20251019-cgig-cDNA-calcs-polyIC

	A	B	G	H	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y
1	plate_well	sample	vol-RNA-to-actual.uL	vol_0.5.ug-dT_primers_0.5.uL	vol_h2o_to_denature-vol.uL	vol_5x_rxn_buffer.uL	vol_25mM_MgCl2.ul	vol_10mM_dNTPs.uL	vol_RNasin.uL	vol_GoScript-RT.uL	vol_h2o_to_final-vol.uL	vol_final.uL	mfr_final_vol.uL	final_vol_adjustment_ratio	mfr_primer_per_reaction.uL	mfr_5x_buffer_per_reaction.uL	mfr_MgCl2_per_reaction.uL	mfr_dNTPs_per_reaction.uL	mfr_RNAsing_per_reaction.uL	mrf_RT_per_reaction.uL

2	A1	A1C	0.33	1	8.67	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
3	A2	A2C	0.82	1	8.18	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
4	A3	A3C	0.34	1	8.66	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
5	A4	A4C	0.44	1	8.56	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
6	A5	A5C	0.37	1	8.63	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
7	A6	B1C	0.77	1	8.23	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
8	A7	B2C	0.39	1	8.61	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
9	A8	B3C	0.51	1	8.49	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
10	A9	B4C	0.38	1	8.62	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
11	A10	B5C	0.35	1	8.65	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
12	A11	C1C	0.27	1	8.73	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
13	A12	C2C	0.66	1	8.34	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
14	B1	C3C	0.32	1	8.68	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
15	B2	C4C	0.37	1	8.63	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
16	B3	C5C	0.37	1	8.63	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
17	B4	D1C	0.98	1	8.02	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
18	B5	D2C	0.63	1	8.37	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
19	B6	D3C	0.39	1	8.61	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
20	B7	D4C	0.53	1	8.47	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
21	B8	D5C	0.35	1	8.65	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
22	B9	A1M	0.59	1	8.41	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
23	B10	A2M	0.63	1	8.37	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
24	B11	A3M	0.52	1	8.48	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
25	B12	A4M	0.45	1	8.55	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
26	C1	A5M	0.31	1	8.69	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
27	C2	B1M	0.60	1	8.40	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
28	C3	B2M	0.68	1	8.32	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
29	C4	B3M	0.33	1	8.67	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
30	C5	B4M	1.17	1	7.83	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
31	C6	B5M	0.46	1	8.54	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
32	C7	C1M	0.79	1	8.21	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
33	C8	C2M	1.30	1	7.70	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
34	C9	C3M	0.49	1	8.51	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
35	C10	C4M	0.32	1	8.68	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
36	C11	C5M	1.53	1	7.47	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
37	C12	D1M	1.09	1	7.91	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
38	D1	D2M	1.94	1	7.06	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
39	D2	D3M	1.80	1	7.20	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
40	D3	D4M	0.49	1	8.51	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
41	D4	D5M	0.76	1	8.24	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
42	D5	A1T	0.56	1	8.44	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
43	D6	A2T	0.41	1	8.59	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
44	D7	A3T	0.97	1	8.03	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
45	D8	A4T	0.81	1	8.19	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
46	D9	A5T	1.44	1	7.56	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
47	D10	B1T	0.68	1	8.32	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
48	D11	B2T	0.72	1	8.28	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
49	D12	B3T	0.56	1	8.44	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
50	E1	B4T	0.51	1	8.49	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
51	E2	B5T	0.40	1	8.60	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
52	E3	C1T	0.41	1	8.59	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
53	E4	C2T	0.30	1	8.70	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
54	E5	C3T	0.34	1	8.66	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
55	E6	C4T	0.59	1	8.41	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
56	E7	C5T	0.33	1	8.67	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
57	E8	D1T	0.57	1	8.43	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
58	E9	D2T	0.22	1	8.78	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
59	E10	D3T	0.61	1	8.39	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
60	E11	D4T	0.73	1	8.27	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
61	E12	D5T	0.81	1	8.19	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
62	F1	C1PC	0.39	1	8.61	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
63	F2	C2PC	0.46	1	8.54	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
64	F3	C3PC	0.55	1	8.45	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
65	F4	D1PC	0.81	1	8.19	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
66	F5	D2PC	0.58	1	8.42	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
67	F6	D3PC	0.57	1	8.43	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
68	F7	D4PC	0.67	1	8.33	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
69	F8	D5PC	0.48	1	8.52	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
70	F9	D1PM	0.93	1	8.07	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
71	F10	D2PM	0.53	1	8.47	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
72	F11	D3PM	0.32	1	8.68	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
73	F12	D4PM	0.49	1	8.51	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
74	G1	D4PM	0.60	1	8.40	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
75	G2	A1PT	0.27	1	8.73	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
76	G3	A2PT	0.40	1	8.60	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
77	G4	A3PT	0.39	1	8.61	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
78	G5	A4PT	0.51	1	8.49	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
79	G6	A5PT	0.37	1	8.63	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
80	G7	B1PT	0.53	1	8.47	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
81	G8	B2PT	0.28	1	8.72	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
82	G9	B3PT	0.33	1	8.67	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
83	G10	B4PT	0.40	1	8.60	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
84	G11	B5PT	0.58	1	8.42	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
85	G12	C1PT	0.63	1	8.37	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
86	H1	C2PT	0.54	1	8.46	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
87	H2	C3PT	0.36	1	8.64	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
88	H3	C4PT	0.28	1	8.72	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
89	H4	C5PT	0.47	1	8.53	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
90	H5	D1PT	0.30	1	8.70	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
91	H6	D2PT	0.20	1	8.80	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
92	H7	D3PT	0.37	1	8.63	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
93	H8	D4PT	0.41	1	8.59	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
94	H9	D5PT	0.42	1	8.58	8	2.4	2	1	2	14.6	40	20	2	0.5	4	1.2	1	0.5	1
95
96
97			MMLV_master_mix	vol_single rxn	vol_total(uL)
98			5x_rxn_buffer	8	800
99			MgCl2	2.4	240
100			10mM_dNTPs	2	200