Gan_fission_yeast_tomo_log_shared
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EMPIAR-10227SpeciesStrainGenotypeTreatmentPhenotypeSampleInstrument(s)KeV(s)Camera(s)Other EMPIARDetails are in these papers
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WT_972h_G0S. pombeMBY99972 h-, WTEMM−N nitrogen starvationG0 quiescencecryosectionsTitan Krios300Falcon II#####
Cai, Tan, 2019
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cdc10_G1S. pombeMBY165(KGY15) cdc10-129, leu1-32, h-temperature upshiftarrest in G1cryosectionsTitan Krios300Falcon II#####
Cai, Tan, 2019
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Graphical summaries
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Datasets indicated in red text
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Feedback form
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Useful commands
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unzip all data in directory "US1363_G1"
lbunzip2 US1363_G1_tilt/*.bz2
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load tilt series numbered 010 to 019 in slicer
3dmod -S US1363_G1_tomo/14oct31a__01*.rec
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load many many tilt series w/o hitting max memory
3dmod -bin 3 14oct31_*.mrc
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Tutorials & tools
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Cryo-EM basics
Caltech cryo-EM course
An online introductory course taught by Grant Jensen
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Cryo-EM advancedEM-University
Includes the above videos and more; taught by Matthijn Vos and Grant Jensen
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SoftwareIMOD
The IMOD package contains both Etomo (reconstruction) and 3dmod (tutorial), along with many other command-line tools
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ReconstructionEtomo text tutorial
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Reconstruction
Etomo video tutorial
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Visualization3dmod tutorial
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Reconstruction & subtomogram analysis
IMOD RML workshop
Additional tutorial data
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Tools & info from our lab
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Our lab
https://www.anaphase.org/
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Useful scripts
https://github.com/anaphaze/ot-tools
Note: these scripts have cryo-EM software dependencies
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