20150522_Jake_Oly_cDNA_Calcs
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ABCDE
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Sample ID[DNased RNA] (ng/ul) uL to 250ng RNAuL dT primers (0.5ug/ug RNA) of a 1:10 dilutionH2O to 18.25uL
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DNased 42215 HC181.163.081.2513.92
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DNased 42215 NC179.23.161.2513.84
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DNased 42215 SC178.113.201.2513.80
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DNased 42215 HT1 124.2910.291.256.71
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DNased 42215 NT1 135.187.111.259.89
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DNased 42215 ST1 122.9110.911.256.09
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MASTER MIX
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Single Reaction (uL)
# ReactionsTotals (uL)
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5x buffer5735
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dNTPs (10mM)1.2578.75
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MMLV0.12570.875
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H2O0.3872.66
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Add 6.75uL to each
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Sheet1