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ItemLinkPriceStorage requirementNotesOrder
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DNA Extraction - Full Stack
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Proteinase Khttps://www.uoftmedstore.com/item_detail.sz?id=1397-20C
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100mM EDTA?
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10mM Tris(pH 7.5)?
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1% SDShttps://www.uoftmedstore.com/item_detail.sz?id=25864Room temp (6 months)Dilute down with distilled water
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https://www.uoftmedstore.com/item_detail.sz?id=25864Room temp (6 months)
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PhenolMedstore doesn't seem to have it?
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Chloroform??
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70% ethanol?? (Probably any)
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Sterile distilled H2O?? (Probably any)
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DNA Extraction - DNEasy Kit
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DNEasy Kithttps://www.uoftmedstore.com/item_detail.sz?id=33008Room temp
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Ethanol 96-100%https://www.uoftmedstore.com/item_detail.sz?id=16Room temp
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DNA Extraction - FroggaBio Kit
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FroggaBio Kithttps://www.uoftmedstore.com/item_detail.sz?id=53327152.96Room tempProtocol at http://www.froggabio.com/dat/FroggaBio/productFiles/575.pdf1
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Absolute ethanolhttps://www.uoftmedstore.com/item_detail.sz?id=1612.6Room tempMay be hard to get1
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PCR
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Mastermixhttps://www.uoftmedstore.com/item_detail.sz?id=593749.96-20CWe can just throw away what we don't use. This is cheap! Contains loafding dye.
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Electrophoresis
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100bp ladderhttps://www.uoftmedstore.com/item_detail.sz?id=1147120.18+25C for 6 months, -20C for 24 months1
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Agarose Gelhttps://www.uoftmedstore.com/item_detail.sz?id=1581845.41Room temp1
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Tris/Borate/EDTA (TBE) Bufferhttps://www.uoftmedstore.com/item_detail.sz?id=152923.19Room temp1
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Distilled Waterhttps://www.uoftmedstore.com/item_detail.sz?id=1578410.09Room tempWill this do? There are multiple grades and taglines available.1
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Loading Dyehttps://www.uoftmedstore.com/item_detail.sz?id=5684127.66Room tempUnncessary if using Frogga mastermix
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Staining solutionhttps://www.uoftmedstore.com/item_detail.sz?id=3083235.24C?
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Ethidium Bromidehttps://www.uoftmedstore.com/item_detail.sz?id=2590515noxious?
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SYBRSafehttps://www.uoftmedstore.com/item_detail.sz?id=1954370Room temp1
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Autoclave / Sterilization
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Pressure Cooker?
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Oven?
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H2O2, gluteraldyhyde, peracetic acid
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Assorted
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Nitrile gloveshttps://www.uoftmedstore.com/item_detail.sz?id=230814.14Room temp1
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Pipettes & tips
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348.57
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Total436.39
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with tax493.12
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