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SAlleleDOB
# Fish
Owner
Mother genotypeFather genotypeNotesNotes
Swimming?
2
S1.1AB* x ABZ2 ix8.16.16~30RSGYes
3
S1.2AB* x ABZ2 ix8.16.16~40RSGYes
4
S3chfrDP1:Δ11 fish B & C ix10.4.162RSGOne pair left.  They didn't produce 25% progeny with swim bladder defectStl316 histology at 15-20 dpf, 1 pair left, waiting to count progeny at 8dpfNo
5
S6stl316/WIK hets -/-xWIK1.26.16~4RSGStl316 microCT and soft tissue brain scan- underwayYes
6
S7MZ stl316 -/-8.17.164RSGYes
7
S13rim2a ex4 crispr9.1.16~15RSGthese should be Oxed and genotyped for Rims2a mutationsYes
8
S14rim2a ex2 crispr9.1.166RSGthese should be Oxed and genotyped for Rims2a mutationsIn processYes
9
S17col2mGFP male x AB* female9.1.16~10RSGshould be outcrossed and screened for col2-gfp at some point, reestablish line (screen for col2GFP in jaw)ox'd 6/29/17Yes
10
S18tol2 col2>Cas9RSGIX and screen for green heart; keep the parents seperate until screening and euthanize those that do not give green heart.incrossed 5/2/17Yes
11
S22MZ lbx1a ix + lbx1b R1 crispr8.30.161RSGthese can be further Oxed to preferably to lbx1a mutant fish or hets or to AB*.outcross to lbx1b mutant female (+4), outcrossed to AB (progeny S43), but male diedNo
12
S23kif 6 sko/sko ix5.25.16~20RSGNeed to both Incross these and OX cross them preferably on the same day. We need to see more fish off for soft tissue brain scan at 30dpf.Outcrossed to AB (progeny S56)Yes
13
S24
kif 6 sko/sko + kif 6 ex2 talen + Loxp donor
6.7.16~25RSGthese can be Oxed to kif6 sko/sko mutant and if the clutch is good then depose of the donor fish.No
14
S25kif 6 sko/sko female x Foxjl GFP9.27.16~30RSGthese can be incrossed and screen for Foxj1 GFP to expand material for immunostaining for eppendymal cell markers.Incrossed multiple times.  Progeny screened for Foxj1 (S74)Yes
15
S29stl 297 het x mut ix9.29.16~100RSGplan sequencing for wt and mutantsYes
16
S31stl 300 -/WIK ix10.6.16~100RSGplan sequencing for wt and mutantsYes
17
S37adamts9 stl336 het x AB*
11.22.16
50RSGneed to genotype; ask Ryan about protocolGenotyped on 6.18.17. All remaining fish are hets.Yes
18
S39lbx1aECR1.1::GFP GFP+
10.21.16
25RSGix to reestablish lineYes
19
S40MZ stl 316 -/- ; H5tol2ats9FL inj F0
11.30.16
5RSGOX to stl316-/- and screen for green hearts. If not possible then cross to S44outcrossed to stl316-/- (S55) but there are only 4 fish and phenotype is severe so they might die, also outcrossed to S44 and got 70 embryos (4/26), will screen for green hearts next weekNo
20
S42adamts9 stl90 het x AB
11.29.16
