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Metadata sheet per Sample
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Enter the information and contact details of the lab, PI and submitter.
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All fields marked by * are mandatory. Enter your details in column E.
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Example values for each field is provided in column D for aid with filling the sheet.
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Make edits only in column E. Do not change the field names
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Enter information and contact details of the lab, PI and submitter in submission information fields.ExampleYour sample details
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Submission InformationPlatform*Sequencing platformLC-MS
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Project ID*Unique ID associated to all the samples of this submissionMS_mRNA
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Submission date*Date when sequencing data were upload10/10/2025
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Submission author*Person doing the submissionJane Doe
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Submission author email*Email from person doing the submissionjane.doe@university.edu
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Laboratory head/PI*PI's last nameSmith
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Contact email*PI's email or other contact emailj.smith@university.edu
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Sample RNASample name*Unique sample name associated to your submissionsmithLab_GM12878_MS_mRNA
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Sample type*RNA biotypemRNA
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Sample description*Description of the sample being processedPoly(A) RNA was selected and fragmented from the source RNA.
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RIN value*RIN value of sample prior to starting library prep9
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Replicate information*Replicate numberRep1
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Digestion related informationDate of digestionDate when the RNA sample was enzymatically digested.10/26/2023
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Amount digestedQuantity of RNA used in the digestion reaction.1 µg
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EnzymeEnzyme(s) applied to digest RNA into nucleosides/nucleotides.Nuclease P1 (NEB, M0660S), Phosphodiesterase I (Mpbio, 0210097880), Alkaline Phosphatase (SigmaAldrich, ALP-2500)
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BufferComposition of the buffer used during digestion.50 mM Tris-HCl, 10 mM MgCl2, pH 7.5
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TemperatureTemperature and duration at which digestion was performed37°C x 30 min
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AdditivesAny additional reagents used to aid digestion or stabilization.Enter any additional reagent used
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Injection related informationMethod referenceCitation or protocol reference for the digestion or analysis method.Mention citation or link to protocol used for method
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Injected volumeVolume of sample injected onto the LC-MS system.20uL
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AmountTotal RNA or nucleoside mass injected for analysis.50pmol
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Chromatography methodLC modelModel name/number of the LC instrument used.Vanquish Horizon UHPLC
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LC manufacturerCompany that produced the LC instrument.Thermo Fisher Scientific
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LC referenceMethod or documentation reference for LC setup.Manufacturer's protocol
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LC serial numberSerial number of the LC system.12345XYZ
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AutosampleAutosampler model or setup used for injection.Vanquish Split Sampler HT
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MPAComposition of mobile phase A.0.1% formic acid in water
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MPBComposition of mobile phase B.0.1% formic acid in acetonitrile
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Flow rateFlow rate of the mobile phase during LC separation.0.4 mL/min
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Column typeDescription of the chromatography column used.Zorbax RRHD C18
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Column lengthLength of the LC column in millimeters.150 mm
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Column diameterInternal diameter of the LC column.2.1 mm
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Particle sizeParticle size of the column packing material.1.8 µm
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GradientSolvent gradient program used for LC separation.5-95% B in 15 min
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MS methodMS modelModel name/number of the mass spectrometer.Orbitrap Exploris 480
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Serial numberCompany that produced the mass spectrometer.Thermo Fisher Scientific
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Source typeSerial number of the mass spectrometer.67890ABC
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Ionization source type (e.g., ESI, MALDI).ESI
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Emitter typeType of emitter used in the ion source.PicoTip
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Emitter diameterInner diameter of the emitter tip.10 µm
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VoltageApplied ionization voltage.3.5 kV
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Source temperatureTemperature of the ion source.300°C
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ReferenceMethod or documentation reference for MS acquisition.See publication ABC
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Acquisition typeGeneral acquisition mode (e.g., DDA, DIA, targeted).DDA (Data-Dependent Acquisition)
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Acquisition methodDetailed acquisition method, scan settings, or program.Full MS scan followed by top 10 HCD fragmentation
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Enter information of the files and md5sum for the files to be uploaded.
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If more files are to be uploaded, add rows below.
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File submissionFile nameUnique file name of the uploaded filesmithLab_GM12878_MS_mRNA_rep1.raw
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File MD5sumMD5sum for the uploaded filee8c5b3a2d1f0e9c8b7a6d5c4b3a2d1f0
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