Gray Lab Fish Stock List

	A	B	D	E	F	G	H	I	J
1	S	Allele	DOB	# Fish	Owner	Mother genotype	Father genotype	Notes	Swimming?

2	S1.1	AB* x ABZ2 ix	8.16.16	~30	RSG				Yes
3	S1.2	AB* x ABZ2 ix	8.16.16	~40	RSG				Yes
4	S3	chfrDP1:Δ11 fish B & C ix	10.4.16	2	RSG			One pair left. They didn't produce 25% progeny with swim bladder defect	No
5	S6	stl316/WIK hets -/-xWIK	1.26.16	~4	RSG				Yes
6	S7	MZ stl316 -/-	8.17.16	4	RSG				Yes
7	S13	rim2a ex4 crispr	9.1.16	~15	RSG			these should be Oxed and genotyped for Rims2a mutations	Yes
8	S14	rim2a ex2 crispr	9.1.16	6	RSG			these should be Oxed and genotyped for Rims2a mutations	Yes
9	S17	col2mGFP male x AB* female	9.1.16	~10	RSG			should be outcrossed and screened for col2-gfp at some point, reestablish line (screen for col2GFP in jaw)	Yes
10	S18	tol2 col2>Cas9			RSG			IX and screen for green heart; keep the parents seperate until screening and euthanize those that do not give green heart.	Yes
11	S22	MZ lbx1a ix + lbx1b R1 crispr	8.30.16	1	RSG			these can be further Oxed to preferably to lbx1a mutant fish or hets or to AB*.	No
12	S23	kif 6 sko/sko ix	5.25.16	~20	RSG			Need to both Incross these and OX cross them preferably on the same day. We need to see more fish off for soft tissue brain scan at 30dpf.	Yes
13	S24	kif 6 sko/sko + kif 6 ex2 talen + Loxp donor	6.7.16	~25	RSG			these can be Oxed to kif6 sko/sko mutant and if the clutch is good then depose of the donor fish.	No
14	S25	kif 6 sko/sko female x Foxjl GFP	9.27.16	~30	RSG			these can be incrossed and screen for Foxj1 GFP to expand material for immunostaining for eppendymal cell markers.	Yes
15	S29	stl 297 het x mut ix	9.29.16	~100	RSG			plan sequencing for wt and mutants	Yes
16	S31	stl 300 -/WIK ix	10.6.16	~100	RSG			plan sequencing for wt and mutants	Yes
17	S37	adamts9 stl336 het x AB*	11.22.16	50	RSG			need to genotype; ask Ryan about protocol	Yes
18	S39	lbx1aECR1.1::GFP GFP+	10.21.16	25	RSG			ix to reestablish line	Yes
19	S40	MZ stl 316 -/- ; H5tol2ats9FL inj F0	11.30.16	5	RSG			OX to stl316-/- and screen for green hearts. If not possible then cross to S44	No
20	S42	adamts9 stl90 het x AB	11.29.16	10	RSG				Yes
21	S43	MZ lbx1a + lbx1b R1 crispr x AB	3.30.17	13	RSG			use to cross to lbx1b fish	No
22	S44	F0 tol2 H5 adamts9 in AB x stl 316/WIK het (+GFP hsants transgene +)	2.2.17	~20	RSG			need to genotype for stl316 mutation by sanger sequencing	No
23	S45	MZ stl 316 -/- HETS	3.1.17	~15	RSG				Yes
24	S46	stl 63/+ gpr126 hets	12.7.16	~15	RSG			pair down to 10-12 fish 5 females 5 males. Need to genotype ask Ryan for protocol	Yes
25	S47	F0 tol2 H5 adamts9 in AB*	11.29.16	12	RSG				No
26	S48	chfr het ix "B & C pair"	1.11.17	11	RSG			fin clip keep hets , IX hets and screen for phenotype at 5 and 8 dpf.	No
27	S49	kif 6 sko/sko; Foxj1GFP ix (screened for Foxj1)	3.2.17	~6	RSG				Yes
28	S50	MZlbx1a hets	3.8.17	1	RSG			fin clip and genotype.	No
29	S51	MZlbx1a hets; ECR1 GFP (+)	3.11.17	~10	RSG			fin clip and genotype.	Yes
30	S52	stl 97 -/- x AB*	12.6.16	~20	RSG			need to IX to find carriers	No
31	S53	MZ stl 316 -/- x stl 316 het	3.21.17	~100	RSG				No
32	S54	lbx1b rank 1 crispr x AB*	11.22.16	3	RSG			Males were outcrossed to WT females. Eggs collected at 1dpf for genotyping	No
33	S55	MZstl316-/- HStol2GFP(+)	4.6.17	1	RSG			need to in cross and screen for green heart and begin assessing the HS transgene.	Yes
34	S56	kif6 sko hets	4.7.17	4	RSG			need to incross to make sure parents are transmitting the mutation .	Yes
