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TASKPerson in Charge (Current)Person in Charge (Previous)ResponsibilitiesLab Member Expectations
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Lab OrganizationTracyTracyCoordinate with Rob, storage allocation, and work flow.Notify in-charge person of any concerns.
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Bench, incubator, and fridge/freezer allocation.
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Assign responsibilities and coodinate lab clean-up.
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Ensure safety rules are being following.
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General Lab DutiesEVERYONEEnsure water carboy is above 25% full at all times. If you notice it is low, FILL IT.
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Place clean glass to be autoclaved in grey bin.
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Clean up after yourself!
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Label all samples for storage and keep fridges/freezers organized.
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Follow all safety guidelines.
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Report any issues/concerns/etc.
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Common Areas Elijah, NicalaRhea, Cristina, SaraSINK: Ensure sink areas are kept cleaned and maintained.If you wash something, come back and place it in the correct spot once dried.
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WESTERN BLOT AREA: Ensure western blot stations are kept cleaned and maintained.Clean up western blot after every use. This includes discarding of any garbage and wiping up any spills.
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UNDERGRAD AREAS: Ensure undergraduate shared spaces are being kept cleaned and nothing is left behind on these benches. Check biohazard vacuum waste.
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Common Inventory & RestockingTracy, RheaTracy, Towfik, GozelTrack inventory of common consumables (media, serum, plasticware, gloves) and update Quartzy as needed. *Check overstock boxes first*If something is running low in stock, notify the in-charge person(s) immediately.
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Re-stock shelves with common supplies as needed.
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Track lab stock of PBS/LB/sterile water and replenish each month.
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Waste ManagementCristina, GozelElijah, AltheaEnsure that proper disposal of biohazardous & cytotoxic waste is being complied with. If not, gently remind offenders of the guidelines. If issue persists, notify Rob. Follow guidelines for proper disposal of biohazardous and cytotoxic waste. If you are unsure, ask in-charge person for a refresher. *Biohazard in yellow, Cytotoxic in red*
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Take full Biohazard waste boxes + containers, cytotoxic waste containers, and broken glass containers to designated area in East Corridor.Place empty chemical bottles in fumehood + notify "Fumehood" in-charge person.
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Replace with empty boxes, bags, bins + containers.Dispose of waste in vacuum flasks before hitting 75% capacity. Add 20mL of bleach and follow liquid biohazard waste guidelines.
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Receiving OrdersMelanie, TracyNicala, Tracy, NaziaCheck receiving area daily for packages. *Don’t forget to check fridge/freezer*.Place consumable items from packages in their designated spots. For temp sensitive items, place in "new arrival" bins in the 4C deli fridge or -20C common supplies freezer.
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Place consumable items from packages in their designated spots. For temp sensitive items, place in designated box/container in the 4C deli fridge or -20C common supplies freezer.Place chemicals in the fumehood and mark this location on the packing slip.
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Place chemicals in the fumehood and let "Chemicals" person know to barcode. Place packing slips in bin above autoclave station.
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Check packing slips daily and move to receieved folder.
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Mark newly received items as 'received' in Quartzy. Enter their final location in the lab.
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If there is a time sensitive package (plasmids, cell line, etc), message in the Lab WhatsApp Group Chat to let the person who ordered know that it is here and needs to be dealt with.
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Chemical Barcoding/DisposalAlaceaJenniferBarcode new chemical arrivals and notify chemical.inventory@torontomu.caPlace empty chemical bottles in fumehood + notify in-charge person to remove barcode.
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Remove & return chemical barcodes once chemical is finished.Read Chemical Barcoding SOP and stay compliant.
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Periodically check chemicals for compliance.
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Microscope RoomsNicala, MariaNicala, MariaEnsure rooms are clean.Clean up after yourself (shut down properly unless someone is using immediately afterwards; clean lens thoroughly, place dust cover on, remove any work materials or garbage).
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Ensure microscopes are functional.Report any issues to in-charge person.
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Ensure no misuse of microscopes.Sign up to use through QReserve and respect the schedule. Do NOT overbook and cancel at the last minute.
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Keep track of authorized users.Follow all training requirements prior to first use.
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Help train new users.
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Ensure users are booking through QReserve and respecting schedule.
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Tissue Culture SouthMaria, Jennifer, TowfikMaria, Alacea, NadaEnsure Botelho Area is cleaned and properly maintained.*See TC South Duties Checklist near sink to know when it is your week to complete the duties*
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Clean tray and change water in the CO2 incubator, twice every week.Clean up after yourself.
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Clean water bath and change water weekly.Re-fill EtOH bottles + common supplies if running low.
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Re-fill EtOH bottles if low.
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Check & clean under the BSC grills every 2nd week of the month. Ant lab will do this on the last week of the month to make it biweekly cleaninings.
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Hot RoomBIOFECTBIOFECTTrain new users.Must complete full training before entering.
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Request waste pick-up.Dispose of all waste appropriately.
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Set up wipe map.Must do wiple tests after your experiments.
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Test for contamination every month or as needed.
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Liquid Nitrogen Cell StorageTracyTracy, MelanieResponsible for accessing, adding, and removing LN2 cell stores.Do NOT access cells in LN2. If in-charge person is not available, then individuals trained for LN2 may access during urgent needs.
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Keep the LN2 inventory up to date.Must inform the in-charge person of this to update the inventories.
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Plasmid StorageTracyTracyTransform bacteria with new plasmids and make a glycerol media stock for -80C.Inform in-charge person of any new plasmids or cells.
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Make mammalian stock for LN2 storage.
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Keep the plasmid inventory up to date.
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Social LeaderMaria, Tracy, NicalaMaria, Tracy, NicalaOrganize events - even if virtual. Gather ideas for get togethers (with or without Rob)Help, contribute, participate.
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Espresso MachineEveryone who uses itEveryone who uses itDo a full clean and descaling of machine on the first of each month.Keep machine clean and in working order.
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CUSTODIAN ROLES
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RoomPerson(s) In Charge
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MicroscopesNicalaJayde
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Hot RoomBIOFECT
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TC RoomSubo
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