2015 PAGE recipe for OWW
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guests: please use File --> Save a Copy to get your own copy to modify. --Janet
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http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040A.pdf
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2015 SDS-PAGE RECIPES
https://docs.google.com/spreadsheets/d/1TLf1WFbByX-nFWj6_2ygrp5KdDPHyzDyEa3fmysXdwk/edit#gid=1057479987
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You can change the % acrylamide values, the number of gels, and the excess factor. The recipe below will auto-update.
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Janet Matsen
Original recipes:
resolving gel (4%)
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BOTTOM ("resolving") gelBOTTOM ("resolving") gelBOTTOM ("resolving") gel30% acrylamide1.98
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8.5% acrylamide7.5% acrylamide9.5% acrylamideTOP ("stacking") gel
0.5M Tris-HCl, pH 6.8
3.78
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mL/gel4.26gels6gels6gels6gels
1.5M Tris-HCl, pH 8.8
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1.05excess factor1.05excess factor1.05excess factor1.1excess factor10% SDS150
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26.5target total mL26.5target total mL26.5target total mL14.1target total mLH2O9
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TEMED15
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11.9mL H2O12.8mL H2O11.1mL H2O8.5mL H2O10% APS75
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6.6
mL 1.5M Tris-HCl, pH 8.8
6.6mL 1.5M Tris-HCl, pH 8.86.6mL 1.5M Tris-HCl, pH 8.83.6
mL **0.5M Tris-HCl, pH 6.8**
total volume (mL)
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7.5mL acrylamide6.6mL acrylamide8.4mL acrylamide1.9mL acrylamide
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265uL 10% SDS265uL 10% SDS265uL 10% SDS141uL 10% SDS
check total volume:
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132uL 10% APS132uL 10% APS132uL 10% APS71uL 10% APS
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13uL TEMED13uL TEMED13uL TEMED7uL TEMED
This was good for ~7 gels 3/23/2015
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26.5total mL26.5total mL26.5total mL14.1total mL
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NOTE: Their recipe for X% is wrong. Use mine
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150329 recipe generalization
< 2mL recipe for testing ingredients