PEI transfection calculator for AAV packaging
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ABCDEFGHIJKLMNOPQRSTUV
1
molar ratio3:5:2
2
15 cm plates OR CF5 stacks?
15 cm
3
number of plates/stacks
1
4
5
6
7
Viral Prep NamePlasmids
Size (bp)
Concentration (ug/ul)
ug per plate or stack
total ug of plasmid
total ul of plasmid
8
transfer plasmid
58740.655.455.458.39
9
pHelper116351.6118.0118.0111.16
10
RepCap73281.184.544.543.84
11
transfer plasmid
71224.666.356.351.36
12
pHelper116353.7017.2917.294.67
13
RepCap73280.984.364.364.45
14
transfer plasmid
50001.0014.2414.2414.24
15
pHelper25001.0011.8611.8611.86
16
RepCap10001.001.901.901.90
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