Glycerol Stocks

	A	B	C	D	E	F	G	H	I
1		Enzyme	Mutation	Antibiotic	Vector	Cell Line	Note	Date

2	Box K1
3	1	UXS Truncated		AMP	pET15b	BL21		5/12/2008
4	2	UXS Truncated		AMP	pET15b	BL21		5/20/2008
5	3	hUGDH		AMP	pET15b	DE3 PLysS	Replaced with 100 in box K2	6/3/2008
6	4	UXS Truncated	R236H	AMP	pET15b	BL21		6/16/2008
7	5	UXS Truncated	Y231F	AMP	pET15b	BL21		8/11/2008
8	6	UXS Truncated	Y231F	AMP	pET15b	BL21		8/21/2008
9	7	UXS Truncated	R236A	AMP	pET15b	DH5α		8/21/2008
10	8	UXS Truncated	R236A	AMP	pET15b	BL21		8/25/2008
11	9	Human O-GlcNAC Transferase		AMP & CAM	Gateway cloning vector	BL21	includes HA tag	9/24/2008
12	10	Human O-GlcNAC Transferase		AMP & CAM	Gateway cloning vector	BL21	includes HA tag	9/24/2008
13	11	UXS Truncated	G188A	AMP	pET15b	DH5α		1/30/2009
14	12	UXS Truncated	NAD-loop deletion	AMP	pET15b	DH5α		1/30/2009
15	13	UXS Truncated	Y168H	AMP	pET15b	DH5α		1/30/2009
16	14	hUGDH	9 lysines mutant	AMP	pET15b	BL21		1/30/2009
17	15	hUGDH	9 lysines mutant	AMP	pET15b	C41 cells		1/30/2009
18	16	hUGDH	9 lysines mutant	AMP	pET15b	C41 cells		1/30/2009
19	17	hUGDH	9 lysines mutant	AMP	pET15b	GenX Cells		1/30/2009
20	18	hUGDH	9 lysines mutant	AMP	pET15b	BL21 pLysS		1/30/2009
21	19	hUGDH	9 lysines mutant	AMP	pET15b			1/30/2009
22	20	UXS Truncated	NAD-loop deletion	AMP	pET15b	BL21 GenX Cells		1/30/2009
23	21	UXS Truncated	NAD-loop deletion	AMP	pET15b	BL21 pLysS		1/30/2009
24	22	UXS Truncated	NAD-loop deletion	AMP	pET15b	Rosetta2		1/30/2009
25	23	UXS Truncated	NAD-loop deletion	AMP	pET15b	BL21		1/30/2009
26	24	UXS Truncated	G188A	AMP	pET15b	BL21		1/30/2009
27	25	UXS Truncated	G188A	AMP	pET15b	BL21 pLysS		1/30/2009
28	26	UXS Truncated	G188A	AMP	pET15b	Rosetta2		1/30/2009
29	27	UXS Truncated	G188A	AMP	pET15b	C41 cells		1/30/2009
30	28	UXS Truncated	Y168H	AMP	pET15b	BL21		1/30/2009
31	29	UXS Truncated	Y168H	AMP	pET15b	BL21 GenX Cells		1/30/2009
32	30	UXS Truncated	Y168H	AMP	pET15b	BL21 pLysS		1/30/2009
33	31	UXS Truncated	Y168H	AMP	pET15b	Rosetta2		1/30/2009
34	32	UXS Truncated	Y168H	AMP	pET15b	C41 cells		1/30/2009
35	33	TEV		AMP & CAM	pTh24	Rosetta pLysS
36	34	JBP2 Full-Length
37	35	JBP2 Truncated (1-540)
38	36	JBP1 LT
39	37	Similar to ArnA (Maors Lab?)					Makes a lot of ketose, and little xylose
40	38	hUGDH	Δ132	AMP	pET15b	Rosetta pLysS
41	39	cUGDH		KAN & CAM	pET24D	Rosetta pLysS	From Doering lab
42	40	pgUGDH		AMP	pET15b	Rosetta pLysS		10/26/2009
43	41	pgUGDH		AMP	pET15b	Rosetta pLysS	Dilute	11/4/2009
44	42	pgUGDH		AMP	pET15b	Rosetta pLysS	Dilute	11/4/2009
45	43	UXS Truncated	Y231F and NAD-loop deletion	AMP	pET15b	DH5α		11/6/2009
46	44	hUGDH	C276A	AMP	pET15b	DH5α		11/6/2009
47	45	hUGDH	D280N	AMP	pET15b	DH5α
48	46	hUGDH	K279A	AMP	pET15b	DH5α
