20191126_geoduck_hemo_cDNA-calcs
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sample_IDDnased_RNA(ng/uL)vol_to_100ng(uL)vol_dT_primers_1:10_dilution(uL)primer_denature_vol(uL)vol_h2o_to_15uL(uL)vol_5x_rxn_buffer(uL)vol_10mM_dNTPs(uL)vol_MMLV(uL)vol_h2o_to_25uL(uL)vol_final(uL)
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31 H254.0011510.0051.250.013.7425
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39H791.2711512.7351.250.013.7425
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57H205.001159.0051.250.013.7425
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61H47.72.1011511.9051.250.013.7425
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11-08 1H30.93.2411510.7651.250.013.7425
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11-08 2H12.38.131155.8751.250.013.7425
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MMLV_master_mixvol_single rxnnumber of reactionsvol_total(uL)
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5x_rxn_buffer5735
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10mM_dNTPs1.2578.75
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H2O3.65725.55
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MMLV0.170.7
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Add 10uL to each sample.
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