Cytek Aurora Panel Design Template.xlsx

	A	B	C	D	E	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB	AC	AD
1	4 Laser Cytek Aurora-UV Panel Design Template					23 Color Spillover Spreading Matrix
2	Emission (nm)	UV	Violet	Blue	Red	from\into	BV421	PacB	BV480	BV510	BV570	BV605	BV650	BV711	BV750	BV785	FITC	Alexa 532	PE	PE/Dazzle	PE-Cy5	PerCP	PCP-Cy5.5	PE-Cy7	APC	Alexa 647	Alexa 700	APC/Fire	7-AAD
3	395	BUV395 (2)				BV421	0	6.7	3.8	3.6	1.5	0.9	0.4	0	0	0.2	2.8	0.9	0.6	0	1.6	0.6	0	0	0.2	0	0.1	0	1.3
4	420		BV421 (4)			PacB	1.8	0	4.5	4	2.1	1	0	0	0.2	0.1	4.9	1.7	1.1	0.7	1	0	0	0.2	0	0	0	0.2	0
5	440					BV480	0.6	2.4	0	5.6	4.5	2.5	1.7	0.8	0.3	0.3	1.5	1.4	1.2	1.1	1.4	0.9	0	0	0.3	0	0	0.1	1.2
6	450		PacBlue (1) or eF450			BV510	0.6	1.5	3.6	0	4.7	3.5	2.5	1.5	1.2	1	3.4	1.1	0.7	2.4	3.2	1.4	0	0	1.4	0.5	0.6	0	1.2
7	480		BV480 (4)			BV570	1.4	2	2.6	2	0	4.2	2.3	1.2	1.4	1.3	3.2	1.6	1.2	3.2	4	1.9	1.4	0.5	0	2.7	0.6	0.5	3.1
8	500	BUV496 (2)		GFP		BV605	0.9	1.2	0.7	0.9	1.2	0	3.2	2.3	2.5	2.8	2.3	0.7	1.2	3.3	4.4	2.7	1.3	0.6	1.6	1.8	0.8	0.4	3.6
9	520		BV510 (1)	FITC (2) or Alexa488		BV650	1.2	1.4	1.2	1	0.7	1.9	0	3.2	3.5	2.5	2.3	0.8	1.3	4	6.4	4.2	2.1	0.4	4	4.2	2.1	1.2	4
10	550			SparkBlue 550 (2)		BV711	1	1.4	0.8	1.2	0.5	0.6	1.7	0	6.2	4.9	2.2	0.7	1.2	4.4	7.2	4.9	2.5	0.9	3.4	2.4	4.8	2.7	4.5
11	570	BUV563 (3)	BV570 (2)			BV750	0.8	1.3	0.8	0.7	0.4	0.3	1	2.5	0	7.3	2	0.7	0.7	1.9	2.8	1.7	0.7	1	0.8	1	1.7	2.6	2.4
12	580			PE (5)		BV785	1.2	1.4	1.1	0.6	0	0	0.5	0.9	3.7	0	1.9	0.6	0.4	1.7	2.1	0.9	0.4	1	0.6	0	0.4	2.9	1
13	600		BV605 (3)	PE/Dazzle594 (4)		FITC	0.2	0	0.7	1.3	2.4	1.2	0.6	0.4	0.1	0	0	6.1	4.7	3.2	2.4	0.6	1.2	0.4	0	1.6	0	0.3	2.1
14	660	BUV661 (4)	BV650 (3)		APC (5)	Alexa 532	0	0	0	1.5	2.9	1.2	1	0.4	0	0	6.4	0	6.3	5.1	5	1.7	2.2	0.6	1	3.2	0	0.4	3
15	680			PE-Cy5 (5) & PerCP (1)	Alexa647 (5)	PE	0.5	0.3	0.5	0.8	4.8	2.9	1.8	1.4	0.7	0.5	1.3	1.8	0	7.1	4.6	1.7	2.7	0.7	1.3	3.5	0.4	0.2	4.1
16	690			7-AAD & PerCP-Cy5.5 (2)		PE/Dazzle	0.2	0.1	0.4	0.5	1.7	2.7	1.5	1.7	0.9	0.7	1.4	1	3.8	0	5.3	2.4	3.8	1.1	1.7	4.5	0.5	0.2	5.1
17	700		BV711 (4)		Alexa700 (2)	PE-Cy5	0.3	0.3	0.1	0.2	0.7	0.9	3.6	3.4	2.3	1.7	1	0.6	2.7	11.6	0	7.5	7.3	2.3	6.1	12.1	3.7	2.1	10.6
18	730	BUV737 (4)				PerCP	0.4	0.6	0.6	1.2	0.8	0.8	3.1	3.2	4	2.8	3.9	1.2	2.1	9.2	13.8	0	5.4	1.9	3.9	8.5	2	1.4	9.6
19	750		BV750 (4)		Zombie NIR	PCP-Cy5.5	0	0	0.4	1.3	1	0.6	4	4.9	4.5	3.8	4.5	1.8	2.5	9.4	14.1	8.8	0	3.1	5.6	7.4	4	3.1	10.9
20	780		BV785 (4)	PE-Cy7 (5)	APC-Cy7 or APC/Fire750 (3)	PE-Cy7	0.1	0	0.2	0.3	0.4	0.3	1.7	0.7	1.7	4.3	1.4	0.7	1.5	4.9	6.8	2.9	1.2	0	1.3	2.7	0.9	4	4.6
21	800	BUV805 (4)		PE/Fire810	APC/Fire810 (4)	APC	0.2	0.4	0.3	0.7	0.5	0.8	3.1	1.2	1.4	1.1	2.1	0.7	1.8	6.6	9.7	6.8	4.1	0.5	0	11.4	3.7	2.2	6.6
22						Alexa 647	0.2	0.3	0	0	0.6	0.7	3.2	0.7	0.9	0.7	1.7	0.5	1.5	6.5	9.5	6.9	4	0.5	8.3	0	4	2.4	6.8
23	Best Combinations					Alexa 700	0	0.4	0.1	0.7	0.7	0	1.9	1.3	1.9	1.3	3.5	0.8	0.9	4.4	6.4	4.3	2.5	0.6	3.4	1.9	0	3.4	4.6
24	Good, but hard to find					APC/Fire	0.1	0.1	0.2	0.3	0.2	0.3	0.7	0.5	1.1	1.6	1.7	0.6	0.6	1.5	2.7	1.4	0.6	0.6	1.6	1.4	1.3	0	1.5
25	(5) bright, (1) dim
26	"&" means both colors can be used together. "or" means they are almost identical, avoid together.
27	Avoid assign PE-Cy7, APC-Cy7 tandem dyes to high antigen density markers such as CD45, CD3. Good choice for dim markers like CD25.
28	Fluorochromes with close emission peaks tend to spillover more. Assign them to different cells. Example: BV421 T cells, Pacific Blue B cells.
29	Note:	If you want me to review, please keep the table structure, simply fill in your color/marker. Easy to follow.
30	My 23, 29 color mouse and human panels are included as separate worksheets. I have most of the antibodies, you are welcome to try these panels.
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