2013_12_11 NADH comparison on polystyrine and crystal plate

	A	B	C	D	E	F	G	H	I
1	NADH ε340 =	6220	M-1cm-1
2
3		NADH			Buffer = 50mM phosphates, pH 7
4	Molar Mass (g/mol)	741.6
5	single component stock conc. (mM)	40			polystyrine plate info:	Falcon brand product # 35 1172
6	solubility	50 mg/mL in water, more if basic				"microtest Flat Bottom" polystyrene individually package. Not tissue culture treated plate, 96 well.
7	uL required	11,512.00			Order of operations:
8	surplus factor	2			1) Make buffer, read with pH probe. Says 7.06.
9	uL to shoot for	23024			2) Weigh 8.22 mg NADH & dissolve in 277.1 uL buffer. NADH bottle is from 2011.
10	mg to shoot for	682.984			3) Add required uL of NADH solution to each polystyrine well
11	mg actually weighed out	8.220			4) Add required uL of buffer to each polystyrine well
12	uL buffer to add	277.10			5) Read polystyrine plate for 10 min
13	check: M of solution	0.04			6) move solution to quartz plate and measure 10 min
14					7) set up for a mult-hour run in the crystal plate. Unfortunately the software crashed and the data was not available. In the morning, A340 was equal in all wells, indicating all of the NADH had degraded.
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17	row	col	uL NADH	uL buffer	total uL	true_mM_NADH	path_cm	theoretical_OD_from_known_mM
18	A	9	0	150	150	0.00	0.3825	0.00
19	A	10	0	150	150	0.00	0.3825	0.00
20	A	11	1	149	150	0.27	0.3825	0.63
21	A	12	1	149	150	0.27	0.3825	0.63
22	B	9	2	148	150	0.53	0.3825	1.27
23	B	10	2	148	150	0.53	0.3825	1.27
24	B	11	3	147	150	0.80	0.3825	1.90
25	B	12	3	147	150	0.80	0.3825	1.90
26	C	9	4	146	150	1.07	0.3825	2.54
27	C	10	4	146	150	1.07	0.3825	2.54
28	C	11	5	145	150	1.33	0.3825	3.17
29	C	12	5	145	150	1.33	0.3825	3.17
30	D	9	6	144	150	1.60	0.3825	3.81
31	D	10	6	144	150	1.60	0.3825	3.81
32	D	11	7	143	150	1.87	0.3825	4.44
33	D	12	7	143	150	1.87	0.3825	4.44
34	E	9	8	142	150	2.13	0.3825	5.08
35	E	10	8	142	150	2.13	0.3825	5.08
36	E	11	10	140	150	2.67	0.3825	6.34
37	E	12	10	140	150	2.67	0.3825	6.34
38	F	9	12	138	150	3.20	0.3825	7.61
39	F	10	12	138	150	3.20	0.3825	7.61
40	F	11	16	134	150	4.27	0.3825	10.15
41	F	12	16	134	150	4.27	0.3825	10.15
42	G	9	20	130	150	5.33	0.3825	12.69
43	G	10	20	130	150	5.33	0.3825	12.69
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45
46	RESULTS:
47		uL.NADH	uL.buffer	total.uL	true_mM_NADH	path_cm	theoretical_OD_from_known_mM	plate.material	mean.OD340
48	1	0	150	150	0	0.3825	0	polystyrene	0.174192105
49	2	1	149	150	0.27	0.3825	0.63	polystyrene	0.737472368
50	3	2	148	150	0.53	0.3825	1.27	polystyrene	1.252155263
51	4	3	147	150	0.8	0.3825	1.9	polystyrene	1.748663158
52	5	4	146	150	1.07	0.3825	2.54	polystyrene	2.308542105
53	6	5	145	150	1.33	0.3825	3.17	polystyrene	2.644335526
54	7	6	144	150	1.6	0.3825	3.81	polystyrene	2.932455263
55	8	7	143	150	1.87	0.3825	4.44	polystyrene	3.083059211
56	9	8	142	150	2.13	0.3825	5.08	polystyrene	3.194856579
57	10	10	140	150	2.67	0.3825	6.34	polystyrene	3.321723684
58	11	12	138	150	3.2	0.3825	7.61	polystyrene	3.432786842
59	12	16	134	150	4.27	0.3825	10.15	polystyrene	3.632619737
60	13	20	130	150	5.33	0.3825	12.69	polystyrene	3.647551316
61	14	0	150	150	0	0.3825	0	quartz	0.030160526
62	15	1	149	150	0.27	0.3825	0.63	quartz	0.482206579
63	16	2	148	150	0.53	0.3825	1.27	quartz	0.910155263
64	17	3	147	150	0.8	0.3825	1.9	quartz	1.301217105
65	18	4	146	150	1.07	0.3825	2.54	quartz	1.790414474
66	19	5	145	150	1.33	0.3825	3.17	quartz	2.123255263
67	20	6	144	150	1.6	0.3825	3.81	quartz	2.446368421
68	21	7	143	150	1.87	0.3825	4.44	quartz	2.699015789
69	22	8	142	150	2.13	0.3825	5.08	quartz	2.860985526
70	23	10	140	150	2.67	0.3825	6.34	quartz	3.078928947
71	24	12	138	150	3.2	0.3825	7.61	quartz	3.277535526
72	25	16	134	150	4.27	0.3825	10.15	quartz	3.531682895
73	26	20	130	150	5.33	0.3825	12.69	quartz	3.643409211
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