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Exploring the logical interpretation of test results comparing those using internal controls of a human gene vs a psuedo virus
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What is not included is when the human gene is too high, and therefore the viral genes might be being outcompeted
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External ControlsInternal Controls
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-ve control+ve controlhuman genepseudo virus1+ viral genesResultComment
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--ErrorLoading error, PCR mastermix failure, control contamination (e.g. with PCR inhibitors or RNase), equipment failure (e.g. PCR temperatures wrong), software error
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++ErrorLoading error, PCR mastermix and or control contamination (e.g. with amplified products), equipment failure (e.g. sensors reporting wrong result), software error
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+-ErrorCombination of above error sources
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-++/-++patient +ve
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++-patient -ve
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+/--+patient +veInternal control broken or control out competed
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+--patient -veInternal control broken or control out competed
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-+-ErrorInadequate sampling
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---ErrorInternal control broken, sample contains RNase, sample contains PCR inhibitors
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External ControlsInternal Controls
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-ve control+ve controlhuman genepseudo virus1+ viral genesResultComment
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--Errorsee above
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++Errorsee above
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+-Errorsee above
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-+++patient +ve
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+-patient -ve ?Could be inadequate sampling
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-+patient +veInternal control broken or control out competed
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--ErrorInadequate sampling, Internal control broken, sample contains RNase, sample contains PCR inhibitors
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External ControlsInternal Controls
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-ve control+ve controlhuman genepseudo virus1+ viral genesResultComment
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--Errorsee above
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++Errorsee above
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+-Errorsee above
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-+++patient +ve
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+-patient -ve
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-+patient +veInternal control broken or control out competed
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--ErrorInadequate sampling, Internal control broken, sample contains RNase, sample contains PCR inhibitors
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