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Morris Water Maze
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File Folders will Optimally Contain: Study Metadata File, Processed Data Outcomes File, Image of arena and setup, Raw Training Session Video, Raw Testing Session Video?Measurement: Behavior
Ontology: http://purl.obolibrary.org/obo/NCIT_C16326
Source: NCIT		Name of person who drafted, and date it was drafted.Instructions:

Please provide your name at the top of your column in case we have any questions or requests for clarification.

For the below parameters (rows), please provide any feedback or suggestions. This could be suggested edits to the parameter, suggested removal (if you believe it's unnecessary), etc.

The last section is a freeform feedback section, where you can provide any general feedback or recommendations that we should take into consideration.

General Info on assay metadata configurations can be found here.
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Supporting Literature:Technology: Morris Water Maze
Ontology: http://purl.bioontology.org/ontology/MESH/D000084443
Source: MESH
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SME Name:SME Name:SME Name:
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Sample Metadata which is in a separate table:e.g. n, age, sex, organism, strain, animal source, cell line, cell type, genotype, habitat, feeding schedule, diet, 'factor value' (SF, GC, Viv; or Independent Variables), light cycle, water, radiation exposure metadata (radiation distance, absorbed radiation doe, absorbed radiaition dose rate, LET, ionizing source, radiation device, particle charge, HZE, radiation energy), any preservation, fixative, euthanasia metadata
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ParameterExample ValueColumn TypeColumn Type Options:ParametersFeedbackFeedbackFeedback
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Experimental DesignProtocolProtocolExperimental Design
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List Other Behavior Assays Being Performed On Subjectse.g. EPM, RAWMFree TextFree TextList Other Behavior Assays Being Performed On Subjects
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Describe Sequence and Timing of All Assayse.g. EPM on first day, Operant on days 3-10, RAWM on day 12Free TextListDescribe Sequence and Timing of All Assays
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Interval Between Assayse.g. 24 hoursUnitUnitInterval Between Assays
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Interval Since Previous Assaye.g. 24 hoursUnitOntologyInterval Since Previous Assay
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Describe Subject Habituation by Handling Prior to Behavior Assayse.g. handled daily in 5 minute sessionsFree TextIntegerDescribe Subject Habituation by Handling Prior to Behavior Assays
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Total Number of Subject Testing Dayse.g. 1-2UnitTotal Number of Subject Testing Days
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Total Number of Experimenters Handling Animals Throughout Assaye.g. 3Free TextTotal Number of Experimenters Handling Animals Throughout Assay
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Were Animals Handled By The Same Experimenters Throughout Entire Assaye.g. Yes/NoFree TextWere Animals Handled By The Same Experimenters Throughout Entire Assay
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Sex of Experimenters Handling Animalse.g. Male, Female, Male and FemaleFree TextSex of Experimenters Handling Animals
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Acclimation Time in Testing Roome.g. 20 minutesUnitAcclimation Time in Testing Room
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Phase of Light Cycle Assay Performed e.g. LightFree TextPhase of Light Cycle Assay Performed
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Clock Time of Assay Starte.g. 22:41Free TextClock Time of Assay Start
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Assay Start Time in Relative to Light Transition e.g. 180 minutes into the dark cycleFree TextAssay Start Time in Relative to Light Transition
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Duration Subject Has Been Maintained on Current Light Cyclee.g. 1 weekUnitDuration Subject Has Been Maintained on Current Light Cycle
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Did Subject Light Cycle Remain Consistent Throughout Entire Operant Conditioning Assaye.g. yes/noFree TextDid Subject Light Cycle Remain Consistent Throughout Entire Operant Conditioning Assay
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Describe Any Changes in Subject Light Cyclee.g. reversed one week priorFree TextDescribe Any Changes in Subject Light Cycle
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Describe Cases for Censorship or Subject Exclusion from Trialse.g. animals exhibited signs of stressFree TextDescribe Cases for Censorship or Subject Exclusion from Trials
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Additional parameters to add?
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Testing EnvironmentProtocolTesting Environment
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Temperature of Testing Room e.g. 15ᴼCUnitTemperature of Testing Room
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Relative Humidity of Testing Room e.g. 75.0%UnitRelative Humidity of Testing Room
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Characterize Sound and Noise Environment of Testing Roome.g. white noise generatorFree TextCharacterize Sound and Noise Environment of Testing Room
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Noise Intensity in Testing Roome.g. 180 dbUnitNoise Intensity in Testing Room
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Describe Any External Cues Present During Testinge.g. multiple technicians, other experiments being performedFree TextDescribe Any External Cues Present During Testing
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Cleaning Solutions Usede.g. 70% EtOHFree TextCleaning Solutions Used
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Frequency of Arena Cleaninge.g. In between each subjectFree TextFrequency of Arena Cleaning
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Arena DescriptionProtocolArena Description
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Pool Vendor Name Model and Catalog Numbere.g. Custom-built, 120 cm diameterFree TextPool Vendor Name Model and Catalog Number
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Pool Shapee.g. CircularFree TextPool Shape
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Pool Dimensions (Diameter × Depth)e.g. 120 cm × 50 cmUnitPool Dimensions (Diameter × Depth)
