Calculating quantities of cleaned, indexed PCR to pool

AG notes: Changed calculations to follow formula below

Automatically add in 113 bp in calculation.

Please follow the instructions below. Only cells highlighted in blue need to be modified.

1. Modify the following values as needed:

Final volume desired

Desired concentration of pool

Number of samples

Length of indexing adapter

Cells are protected

3. Input the average size of the library preparation in base pairs in Column C (do not include length of index adapters)

4. Hit the tab key 1 time and the following will be displayed automatically:

Calculated molecular weight of the library based on average fragment size.

nM conversion of library concentration.

Amount of samples that must be added to pool.

Total expected volume from pooled samples.

Volume of water that must be added to pooled samples to reach the desired final volume.

5. Pay attention to the cells highlighted in green- they provide the quantities of samples and water you need to reach your desired volume