10RSGGenotyped on 6.20.17, remaining fish are hets.Yes
21
S43MZ lbx1a + lbx1b R1 crispr x AB3.30.1713RSGuse to cross to lbx1b fishNo
22
S44
F0 tol2 H5 adamts9 in AB x stl 316/WIK het (+GFP hsants transgene +)
2.2.17~20RSGneed to genotype for stl316 mutation by sanger sequencingox to S40 4/26. Will screen for green hearts next week.No
23
S45MZ stl 316 -/- HETS3.1.17~15RSGYes
24
S46stl 63/+ gpr126 hets12.7.16~15RSGpair down to 10-12 fish 5 females 5 males. Need to genotype ask Ryan for protocolincrossed.  Progeny are S70(only kept homozygous mutants)Yes
25
S47F0 tol2 H5 adamts9 in AB*
11.29.16
12RSGINcross these fish and screen for green heart. Once you have these seperated we will gear up to test if the HS transgene is expressing and figure out the temporal expression lag after induction.No
26
S48chfr het ix "B & C pair"1.11.1711RSGfin clip keep hets , IX hets and screen for phenotype at 5 and 8 dpf.finclipped. kept hets. Crossed 3 pairs on 4/26. Waiting to count next weekNo
27
S49
kif 6 sko/sko; Foxj1GFP ix (screened for Foxj1)
3.2.17~6RSGYes
28
S50MZlbx1a hets3.8.171RSGfin clip and genotype.No
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S51MZlbx1a hets; ECR1 GFP (+)3.11.17~10RSGfin clip and genotype.cross with S54(done S71), genotype progeny for lbx1a, lbx1bYes
30
S52stl 97 -/- x AB*12.6.16~20RSGneed to IX to find carriersGot 3 crosses and parents are separated. Will look for kink in thorax at 15dpfNo
31
S53MZ stl 316 -/- x stl 316 het3.21.17~100RSGNo
32
S54lbx1b rank 1 crispr x AB*
11.22.16
3RSGMales were outcrossed to WT females. Eggs collected at 1dpf for genotypingNo
33
S55MZstl316-/-  HStol2GFP(+)4.6.171RSGneed to in cross and screen for green heart and begin assessing the HS transgene.Yes
34
S56kif6 sko hets4.7.174RSGneed to incross to make sure parents are transmitting the mutation .Yes
35
S57lbx1a ECR1 crispr F0 ix4.13.171RSGNeed to incross again at Johann's labNo
36
S58
lbx1b crisp F1 ix + 4female x +17, +8 male
4.18.17~70RSGNo
37
S59fez 2 ix4.13.17~50RSGYes
38
S60F0 tol2 H5ats9 in AB;stl316/WIK x MZstl316-/-ix + H5 tol2ats9 FL inj F04.26.17~40RSGScreened for green hearts.No
39
S61rim2a ex2 IX5.9.17~61TarikaGenotyped on 5.11.17.Yes
40
S62lbx1a ECR1 IX5.10.17~58RSGGenotyped 5.18.17 and bleached. Pair 1NO
41
S63kif6 sko/sko; FoxJ1 GFP ts x AB5.10.17~40RSGScreened for FoxJ1 on 5.15.17Yes
42
S64lbx1b crispr +17, +8 x AB4.25.17~55RSGNo
43
S65snx16 OX/AB4.13.17~50RSGNO
44
S66snx16 IX4.13.17~50RSGYes
45
S67F1 tol2 HS ats9 in AB; stl316/WIK IX (GFP+)6.15.17~60RGScreened for GFP in heartsNo
46
S68F0 (stl90 +/-) X (stl336 +/-)6.22.17~60RGYes
47
S69heatr5a CRISPR F0 IX5.25.172Yes
