ABCDEFGHIJKLMNOPQRSTUVWXYZ
1
This is a targeted assay for relative quantitation of histone PTM peptides listed in the panel below.
For sample preparation, please follow the protocol: https://www.jove.com/t/54112/complete-workflow-for-analysis-histone-post-translational
Please stop before this step in the protocol and submit the samples to the core:
6. Chemical Derivatization of Histones Using Propionic Anhydride for Bottom-up Analysis
2
The following PTMs will be monitors (not all may be detected).
3
TKQTAR(H3_3_8)
4
H3_3_8 unmod
5
H3_3_8 K4me1
6
H3_3_8 K4me2
7
H3_3_8 K4me3
8
H3_3_8 K4ac
9
KSTGGKAPR(H3_9_17)
10
H3_9_17 unmod
11
H3_9_17 K9me1
12
H3_9_17 K9me2
13
H3_9_17 K9me3
14
H3_9_17 K9ac
15
H3_9_17 K14ac
16
H3_9_17 K9me1K14ac
17
H3_9_17 K9me2K14ac
18
H3_9_17 K9me3K14ac
19
H3_9_17 K9acK14ac
20
KQLATKAAR(H3_18_26)
21
H3_18_26 unmod
22
H3_18_26 K23me1
23
H3_18_26 K18me1
24
H3_18_26 K18me1K23me1
25
H3_18_26 K18ac
26
H3_18_26 K23ac
27
H3_18_26 K18acK23ac
28
KSAPATGGVKKPHR(H3_27_40)
29
H3_27_40 unmod
30
H3_27_40 K36me1
31
H3_27_40 K27me1
32
H3_27_40 K27me2
33
H3_27_40 K36me2
34
H3_27_40 K27me3
35
H3_27_40 K36me3
36
H3_27_40 K27me2K36me1
37
H3_27_40 K27me1K36me2
38
H3_27_40 K27me1K36me1
39
H3_27_40 K27me3K36me1
40
H3_27_40 K27me1K36me3
41
H3_27_40 K27me2K36me2
42
H3_27_40 K27me3K36me2
43
H3_27_40 K27ac
44
KSAPSTGGVKKPHR(H33_27_40)
45
H33_27_40 unmod
46
H33_27_40 K36me1
47
H33_27_40 K27me1
48
H33_27_40 K27me2
49
H33_27_40 K36me2
50
H33_27_40 K27me3
51
H33_27_40 K36me3
52
H33_27_40 K27me2K36me1
53
H33_27_40 K27me1K36me2
54
H33_27_40 K27me1K36me1
55
H33_27_40 K27me3K36me1
56
H33_27_40 K27me1K36me3
57
H33_27_40 K27me2K36me2
58
H33_27_40 K27me3K36me2
59
H33_27_40 K27ac
60
YRPGTVALR(H3_41_49)
61
H3_41_49 unmod
62
H3_41_49 Y41ph
63
YQKSTELLIR(H3_54_63)
64
H3_54_63 unmod
65
H3_54_63 K56me1
66
H3_54_63 K56me2
67
H3_54_63 K56me3
68
H3_54_63 K56ac
69
EIAQDFKTDLR(H3_73_83)
70
H3_73_83 unmod
71
H3_73_83 K79me1
72
H3_73_83 K79me2
73
H3_73_83 K79me3
74
H3_73_83 K79ac
75
VTIMPKDIQLAR(H3_117_128)
76
H3_117_128 unmod
77
H3_117_128 K122ac
78
GKGGKGLGKGGAKR(H4_4_17)
79
H4_4_17 unmod
80
H4_4_17 K5ac
81
H4_4_17 K8ac
82
H4_4_17 K12ac
83
H4_4_17 K16ac
84
H4_4_17 K5acK8ac
85
H4_4_17 K5acK12ac
86
H4_4_17 K5acK16ac
87
H4_4_17 K8acK12ac
88
H4_4_17 K8acK16ac
89
H4_4_17 K12acK16ac
90
H4_4_17 K5acK8acK12ac
91
H4_4_17 K5acK8acK16ac
92
H4_4_17 K5acK12acK16ac
93
H4_4_17 K8acK12acK16ac
94
H4_4_17 K5acK8acK12acK16ac
95
KVLR(H4_20_23)
96
H4_20_23 unmod
97
H4_20_23 K20me1
98
H4_20_23 K20me2
99
H4_20_23 K20me3
100
H4_20_23 K20ac