20160316 - WS p18RK7 plasmid dilution table
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ABCDEFGH
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mass of one plasmid molecule (g/copy)plasmid size (bp)DNA mass (g/bp)
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5.95E-1854321.10E-21
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Copy #mass of one plasmid molecule (g/copy)Mass of Plasmid DNA (g)Mass of Plasmid (ng)
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30121502.211.79E-100.17932752
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3,000,0001.79E-110.017860416
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300,0005.95E-181.79E-120.0017860416
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30,0001.79E-130.00017860416
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3,000 1.79E-140.000017860416
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3001.79E-150.0000017860416
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301.79E-160.00000017860416
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31.79E-170.000000017860416
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105.95E-170.00000005953472
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84.76E-170.000000047627776
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63.57E-170.000000035720832
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42.38E-170.000000023813888
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21.19E-170.000000011906944
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15.95E-180.000000005953472
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0.52.98E-180.000000002976736
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0.251.49E-180.000000001488368
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0.21.19E-180.0000000011906944
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0.158.93E-190.0000000008930208
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0.15.95E-190.0000000005953472
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0.052.98E-190.0000000002976736
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0.015.95E-200
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Copy #Mass of Plasmid DNA (ng)Microliters of Solution per reactionFinal Concentration of plasmid DNA (ng/ul)
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300000000.179327520.08966376
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3,000,0000.0179327520.008966376
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300,0000.001793275220.0008966376
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30,0000.000179327520.00008966376
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3,0000.0000179327520.000008966376
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3000.00000179327520.0000008966376
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300.000000179327520.00000008966376
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30.0000000179327520.000000008966376
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100.000000059775840.00000002988792
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80.0000000478206720.000000023910336
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60.0000000358655040.000000017932752
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40.0000000239103360.000000011955168
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20.0000000119551680.000000005977584
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10.0000000059775840.000000002988792
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0.50.0000000029887920.000000001494396
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0.250.0000000014943960.000000000747198
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0.20.0000000011955170.0000000005977585
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0.150.0000000008966380.000000000448319
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0.10.0000000005977580.000000000298879
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0.050.0000000002988790.0000000001494395
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0.0100
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Dilution #Source of plasmid DNA for dilutionInitial Conc. (ng/ul) CiVol. of plasmid DNA (ul) ViVolume of diluent (ul)Final Volume (ul) VfFinal Conc. (ng/ul) CfResulting Copy # per 2 ul
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1Tstock293.09996.911000.000.0896637630000000
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2TDilution 10.08966376100.00900.001000.000.0089663763000000
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3TDilution 20.008966376100.00900.001000.000.0008966376300000
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4TDilution 30.0008966376100.00900.001000.000.0000896637630000
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5TDilution 40.00008966376100.00900.001000.000.0000089663763000
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6TDilution 50.000008966376100.00900.001000.000.0000008966376300
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7TDilution 60.0000008966376100.00900.001000.000.0000000896637630
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8TDilution 70.00000008966376100.00900.001000.000.0000000089663763
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7296.91
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p18RK7