48
S70stl63 homozygous mutants 5.18.17~10RSGAll have been screened for "puffy ears"yes
49
S71MZ lbx1a+/-hets;ECRGFPxlbx1b crispr +17, +8 male #16.21.17~100RSGNo
50
S72ABs3.17.17~20RSGYes
51
S73fez2 ox 4.13.17~5RSGYes
52
S74kif6sko/+;Foxj1GFP ix 6.1.17~15RSGYes
53
S75ABs6.5.17~15RSGYes
54
S76AB*+Foxj1:kif6LGGFP F04.28.17~10RSGscreened for green eyesYes
55
S77kif6 sko/sko;Foxj1GFPix + Foxj1LGGFP F05.3.17~15RSGYes
56
S78kif sko/sko Fo IX7.11.17~60RGScreen for Green EyesS77 IXYes
57
S79AB + Foxj1; Kif6 GGFP Tol2 Fo IX7.14.17~50RGS76 IXYes
58
S80 stl 336 +/- IX6.29.17~40RGYes
59
S81stl 90 +/- IX6.29.17~80RGYes
60
S82stl 336 +/- IX7.20.17~3RGYes
61
S83stl 90 +/- IX8.8.17~6RGYes
62
S84stl 316 +/- IX6.30.17~30RGYes
63
S85stl 316 +/- IX6.30.17~15RGYes
64
S86stl 316 +/- IX8.8.17~50RGYes
65
S87stl90 +/- X stl336 +/-7.27.17~20RGYes
66
S88 stl 90 +/- IX6.29.17~30RGYes
67
S89kif 6; sko/sko F0 IX~40RGYes
68
S90stl 336 +/- IX6.29.17~27RGYes
69
S91stl 316 +/- IX8.17.17~100RGNo
70
S92stl 90 +/- IX8.18.17~30RGYes
71
S93stl 316 -/-  Mutants6.14.17~13RGYes
72
S94Acads_r3_ex5 CRISPR F07.7.17BRTInjected into S1.1 embryosNO
73
S95Appbp2_r2 CRISPR F07.7.17BRTInjected into S1.1 embryos
sacked 11.21.17 bc no mutant offspring
NO
74
S96Mllt10_r1 CRISPR F07.7.17BRTInjected into S1.1 embryosNO
75
S97lbx1b_r2_3stop CRISPR F07.26.17BRTNO
76
S98Tg[lbx1aECR1.1 TtoC]8.15.17BRT
Injected into S1.1 embryos, construct has been mutated to give the "protective" allele
77
S99lbx1a/+; lbx1b_r1; ECR1.1:GFP7.14.17BRTcross S43 to S51NO
78
S100ECR1delta Outcross8.16.17BRTS62 crossed to WT S1.2NO
79
S101AB ix 7.7.177NO
80
S102kif 6 sko/+; Foxj1GFP ix8.8.1710Yes
81
S103 AB ix 8.9.17Yes
82
S104kif 6 sko/+ ix8.17.17Yes
83
S105.1
stl 316 +/- ix "WTs"8.17.1730Stl 316 +/- hetstl316+/- hetYes
84
S105.2
stl 316 -/- 8.17.1720Stl 316 +/- hetstl316+/- het
85
S106kif 6 sko/+ het + tol2 foxj1::kif6GFP8.16.17Yes
86
S107 kif6 sko/+; Foxj1 GFP ix   Foxj1+8.17.17Yes
87
S108WIK8.22.17Yes
88
S109WIK8.22.17Yes
89
S110TU8.22.17Yes
90
S111TU8.22.17Yes
91
S112Fez2_ex4+3stopDonor F08.29.175BRTin S1.1 AB*NO
92
S113Fez2_ex4+3stopDonor F08.31.1760BRTin Q9 AB*NO
93
S114Heatr5a_r2+3stopDonor F08.29.175BRTin S1.1 AB*NO
94
S115Heatr5a_r2+3stopDonor F08.31.1760BRTin Q9 AB*NO
95
S116snx16_ex2+3stopDonor F08.29.175BRTin S1.1 AB*NO
96
S117snx16_ex2+3stopDonor F08.31.1760BRTin Q9 AB*NO
97
S118rims2a_ex4_CRISPR FO IX9/6/1740NSS13 rims2a_ex4_CRISPR FO S13 rims2a_ex4_CRISPR F0Yes
98
S119AB* IX9/6/1780RSG/RMS1S1 Yes
99
S120 Tol2 Ibx1a: ECR 1.1 TtoC GFP+ F09/6/17BRTNO
100
S121Tol2 Ibx1a: ECR 1.1 TtoC F0 (no GFP)9/6/17BRTNO