| A | B | C | D | E | F | |
|---|---|---|---|---|---|---|
1 | IgG1 | |||||
2 | Monnet et al. | Haddock2.4 Kd | ||||
3 | Mutations | Ratio Definition | Ratio | Kd (nM) | pH ~6.0 | Kd pH ~7.4 |
4 | Wild Type | 1 | 101 | 7.0E-08 | 2.00E-07 | |
5 | M252Y/S254T/T256E (YTE) | 6.70E-08 | 7.60E-08 | |||
6 | H310A | 7.0E-07 | 7.60E-07 | |||
7 | M252Y/S254T/T256E (YTE) H310A | 1.5E-07 | 3.00E-07 | |||
8 | T250Q M428L (Fc-QL) | Fc/Fc-QL | 1 | 1.80E-07 | 2.10E-07 | |
9 | T307A N315D A330V E382V N389T N434Y | Fc/Fc-QL | 28.4 | 5.30E-06 | 5.80E-06 | |
10 | T256N A378V N434Y | Fc/Fc-QL | 27.8 | 26.1 | 4.00E-07 | 1.80E-06 |
11 | N315D N434Y | Fc/Fc-QL | 6.4 | 3.60E-06 | 1.30E-05 | |
12 | N434Y | Fc/Fc-WT | 3.5 | 9.00E-07 | 9.00E-07 | |
13 | N315D | Fc/Fc-WT | 2.1 | 2.90E-08 | 4.20E-07 | |
14 | T256N | Fc/Fc-WT | 1.8 | 4.80E-08 | 1.70E-07 | |
15 | T307A | Fc/Fc-WT | 1.3 | 1.30E-07 | 6.80E-08 | |