2014_12_19 screen first GeneMorph library with Nash using whole cells
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ABCDEFGH
1
source cells
cell description
library testedGibson tested# of coloniesdate platedtransform detailscell strain
2
1L641P controlNANA12/15 transform of pJ102BL21(DE3)
3
2WT controlNANA12/15 transform of pJ103BL21(DE3)
4
3
negative control
NANA12/15 transform of pJ104BL21(DE3)
5
4GM19GeneMorph19Gibson1212/5/2014BL21(DE3)
6
5GM19GeneMorph19Gibson1412/5/2014BL21(DE3)
7
6GM19GeneMorph19Gibson11112/5/2014BL21(DE3)
8
7GM19GeneMorph19Gibson1512/5/2014BL21(DE3)
9
8GM19GeneMorph19Gibson11512/6/2014BL21(DE3)
10
9GM19GeneMorph19Gibson1912/6/2014
11
10GM19GeneMorph19Gibson1312/6/2014
12
11GM19GeneMorph19Gibson1512/10/2014
restreak sequenced cols 1-5
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12GM19GeneMorph19Gibson1512/12/2014
restreak sequenced cols 11-15
14
13GM19GeneMorph19Gibson1212/12/2014
15
14GM19GeneMorph19Gibson1112/13/2014
16
15GM19GeneMorph19Gibson1612/14/2014
17
16GM19GeneMorph19Gibson1312/15/2014
18
17GM19GeneMorph19Gibson1712/16/2014
19
18GM19GeneMorph19Gibson1712/17/2014
20
19GM19GeneMorph19Gibson13112/18/2014
21
20GM19GeneMorph19Gibson13112/19/2014
22
21GM19GeneMorph19Gibson13112/20/2014
23
22GM20GeneMorph20Gibson220
24
23GM20GeneMorph20Gibson25
25
24GM20GeneMorph20Gibson2>100
26
25GM20GeneMorph20Gibson216
27
26GM20GeneMorph20Gibson2>50
28
27GM20GeneMorph20Gibson25
restreak sequenced cols 6-10
29
30
31
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141216 source colonies
141217 autoinduced wells
141217 export growth wells
timeline
141217 ADH
141218 reagents
141219 reagents
14121? purified ADHs
141119 assay wells
141219 plate1
141218 ADH export
141219 plate2
141219 plate3 12min
141219 plate4 15min
Copy of 141219 plate4
141218 SDS-PAGE