2014 A=ecl for NADH. What A is expected
Comments
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
$
%
123
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Still loading...
ABCDEFGHIJKLMNOPQRSTUVWXYZAA
1
liquid volume (uL)
200
liquid volume (uL)
75
Try scaling 75uL to this:
2
A = e*c*l
correction factor
1half-area platesA = e*c*l
correction factor
0.7
175µL = 1cm path
3
http://catalog2.corning.com/LifeSciences/en-US/Shopping/ProductDetails.aspx?productid=3679(Lifesciences)&categoryname=HTS%2c+Assay+and+Label-Free+Detection(Lifesciences)%7c96+Well+Assay+Microplates(Lifesciences)%7cClear+96+Well+Assay+Microplates(Lifesciences)%7cHalf+Area+Flat+Bottom+Clear+96+Well+Assay+Microplates(Lifesciences)
4
mM NADHle (1/mM/cm)expected AmM NADHle (1/mM/cm)expected A
5
00.516.44000.267756.4400.4285714286
6
0.10.516.440.328440.10.267756.440.1724310.73899vol (uL)75
7
0.20.516.440.656880.20.267756.440.344862
correction factor
0.8
8
0.30.516.440.985320.30.267756.440.517293
9
0.40.516.441.313760.40.267756.440.689724mM NADHle (1/mM/cm)expected A
10
0.50.516.441.64220.50.267756.440.86215500.34285714296.440
11
0.60.516.441.970640.60.267756.441.0345860.50.34285714296.441.104
12
0.70.516.442.299080.70.267756.441.20701710.34285714296.442.208
13
0.80.516.442.627520.80.267756.441.379448
14
0.90.516.442.955960.90.267756.441.551879
15
10.516.443.284410.267756.441.72431
16
1.10.516.443.612841.10.267756.441.896741Acel
17
1.20.516.443.941281.20.267756.442.0691721.526.440.1164596273
18
1.30.516.444.269721.30.267756.442.241603
19
1.40.516.444.598161.40.267756.442.414034
20
1.50.516.444.92661.50.267756.442.586465
21
1.60.516.445.255041.60.267756.442.758896
22
1.70.516.445.583481.70.267756.442.931327
23
1.80.516.445.911921.80.267756.443.103758
24
1.90.516.446.240361.90.267756.443.276189
25
20.516.446.568820.267756.443.44862
26
27
How much height is expected for a perfect cylinder?
How much height is expected for a perfect cylinder?
28
V150uLV150uL
29
V0.15cm^3V0.15cm^3
30
A of plate6.4cm^2A of plate6.4cm^2
31
expected height
0.0234375cm^2
expected height
0.0234375cm^2
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Loading...
 
 
 
Sheet1
volume of cone