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1
TankTreatmentDateSlide NumberGeoduck IDSex# oocytes/follicle (/3)
or
% spermatids/acinus
Size of follicles/aciniConnective Tissue (5=less CT)Stage
(Immature: early active, spent)
(Mature: late active, ripe)
F: % ElongationNotes
2
3Ambient0123111M0-30%Small-Medium1Early active
Most acini have little to no spermatids, few are around 30% spermatids
3
3Ambient0123124M30-50%Medium5Late active
4
3Ambient0123135M0-20%Medium3Early active
Very disorganized tissue- injury? Looks like it disrupts the formation of acini
5
3Ambient01231413F3Small3Early active60
Follicles w/ more oocytes have more on the follicular wall. Oocytes appear well developed- posibly late active but poorly developed?. Some tissue disorganization.
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3Ambient01231517F11Medium-Large5Late active60
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4Ambient012316041M30-40%Small4Late active
Densely packed
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4Ambient012317044M30-40%Medium2Late active
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4Ambient012318045F14Medium5Ripe50
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4Ambient012319050F5Small3Late active40
Lots of CT around groups of follicles but not neccessarily a lot b/w follicles
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4Ambient012320052F12Large5Ripe40
12
1Low01231018M30-60%Medium2Early active
Lare variation in spermatids/acinus
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1Low01232016M<5%Small2Early active
Early in this stage of development
14
1Low01233006F6Small1Early active90
Low oocytes, but futher in development. Many on follicle wall- reabsorbtion?
15
1Low01234003M0-30%Large4Early active
Evenly distributed spermatid %; acini shapes consistent
16
1Low01235013M20-60%Medium2Early active
Highly variable spermatids/acinus
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2Low01236023M0%Small1Very early active
No spermatids present, some spermatogonia activy begining in acini
18
2Low01237028F8Large4Late Active80
Manny oocytes on wall- reabsorbtion?
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2Low01238034F3Small2Early active50-60
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2Low01239037M0-40%Large3Early active
Most acinii have low % spermatids; seems like normal development
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2Low012310040M10-50%Small2Early active
Small, long and thin acini that are somewhat clumped w/ variable % spermatids
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3Ambient022133F???Reabsorbing/Spent/Bad Processing??
Possibly high prescence of lymph and lymphocytes
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3Ambient022188F?Large5Reabsorbing/Spent/Bad Processing??
Lots of damage to oocytes in follicles- fragmented/shriveled
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3Ambient022114 (prev. 014)14 (prev. 014)F12Very Large5Ripe30-40
Variable # oocytes/follicle
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3Ambient022177F6Small3Early active- small follicles
or
Late active- high volume eggs
70
High volume, ripe eggs in some follicles; sample does not capture a lot of follicle
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3Ambient02211515M0-70%Variable5Early active- small acini w/ no spermatids
or
Late active- large acini with lots of spermatids
Variable spermatids % and variable acini size
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3Ambient022166F7Medium-Large5Ripe30
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4Ambient0221042042M0-70%Small-Large5Early active- large, high % spermatids acini
or
Late active- many small, 0% acini
Variable development observed but the sample is not capturing many acini (small cut); egg visible in lumen
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4Ambient0221057057F5Medium4Late Active (few eggs/follicle) or Ripe (high volume eggs)50
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4Ambient0221056056M60-90%Small-Large3Late Active (few eggs/follicle) or Ripe (high volume eggs)
Some small acini with no spermatids present
31
4Ambient0221053053F8Small2Early active70
Few follicles visible in cut
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4Ambient0221060060F9Medium3Early active70
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4Ambient0221059059F32Large5Ripe10-20
Possibly some spent- large follicles with broken connective tissue in between
34
1Low0221012012M90%Large4Ripe
Odd cut- some acini appear small and undeveloped but in other areas they are very large and highly developed (made charateristics based on more developed area)
35
1Low0221009009F14Large5Ripe15-20
Many desnsely packed follicles
36
1Low0221020020M60-90%Large3Late active
37
1Low0221007007M30-60% (non-spent only)Medium-Large3Ripe with some spent
Some acini appear empty near where spermatids were- assuming this means they are spent
38
1Low0221014 (prev. 14)014 (prev. 14)M10-30%Small-Large2Early active
Few acini visible
39
1Low0221002002M70-90%Large5Ripe
40
1Low0221015015F10Medium4Early active70
Many oocytes budding off follicle wall
41
2Low0221025025F6Small-Medium4Late active40
Few follicles visible in cut
42
2Low0221038038M10%Very small1Early active
Stage could be because of odd cut
43
2Low0221032032M10-20%Small2Early active
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2Low0221029029M10-30%Medium-Large3Early active
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2Low0221026026M50-80%Medium3Late Active
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