49	47	C. Neoformans UXS			pET28A		+HIS, replate before use
50	48	C. Neoformans UXS			pET24D		-HIS, replate before use
51	49	C. Neoformans UGDH			pET24D	DH5α	replate before use
52	50	C. Neoformans UXS			pProEXTC	DH5α	replate before use
53	51	C. Neoformans UGDH			pProcEXHT	DH5α	replate before use
54	52	ArnA (E. coli)		KAN	pMS159	Rosetta pLysS	decarboxylase domain (from M. Sousa lab)	2/9/2010
55	53	UXS Truncated	NADH loop modification (VΔFFT)G(ΔR)	AMP	pET15b	DH5α		5/25/2010
56	54	hUGDH	D280N	AMP	pET15b	Rosetta pLysS		5/28/2010
57	55	hUGDH	C276A	AMP	pET15b	Rosetta pLysS		5/28/2010
58	56	UXS Truncated	NADH loop modification (VΔFFT)G(ΔR)	AMP	pET15b	Rosetta pLysS		5/28/2010
59	57	UXS Truncated	NADH loop modification (VΔFFT)G(ΔR)T176A	AMP	pET15b	Rosetta pLysS		5/28/2010
60	58	UXS Truncated	NADH loop modification (VΔFFT)G(ΔR)T176A	AMP	pET15b	DH5α		6/4/2010
61	59	hUGDH (full-length)	C276A	AMP	pET15b	BL21-T1R
62	60	hUGDH (full-length)	A225L	AMP	pET15b	BL21-T1R
63	61	hUGDH (full-length)	D280N	AMP	pET15b	BL21-T1R
64	62	hUGDH (full-length)	K279A	AMP	pET15b	BL21-T1R
65	63	hUGDH (full-length)	K94E	AMP	pET15b	BL21-T1R
66	64	ArnA	F125A	KAN	pET-28	DH5α
67	65	ArnA	F125A	KAN	pET-28	BL21-T1R
68	66	ArnA		KAN		BL21-T1R
69	67	UXS Truncated	NAD-loop deletion (ΔFFT)	AMP	pET15b	BL21-T1R		6/8/2011
70	68	ArnA		KAN		BL21-T1R	very slow growth
71	69	GDH		KAN		BL21-T1R
72	70	CgIMPDH		KAN		BL21-T1R
73	71	hUGDH Truncated (1-465)	A225L	AMP		BL21-T1R
74	72	hGHPR		AMP	pSGX3	BL21-T1R
75	73	KPR		KAN	pSGX3	BL21-T1R
76
77	Box K2
78	74	KPR	I244A	KAN	pSGX3	BL21-T1R
79	75	KPR	A181L	KAN	pSGX3	BL21-T1R	(S. aureus)
80	76	UXS	P148A & Y150A	AMP	pET15b	BL21-T1R
81	77	UXS				BL21-T1R	(wild-type)
82	78	UXS	P148A & Y150A	AMP	pET15b	BL21-T1R
83	79	hUGDH Truncated	E165Q	AMP	pET15b	BL21-T1R
84	80	PUC18		AMP		pLysS		9/25/2011
85	81	hUGDH (wild-type) + ArnA		AMP & KAN		Rosetta pLysS		4/19/2010
86	82	hUGDH Truncated (1-465)		AMP	pET15b	BL21-T1R		7/18/2012
87	83	KPR		KAN	pSGX3	BL21-T1R		7/18/2012
88	84	Glutathione Reductase		AMP	pET14b	BL21-T1R	B. Henselae
89	85	hUGDH Truncated (1-465)	A82L	AMP	pET15b	BL21-T1R
90	86	A415LGR		AMP	pET14b		B. Henselae
91	87	L417FGR		AMP	pET14b		B. Henselae
92	89	KPR	A201L	KAN		BL21
93	90	hUGDH Truncated (1-465)	R141C	AMP		BL21
94	91	hUGDH Truncated (1-465)	F323-N324-T32S-V236 T327 to D323 D325 V326 V327	AMP	pET15b	BL21	M11 Dimer (Truncated)
95	92	hUGDH	R141C	AMP	pET15b	DH5α			WRONG?
96	93	hUGDH	T253W & E250H	AMP	pET15b	BL21		8/5/2013
97	94	hUGDH	T253W	AMP	pET15b	BL21		1/23/2014
98	95	hUGDH	Y473E	AMP	pET15b	BL21		2/27/2014
99	96	ID-Peptide hUGDH		KAN		BL21	ID-tail peptide construct 1	2/27/2014
100	97	ID-Peptide hUGDH		AMP	pET14b		ID-tail peptide construct 2	3/12/2014