ABCDEFGHIJKLMNOPQRSTUVWXYZ
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in general...
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# of reactions
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prepare as if there were
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per reactiontotal to make of mastermix
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reagent
quantity (units)
reagent
quantity (units)
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Generic Thing AGeneric Thing A
quantity per reaction of Generic Thing A * "prepare as if there were" number
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Generic Thing BGeneric Thing B
quantity per reaction of Generic Thing B * "prepare as if there were" number
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Generic Thing CGeneric Thing C
quantity per reaction of Generic Thing B * "prepare as if there were" number
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Generic Thing DGeneric Thing D
quantity per reaction of Generic Thing B * "prepare as if there were" number
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unique thing
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final volume
sum of all above columns
volume mastermix to make
sum of all above columns
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amount of master mix per sample
sum of all generic
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an example (for an RNA labeling reaction)
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# of reactions8
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prepare as if there were9.5
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per reactiontotal to make of mastermix
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reagent
quantity (μL)
reagent
quantity (μL)
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10X PNK buffer510X PNK buffer47.5
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water37water351.5
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hot ATP5hot ATP47.5
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PNK1PNK9.5
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RNA (at 50 μM)2
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final volume50volume mastermix to make456
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amount of master mix per sample48
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