2015 PAGE recipe for OWW

	A	B	D	E	G	H	J	K	M	N	P	Q
1	guests: please use File --> Save a Copy to get your own copy to modify. --Janet
2	http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040A.pdf
3	2015 SDS-PAGE RECIPES			https://docs.google.com/spreadsheets/d/1TLf1WFbByX-nFWj6_2ygrp5KdDPHyzDyEa3fmysXdwk/edit#gid=1057479987
4	You can change the % acrylamide values, the number of gels, and the excess factor. The recipe below will auto-update.
5	Janet Matsen										Original recipes:	resolving gel (4%)
6			BOTTOM ("resolving") gel		BOTTOM ("resolving") gel		BOTTOM ("resolving") gel				30% acrylamide	1.98
7			8.5	% acrylamide	7.5	% acrylamide	9.5	% acrylamide	TOP ("stacking") gel		0.5M Tris-HCl, pH 6.8	3.78
8	mL/gel	4.2	6	gels	6	gels	6	gels	6	gels	1.5M Tris-HCl, pH 8.8
9			1.05	excess factor	1.05	excess factor	1.05	excess factor	1.1	excess factor	10% SDS	150
10			26.5	target total mL	26.5	target total mL	26.5	target total mL	14.1	target total mL	H2O	9
11											TEMED	15
12			11.9	mL H2O	12.8	mL H2O	11.1	mL H2O	8.5	mL H2O	10% APS	75
13			6.6	mL 1.5M Tris-HCl, pH 8.8	6.6	mL 1.5M Tris-HCl, pH 8.8	6.6	mL 1.5M Tris-HCl, pH 8.8	3.6	mL 0.5M Tris-HCl, pH 6.8	total volume (mL)	15
14			7.5	mL acrylamide	6.6	mL acrylamide	8.4	mL acrylamide	1.9	mL acrylamide
15			265	uL 10% SDS	265	uL 10% SDS	265	uL 10% SDS	141	uL 10% SDS	check total volume:	15
16			132	uL 10% APS	132	uL 10% APS	132	uL 10% APS	71	uL 10% APS
17			13	uL TEMED	13	uL TEMED	13	uL TEMED	7	uL TEMED	This was good for ~7 gels 3/23/2015
18			26.5	total mL	26.5	total mL	26.5	total mL	14.1	total mL
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22	NOTE: Their recipe for X% is wrong. Use mine
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