuplliersTarget RNARNA enrichmentlibrary construction
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Total RNA (lincRNA, snRNA, snoRNA, ...)PolyA (mRNA)Full-length3'Small Non-CodingLong-Non-Coding
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miRNAPiwi-RNALong-Non-Codingcircular RNApoly(A) selectionRNAr depletedKit include library constructionstrand-specificdUTP methodLigation methodOther methodfragmentationmultiplexing capabilities
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TruSeq Stranded mRNAIlluminaXXXXyesyesyesUDIs
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TruSeq RNA Library Prep Kit v2
alos called TruSeq RNA sample Prep Kit v2
https://dnatech.genomecenter.ucdavis.edu/wp-content/uploads/2013/10/datasheet_truseq_sample_prep_kits.pdf
IlluminaXXXXnoyesUDIs
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Illumina Stranded mRNA PrepIlluminaXXXXyesyesyesUDIs
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CORALL mRNA-Seq Library Prep kitRead 1 reflects the RNA transcript sequenceLexogenXXXXyesyesDisplacement Stop Primers (DSP)NOUMIs and UDIs
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KAPA mRNA HyperPrep KitRocheXXXXyesyesyesyes
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Old Kit name (deprecated): KAPA Stranded mRNA-Seq kit
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SMART-Seq v4 PLUS Kit (SSv4 PLUS)for ultra-low RNA inputsTakaraXXXXnoyesyes
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SMART-Seq v4 (SSv4)only mRNA selection + cDNA amplifcationTakaraXXX~ (only cDNA amplification)noNANA
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SMART-Seq® HT PLUS Kit (SS-HT PLUS) TakaraXXXXnoyesyes
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SMART-Seq® HT Kit (SS-HT)only mRNA selection + cDNA amplifcationTakaraXXX~ (only cDNA amplification)noNANA
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Poly(A) RNA Selection KitLexogenXXNONANA
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Poly(A) mRNA Magnetic Isolation ModuleNEBNextXXNONANA
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Oligo d(T)25 Magnetic BeadsNEBNextXXNONANA
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Oligo (dT)25 Cellulose Beads deprecated, on March 31, 2020 "We recommend Oligo d(T)25 Magnetic Beads (NEB #S1419S) as an alternative for purification of mRNA containing polyadenylic (poly A) regions. For NGS applications, the NEBNext Poly(A) mRNA Isolation Module (NEB #E7490) is recommended”"NEBNextXXNONANA
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polyA Spin™ mRNA Isolation KitNEBNextXXNONANA
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TruSeq Stranded Total RNA Human/Mouse/RatRemoves cytoplasmic rRNA, Bead-based rRNA depletionIlluminaXXXXyesyesyesUDIs
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TruSeq Stranded Total RNA GOLD versionremoves both cytoplasmic and mitochondrial rRNAIlluminaXXXXyesyesyesUDIs
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Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Enzymatic rRNA depletion. Ribo-Zero Plus included for depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, bacteria Gram +/- rRNA, human beta globin transcripts
IlluminaXXXXyesyesyesUDIs
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TruSeq Stranded Total RNA with Ribo-Zero Globin
For blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.
IlluminaXXXXyesyesyesUDIs
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KAPA RNA HyperPrep Kit with RiboErase (HMR)Depletion of rRNA by hybridizationRocheXXXXyesyesyesyes
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old Kit name (deprecated): KAPA Stranded RNA-Seq kit with RiboErase (HMP)
X
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KAPA RNA HyperPrep Kit with RiboErase (HMR) GlobinFor blood-derived RNARocheXXXXyesyesyesX
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Ovation® SoLo® RNA-Seq library preparation kit
libraries generated by the Ovation SoLo RNA-Seq System, the forward read corresponds to the sense strand.
Tecan/NuGENXXXXyesyesyes
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SMARTer Stranded Total RNA Sample Prep Kit
only for mammalian, strand information using SMART technology (no ligation, but same resulting reads)
TakaraXXXXyesyesyesX
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SMARTer Stranded RNA-seq kit
for non-mammalian, ONLY lib construction, no RNA selection, strand information using SMART technology (no ligation, but same resulting reads)
TakaraXNOyesyesyesX
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RiboCop rRNA Depletion Kits
3 different kits: RiboCop for Human/Mouse/Rat, RiboCop for Human/Mouse/Rat plus Globin, RiboCop for Bacteria
LexogenXXNONANA
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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) NEBNextXXNONANA
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NEBNext Ultra (II) RNA Library Prep Kit for IlluminaONLY lib constructionNEBNextXXnoyes
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NEBNext® Ultra™ II Directional RNA Library Prep Kit for IlluminaONLY lib constructionNEBNextXXyesyesyes
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KAPA RNA HyperPrep KitONLY lib constructionRocheXXyesyesyes
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old Kit name (deprecated): KAPA Stranded RNA-Seq kitX
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CORALL Total RNA-Seq Library Prep KitONLY lib constructionLexogenXyesyesDisplacement Stop Primers (DSP)NOUMIs
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QuantSeq 3' mRNA-Seq Library Prep Kit FWD/REV for Illumina
Be careful, there is also a kit named QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 (see below ) which is not accepted in bgee because it uses custom primers for target RNA
LexogenXXXXYesStranded with 3'
Combinatoria/uniquel Dual Indexing (UDI) + UMI +
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QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina
Be careful, there is also a kit named QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 (see below ) which is not accepted in bgee because it uses custom primers for target RNA
LexogenXXXXYesStranded with 3'
Sample-barcodes and Unique Molecular Identifiers (UMIs)
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3′ Tag-SeqdnatechXXXYesStranded with 3'UMIs
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MACE-Seq
Improved variant of “3′ single end mRNA Seq” or “Tag-Seq”. In contrast to most other Tag-Seq methods, in MACE-Seq, each molecule is barcoded with a unique sequence (“TrueQuant” -unique molecular identifiers, UMIs) for PCR bias elimination (patented).
GenXproXXXYesStranded with 3'UMIs
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GenXPro/TrueQuant small RNA sequencing kit
Also called 'Gel Free smallRNASeq kit' or 'smallRNA-Seq Kit-Ultra Low Input'
GenXproXXXyes
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Small RNA-Seq Library Prep KitLexogenXXXi7 indexes
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SPLIT RNA Extraction KitTotal RNA or split into small and large RNA fractionslexogenXXXNO
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Ovation® RNA-Seq System V2only cDNA amplificationtecan/NuGENX~ (only cDNA amplification)
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If "PLUS" = also lib contruction
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FWD, generates reads toward the poly(A) tail that correspond to the mRNA sequence during Read 1sequencing. REV, generates reads corresponding to the cDNAsequence during Read 1 sequencing
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