SARS-CoV-2_PCR

	A	B	C	D	E	F	G	H	I
1	Assay	Target	Primer	sequence	Flour/quencher	Genome position	Amplicon size	Source	Notes

2	China CDC	nsp10	CCDC-ORF1-Fwd	CCCTGTGGGTTTTACACTTAA		13342	119	http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html
3			CCDC-ORF1-Rev	ACGATTGTGCATCAGCTGA		13442
4			CCDC-ORF1-Probe	CCGTCTGCGGTATGTGGAAAGGTTATGG	FAM/BHQ1	13377
5	China CDC	N	CCDC-N-Fwd	GGGGAACTTCTCCTGCTAGAAT		28881	99
6			CCDC-N-Rev	CAGACATTTTGCTCTCAAGCTG		28958
7			CCDC-N-Probe	TTGCTGCTGCTTGACAGATT	FAM/BHQ1	28934			assay uses TAMRA quencher
8	Hong Kong	nsp14	HKU-ORF1-Fwd	TGGGGYTTTACRGGTAACCT		18778	132	https://academic.oup.com/clinchem/advance-article/doi/10.1093/clinchem/hvaa029/5719336	reverse transcription at 50°C for 5 min, inactivation of reverse transcriptase at 95°C for 20 s, 40 cycles of PCR amplification (Denaturing at 95°C for 5 s; Annealing/Extending at 60°C for 30 s).
9			HKU-ORF1-Rev	AACRCGCTTAACAAAGCACTC		18889
10			HKU-ORF1-Probe	TAGTTGTGATGCWATCATGACTAG	FAM/BHQ1	18849			assay uses IBFQ quencher
11	Hong Kong	N	HKU-N-Fwd	TAATCAGACAAGGAACTGATTA		29145	110
12			HKU-N-Rev	CGAAGGTGTGACTTCCATG		29236
13			HKU-N-Probe	GCAAATTGTGCAATTTGCGG	FAM/BHQ1	29177			assay uses IBFQ quencher
14	Berlin	RdRp	Berlin_RdRp_SARSr-F	GTGARATGGTCATGTGTGGCGG		15431	100	https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045
15			Berlin_RdRp_SARSr-R	CARATGTTAAASACACTATTAGCATA		15505
16			Berlin_RdRp_SARSr-P2	CAGGTGGAACCTCATCAGGAGATGC	FAM/BHQ1	15470			assay uses BBQ quencher
17			Berlin_RdRp_SARSr-P1	CCAGGTGGWACRTCATCMGGTGATGC	FAM/BHQ1
18	Berlin	E	Berlin_E_Sarbeco_F	ACAGGTACGTTAATAGTTAATAGCGT		26269	113
19			Berlin_E_Sarbeco_R	ATATTGCAGCAGTACGCACACA		26360
20			Berlin_E_Sarbeco_P1	ACACTAGCCATCCTTACTGCGCTTCG	FAM/BHQ1	26332			assay uses BBQ quencher
21	US CDC	N	CDC_2019-nCoV_N1-F	GAC CCC AAA ATC AGC GAA AT		28287	72
22			CDC_2019-nCoV_N1-R	TCT GGT TAC TGC CAG TTG AAT CTG		28335
23			CDC_2019-nCoV_N1-P	ACC CCG CAT TAC GTT TGG TGG ACC	FAM/BHQ1	28309
24	US CDC	N	CDC_2019-nCoV_N2-F	TTA CAA ACA TTG GCC GCA AA		29164	67
25			CDC_2019-nCoV_N2-R	GCG CGA CAT TCC GAA GAA		29213
26			CDC_2019-nCoV_N2-P	ACA ATT TGC CCC CAG CGC TTC AG	FAM/BHQ1	29188
27	US CDC	N	CDC_2019-nCoV_N3-F	GGG AGC CTT GAA TAC ACC AAA A		28681	72
28			CDC_2019-nCoV_N3-R	TGT AGC ACG ATT GCA GCA TTG		28732
29			CDC_2019-nCoV_N3-P	AYC ACA TTG GCA CCC GCA ATC CTG	FAM/BHQ1	28704
30	US CDC	Human RNase P	CDC_RP-F	AGA TTT GGA CCT GCG AGC G					Sample control
31			CDC_RP-R	GAG CGG CTG TCT CCA CAA GT
32			CDC_RP-P	TTC TGA CCT GAA GGC TCT GCG CG	FAM/BHQ1
33	YSPH	N	N-Std-T7-Fwd	TAATACGACTCACTATAGGGGAATTGTGCGTGGATGAGGC		28068	1363	unpublished	RNA standard
34			N-Std-Rev	TGTCTCTGCGGTAAGGCTTG		29411
35	YSPH	E	E-Std-T7-Fwd	TAATACGACTCACTATAGGGGCGTGCCTTTGTAAGCACAA		26207	808	unpublished	RNA standard
36			E-Std-Rev	GGCAGGTCCTTGATGTCACA		27016
37	YSPH	nsp14	nsp14-Std-T7-Fwd	TAATACGACTCACTATAGGGTAGTGCTAAACCACCGCCTG		18447	848	unpublished	RNA standard
38			nsp14-Std-Rev	AACTGCCACCATCACAACCA		19275
39	YSPH	RdRp	RdRp-Std-T7-Fwd	TAATACGACTCACTATAGGGAATAGAGCTCGCACCGTAGC		15094	883	unpublished	RNA standard
40			RdRp-Std-Rev	CATCTACAAAACAGCCGGCC		15957
41	YSPH	nsp10	nsp10-Std-T7-Fwd	TAATACGACTCACTATAGGGGTGGGGGACAACCAATCACT		13122	704	unpublished	RNA standard
42			nsp10-Std-Rev	AGACGAGGTCTGCCATTGTG		13806
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