Equipment needed for Open Bio Labs
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Equipment PurposeKey:
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PCR Machine/ThermocyclerUsing controlled temperature cycling, for DNA amplification and other reactionsAbsolute Basics
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Pipettors P1000Tiny, precise liquid measurements. 100-1000ulFull-functioning Lab
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Centrifuge (SM- 1.5uL tubes)Spins tubes very fast to separate materials of different densities; ROTOR THAT HOLDS 1.5uL tubesCushy lab
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Centrifuge (LG- 15/50mL tubes)ROTOR THAT HOLDS 15-50mL tubes
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4°C Cold Storage (Refridgerator)
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-20°C Cold Storage (Freezer)CONSTANT TEMP/Manual Defrost (opposed to most modern freezers, have freeze/thaw cycles- very bad)
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Incubator (37°C)Growing cells
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Balance - Top Loader (g)
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Microwave ovenFOR LAB USE ONLY - Should be large enough to hold a 250 ml erlenmeyer flask.
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Pressure cookerSterilize glassware etc.
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Gel Electrophoresis boxDevice that uses electric current to separate DNA fragments based on size.
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Gel Electrophoresis power supplyPowers the box; runs a current with 100-200V or 30-200mA
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Micro Balance - Analytical (mg)More sensitive.
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UV or Blue Light BoxVisualize DNA stained with Ethiduim Bromide in electrophoresis gels (Most DIYbio groups have switched to SybrGreen, a non-hazardous stain that can be visualized in blue light or UV light.)
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-80°C Cold Storage (Freeeezer) / Liquid NitrogenFor long-term sample/cell storage
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Shaking Incubator (37°C)Growing liquid cultures, which needs aeration. OR just a shaker/rotator that can be placed inside an incubator
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Water bathHeat media to temp/incubate samples
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Heat block with magnetic spinnerMaintains Constant Temp, to heat experiments (common temps 42°C, 100°C)
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Fume Hood/Flamables Cabinet
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Spectrophotometer/AbsorbanceSpec: Measure bacterial population via density/tubitity at wavelength A600
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NanoDropMeasure DNA/Protein Conc. at A260/280
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Western Blot: Gel Box, Transfer boxFor visualizing proteins
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Rocker/End-over-end For gentle mixing, often overnight
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pH Meter
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Laser CutterMaking our own lab equipment
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3D Printer
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LyophilizerDessicating samples
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Vortex mixerQuickly mix liquids by vibrating rapidly
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Water purifierGenerates pure water for sensitive rxns
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Autoclavesteam sterilizer, used to make sterile media, sterilize objects -- VERY EXPENSIVE (try using a pressure cooker)
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MicroscopesMagnifyyy
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DNA sequencerSequencing strings of DNA
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DNA synthesizerSynthesizing strings of DNA
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qPCR machineQuantitative measurement of gene transcription
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Small Items:
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Erlenmeyer flasks250 mL
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Graduated Cylinders100 ml and 1000 ml size
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Beakers200 ml
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Storage Bottles500ml, 1000ml, screwcap, autoclave safe
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Magetic Stir Bars
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Thermometer
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Eye wash station
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Goggles
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Heat-resistance gloves
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Lab Coats
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First Aid Kit
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Spill Kits
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Fire extinguishers
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Test tube holders
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Pipette Manual PumpFor 1- 25 mL, much cheaper 'suction' device than pipettors
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1.5mL Tube Racks
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Sissors/tweasers
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Butane tourch (+ butane!)like they use to make Crème Brulee
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Freezer boxesto organize/store samples
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Spray bottles for disinfectant
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Glass/Sharps disposal box
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Continuous Supply/Disposibles:
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Ice/Dry Ice
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Deionized Water (DI H2O)or buy a Purifyer
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Biological Waste/Sharps Box
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Pipette TipsFor sizes .1-2ul, 2-200ul, and 100-1000ul
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Pipette Pump Tip/Serological For sizes 5ml, 10ml, 25ml
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1.5uL Microcentrifuge Tubes
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15ml Disposable Test TubesFalcon, SnapCap
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15ml, 40ml Disposible TubesScrewCap
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Perti Dishes/Plastic Plates
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Plastic Gloves
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Disinfectant (Ethanol)
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Disinfectant (Bleach)
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Benchpads
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Wipes/Paper Towels
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Kim wipes
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