20151106 - Dilution table RLOv_DNA_helicase_qPCR_Standard_Curve
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ABCDEFGH
1
Vector pCR2.1
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3
mass of one plasmid molecule (g)plasmid size (bp)mass (g/bp)Vector size (bp)insert size (bp)
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4.55E-1841551.10E-213929226
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6
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Copy #mass of one plasmid molecule (g)Mass of Plasmid DNA (g)Mass of Plasmid (ng)
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300000000.00000000013661640.1366164
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3,000,00000.01366164
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300,0004.55E-1800.001366164
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30,00000.0001366164
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3,000 00.00001366164
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30000.000001366164
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3000.0000001366164
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1000.0000000455388
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300.00000001366164
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100.00000000455388
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Copy #Mass of Plasmid DNA (ng)Microliters of Solution per reactionFinal Concentration of plasmid DNA (ng/ul)
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300000000.13661640.0683082
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3,000,0000.013661640.00683082
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300,0000.00136616420.000683082
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30,0000.00013661640.0000683082
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3,0000.000013661640.00000683082
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3000.0000013661640.000000683082
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300.0000001366164 0.0000000683082
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100.00000004553880.0000000227694
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30.000000013661640.00000000683082
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10.000000004553880.00000000227694
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Dilution #Source of plasmid DNA for dilutionInitial Conc. (ng/ul) CiVol. of plasmid DNA (ul) ViVolume of diluent (ul)Final Volume (ul) VfFinal Conc. (ng/ul) CfResulting Copy # per 2 ul
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1Astock15.497952694.41995.591000.000.068308230000000
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2ADilution 10.0683082100.00900.001000.000.006830823000000
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3ADilution 20.00683082100.00900.001000.000.000683082300000
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4ADilution 30.000683082100.00900.001000.000.000068308230000
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5ADilution 40.0000683082100.00900.001000.000.000006830823000
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6ADilution 50.00000683082100.00900.001000.000.000000683082300
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7ADilution 60.000000683082100.00900.001000.000.000000068308230
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8ADilution 70.0000000683082333.33666.671000.000.000000022769410
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9ADilution 80.0000000227694300.00700.001000.000.000000006830823
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10ADilution 90.00000000683082333.33666.671000.000.000000002276941
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8428.93
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