20150806_Jake_oly_mech_stress_cDNA_calcs
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1
Plate PositionSample ID
[DNased RNA] (ng/ul)
uL to 250ng RNA
uL dT primers (0.5ug/ug RNA) of a 1:10 dilution
H2O to 74.75uL
5x rxn buffer (uL)
10mM dNTPs (uL)
MMLV (uL)Master mix
Single Reaction (uL)
# Reactions
Total (uL)
2
A01DNased 42215 HM1 128.728.701.2564.802050.255x buffer2027540
3
B01DNased 42215 HM1 222.2411.241.2562.262050.25dNTPs527135
4
C01DNased 42215 HM1 325.149.941.2563.562050.25MMLV0.25276.75
5
D01DNased 42215 HM1 422.0511.341.2562.162050.25
6
E01DNased 42215 HM1 522.7810.971.2562.532050.25
7
F01DNased 42215 HM1 628.448.791.2564.712050.25
8
G01DNased 42215 HM1 728.268.851.2564.652050.25
9
H01DNased 42215 HM1 822.3811.171.2562.332050.25
10
A02DNased 42215 NM1 129.198.561.2564.942050.25
11
B02DNased 42215 NM1 226.539.421.2564.082050.25
12
C02DNased 42215 NM1 327.888.971.2564.532050.25
13
D02DNased 42215 NM1 429.718.411.2565.092050.25
14
E02DNased 42215 NM1 531.228.011.2565.492050.25
15
F02DNased 42215 NM1 630.898.091.2565.412050.25
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G02DNased 42215 NM1 724.4810.211.2563.292050.25
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H02DNased 42215 NM1 829.118.591.2564.912050.25
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A03DNased 42215 SM1 122.6111.061.2562.442050.25
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B03DNased 42215 SM1 229.968.341.2565.162050.25
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C03DNased 42215 SM1 327.019.261.2564.242050.25
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D03DNased 42215 SM1 427.29.191.2564.312050.25
22
E03DNased 42215 SM1 538.186.551.2566.952050.25
23
F03DNased 42215 SM1 628.038.921.2564.582050.25
24
G03DNased 42215 SM1 731.068.051.2565.452050.25
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H03DNased 42215 SM1 825.349.871.2563.632050.25
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20150806_Jake_oly_mech_stress_D