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Water Depthe.g. 30 cmUnitWater Depth
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Water Temperaturee.g. 22°CUnitWater Temperature
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Water Coloration Methode.g. Nontoxic white paintFree TextWater Coloration Method
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Describe Any Visual Cues Inside Poole.g. Striped patterns on tank wallFree TextDescribe Any Visual Cues Inside Pool
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Describe Any Visual Cues Outside Poole.g. Posters, shelving unitFree TextDescribe Any Visual Cues Outside Pool
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Placement of Video Camerae.g. OverheadFree TextPlacement of Video Camera
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Define Light Source Inside Roome.g. LED overhead lightingUnitDefine Light Source Inside Room
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Color Spectrum of Room Light Sourcee.g. White (6000K)UnitColor Spectrum of Room Light Source
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Luminosity of Room Light Sourcee.g. 20 luxUnitLuminosity of Room Light Source
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Platform Materiale.g. Transparent acrylicFree TextPlatform Material
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Platform Diametere.g. 10 cmUnitPlatform Diameter
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Platform Height From Pool Floore.g. 28 cm (2 cm below water surface)UnitPlatform Height From Pool Floor
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Platform Position for Traininge.g. Northwest quadrantFree TextPlatform Position for Training
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Platform Position for Probe Triale.g. Removed or moved to opposite quadrantFree TextPlatform Position for Probe Trial
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Assay Characteristics - Acquisition and TestingProtocolAssay Characteristics - Acquisition and Testing
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Subject Loading Procedure/Location/Directione.g. Released from edge of pool facing the wallFree TextSubject Loading Procedure, Location, and Direction
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Number of Training Dayse.g. 4Free TextNumber of Training Days
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Number of Trials per Daye.g. 4Free TextNumber of Trials per Day
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Interval Between Trialse.g. 10 minutesUnitInterval Between Trials
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Maximum Trial Duratione.g. 60 secondsUnitMaximum Trial Duration
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Escape Criterion (If Subject Reaches Platform)e.g. 3 seconds on platformUnitEscape Criterion (If Subject Reaches Platform)
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Describe Cases for Technician Interventione.g. If subject floats for >10 seconds, it is guided to platformFree TextDescribe Cases for Technician Intervention
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Describe Procedure If Subject Fails to Find Platform
e.g. Gently guided to platform and allowed to remain for 10 secondsFree TextDescribe Procedure If Subject Fails to Find Platform
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Probe Trial Conducted?e.g. YesFree TextProbe Trial Conducted?
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Time Since Last Training Triale.g. 24 hoursUnitTime Since Last Training Trial
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Platform Removed for Probe Trial?e.g. YesFree TextPlatform Removed for Probe Trial?
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Probe Trial Duratione.g. 60 secondsUnitProbe Trial Duration
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Number of Probe Trialse.g. 1Free TextNumber of Probe Trials
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Subject Starting Position in Probe Triale.g. Opposite quadrant of prior platform locationFree TextSubject Starting Position in Probe Trial
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Describe Any Balancing Measurese.g. Randomized starting locationsFree TextDescribe Any Balancing Measures
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Assay Characteristics - ReversalProtocolAssay Characteristics - Reversal
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Platform Relocated?e.g. YesFree TextPlatform Relocated?
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New Platform Quadrante.g. SoutheastFree TextNew Platform Quadrant
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Reversal Training Duratione.g. 3 daysUnitReversal Training Duration
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Reversal Probe Trial Conducted?e.g. YesFree TextReversal Probe Trial Conducted?
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Analysis CharacteristicsProtocolAnalysis Characteristics
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Describe Interactions Excluded from Measuremente.g. climbing on top of the cage was excluded from total interaction time with the social mouseFree TextDescribe Interactions Excluded from Measurement
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Describe Criteria for Exclusion from Analysise.g. outlier in responses, subject did not complete all phasesFree TextDescribe Criteria for Exclusion from Analysis
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Number of Observers Used for Scoring Subjecte.g. 2Free TextNumber of Observers Used for Scoring Subject
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Tracking and Analysis Systeme.g. Ethovision XT 12.0Free TextTracking and Analysis System
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Quantification Method e.g. Human, semi-automated, (time in interaction zone was quantified by Ethovision and independently quantified by a human and averaged; If there was >5% disagreement, a second human was added, and the two closest scores were averaged)Free TextQuantification Method
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Body Marker Trackede.g. nose point, center pointFree TextBody Marker Tracked
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Subset of Session Used for Deriving Outcome Measurese.g. first 3 minutes, first 5 minutesFree TextSubset of Session Used for Deriving Outcome Measures
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Outcome/Dependent Variable Measures
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Data/MeasureUnit(s)Notes
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Basically: Tabular File Column Names. Include what the measure are, units, etc.
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