2014 A=ecl for NADH. What A is expected
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ABCDEFGHIJKLMNOPQRSTUVWXYZAA
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liquid volume (uL)
80
liquid volume (uL)
75
Try scaling 75uL to this:
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A = e*c*l
correction factor
1half-area platesA = e*c*l
correction factor
0.7
175µL = 1cm path
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http://catalog2.corning.com/LifeSciences/en-US/Shopping/ProductDetails.aspx?productid=3679(Lifesciences)&categoryname=HTS%2c+Assay+and+Label-Free+Detection(Lifesciences)%7c96+Well+Assay+Microplates(Lifesciences)%7cClear+96+Well+Assay+Microplates(Lifesciences)%7cHalf+Area+Flat+Bottom+Clear+96+Well+Assay+Microplates(Lifesciences)
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mM NADHle (1/mM/cm)expected AmM NADHle (1/mM/cm)expected A
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00.2046.44000.267756.4400.4285714286
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0.10.2046.440.1313760.10.267756.440.1724310.73899vol (uL)75
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0.20.2046.440.2627520.20.267756.440.344862
correction factor
0.8
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0.30.2046.440.3941280.30.267756.440.517293
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0.40.2046.440.5255040.40.267756.440.689724mM NADHle (1/mM/cm)expected A
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0.50.2046.440.656880.50.267756.440.86215500.34285714296.440
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0.60.2046.440.7882560.60.267756.441.0345860.50.34285714296.441.104
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0.70.2046.440.9196320.70.267756.441.20701710.34285714296.442.208
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0.80.2046.441.0510080.80.267756.441.379448
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0.90.2046.441.1823840.90.267756.441.551879
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10.2046.441.3137610.267756.441.72431
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1.10.2046.441.4451361.10.267756.441.896741Acel
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1.20.2046.441.5765121.20.267756.442.0691721.526.440.1164596273
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1.30.2046.441.7078881.30.267756.442.241603
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1.40.2046.441.8392641.40.267756.442.414034
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1.50.2046.441.970641.50.267756.442.586465
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1.60.2046.442.1020161.60.267756.442.758896
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1.70.2046.442.2333921.70.267756.442.931327
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1.80.2046.442.3647681.80.267756.443.103758
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1.90.2046.442.4961441.90.267756.443.276189
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20.2046.442.6275220.267756.443.44862
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How much height is expected for a perfect cylinder?
How much height is expected for a perfect cylinder?
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V150uLV150uL
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V0.15cm^3V0.15cm^3
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A of plate6.4cm^2A of plate6.4cm^2
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expected height
0.0234375cm^2
expected height
0.0234375cm^2
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Sheet1
volume of cone