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1 | Provasoli's Enriched Solution (PES) Volume Calculator | |||||
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3 | Instructions & Guidance | |||||
4 | Provasoli's Enriched Solution (PES) is the traditionally used nutrient media for kelp culturing during the hatchery phase. To prepare this solution, you will need a full equipped laboratory (glassware, micropipettes, analytical balances, completely sterile environment, etc.) and have proper chemical storage to house the chemical inventory. The procedure can be complex, so it is best to have someone comfortable in a scientific lab setting prepare the solution. | |||||
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9 | The next tab, "Chemical Ordering List" provides order information from Fisher Scientific, though there are other chemical supplier options such as Sigma Aldrich; if it is decided to go with a different source be sure, to match the chemical formulas and specifications to ensure you are ordering the correct product. | |||||
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14 | It is recommended that you make the volume needed for your hatchery season beforehand. You can calculate how many liters of solution you will need to prepare by entering the size and number of your tanks in the "Hatchery Needs" tab. | |||||
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19 | The final tab of this spreadsheet "PES Mixing" will auto-calculate the quantity of each chemical you will need to make for each of the 4 solutions; there is also guidance around best practices and storage of the solution. | |||||
20 | Resources | |||||
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22 | Redmond 2014 SeaGrant Manual | |||||
23 | This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License | |||||