35	S57	lbx1a ECR1 crispr F0 ix	4.13.17	1	RSG			Need to incross again at Johann's lab	No
36	S58	lbx1b crisp F1 ix + 4female x +17, +8 male	4.18.17	~70	RSG				No
37	S59	fez 2 ix	4.13.17	~50	RSG				Yes
38	S60	F0 tol2 H5ats9 in AB;stl316/WIK x MZstl316-/-ix + H5 tol2ats9 FL inj F0	4.26.17	~40	RSG			Screened for green hearts.	No
39	S61	rim2a ex2 IX	5.9.17	~61	Tarika			Genotyped on 5.11.17.	Yes
40	S62	lbx1a ECR1 IX	5.10.17	~58	RSG			Genotyped 5.18.17 and bleached. Pair 1	NO
41	S63	kif6 sko/sko; FoxJ1 GFP ts x AB	5.10.17	~40	RSG			Screened for FoxJ1 on 5.15.17	Yes
42	S64	lbx1b crispr +17, +8 x AB	4.25.17	~55	RSG				No
43	S65	snx16 OX/AB	4.13.17	~50	RSG				NO
44	S66	snx16 IX	4.13.17	~50	RSG				Yes
45	S67	F1 tol2 HS ats9 in AB; stl316/WIK IX (GFP+)	6.15.17	~60	RG			Screened for GFP in hearts	No
46	S68	F0 (stl90 +/-) X (stl336 +/-)	6.22.17	~60	RG				Yes
47	S69	heatr5a CRISPR F0 IX	5.25.17	2					Yes
48	S70	stl63 homozygous mutants	5.18.17	~10	RSG				yes
49	S71	MZ lbx1a+/-hets;ECRGFPxlbx1b crispr +17, +8 male #1	6.21.17	~100	RSG				No
50	S72	ABs	3.17.17	~20	RSG				Yes
51	S73	fez2 ox	4.13.17	~5	RSG				Yes
52	S74	kif6sko/+;Foxj1GFP ix	6.1.17	~15	RSG				Yes
53	S75	ABs	6.5.17	~15	RSG				Yes
54	S76	AB*+Foxj1:kif6LGGFP F0	4.28.17	~10	RSG			screened for green eyes	Yes
55	S77	kif6 sko/sko;Foxj1GFPix + Foxj1LGGFP F0	5.3.17	~15	RSG				Yes
56	S78	kif sko/sko Fo IX	7.11.17	~60	RG			Screen for Green Eyes	Yes
57	S79	AB + Foxj1; Kif6 GGFP Tol2 Fo IX	7.14.17	~50	RG				Yes
58	S80	stl 336 +/- IX	6.29.17	~40	RG				Yes
59	S81	stl 90 +/- IX	6.29.17	~80	RG				Yes
60	S82	stl 336 +/- IX	7.20.17	~3	RG				Yes
61	S83	stl 90 +/- IX	8.8.17	~6	RG				Yes
62	S84	stl 316 +/- IX	6.30.17	~30	RG				Yes
63	S85	stl 316 +/- IX	6.30.17	~15	RG				Yes
64	S86	stl 316 +/- IX	8.8.17	~50	RG				Yes
65	S87	stl90 +/- X stl336 +/-	7.27.17	~20	RG				Yes
66	S88	stl 90 +/- IX	6.29.17	~30	RG				Yes
67	S89	kif 6; sko/sko F0 IX		~40	RG				Yes
68	S90	stl 336 +/- IX	6.29.17	~27	RG				Yes
69	S91	stl 316 +/- IX	8.17.17	~100	RG				No
70	S92	stl 90 +/- IX	8.18.17	~30	RG				Yes
71	S93	stl 316 -/- Mutants	6.14.17	~13	RG				Yes
72	S94	Acads_r3_ex5 CRISPR F0	7.7.17		BRT			Injected into S1.1 embryos	NO
73	S95	Appbp2_r2 CRISPR F0	7.7.17		BRT			Injected into S1.1 embryos	NO
74	S96	Mllt10_r1 CRISPR F0	7.7.17		BRT			Injected into S1.1 embryos	NO
75	S97	lbx1b_r2_3stop CRISPR F0	7.26.17		BRT				NO
76	S98	Tg[lbx1aECR1.1 TtoC]	8.15.17		BRT			Injected into S1.1 embryos, construct has been mutated to give the "protective" allele
77	S99	lbx1a/+; lbx1b_r1; ECR1.1:GFP	7.14.17		BRT			cross S43 to S51	NO
78	S100	ECR1delta Outcross	8.16.17		BRT			S62 crossed to WT S1.2	NO
79	S101	AB ix	7.7.17	7					NO
80	S102	kif 6 sko/+; Foxj1GFP ix	8.8.17	10					Yes
81	S103	AB ix	8.9.17						Yes
82	S104	kif 6 sko/+ ix	8.17.17						Yes
83	S105.1	stl 316 +/- ix "WTs"	8.17.17	30		Stl 316 +/- het	stl316+/- het		Yes
84	S105.2	stl 316 -/-	8.17.17	20		Stl 316 +/- het	stl316+/- het
85	S106	kif 6 sko/+ het + tol2 foxj1::kif6GFP	8.16.17						Yes
86	S107	kif6 sko/+; Foxj1 GFP ix Foxj1+	8.17.17						Yes
87	S108	WIK	8.22.17						Yes
88	S109	WIK	8.22.17						Yes
89	S110	TU	8.22.17						Yes
90	S111	TU	8.22.17						Yes
91	S112	Fez2_ex4+3stopDonor F0	8.29.17	5	BRT			in S1.1 AB*	NO
92	S113	Fez2_ex4+3stopDonor F0	8.31.17	60	BRT			in Q9 AB*	NO
93	S114	Heatr5a_r2+3stopDonor F0	8.29.17	5	BRT			in S1.1 AB*	NO
94	S115	Heatr5a_r2+3stopDonor F0	8.31.17	60	BRT			in Q9 AB*	NO
95	S116	snx16_ex2+3stopDonor F0	8.29.17	5	BRT			in S1.1 AB*	NO
96	S117	snx16_ex2+3stopDonor F0	8.31.17	60	BRT			in Q9 AB*	NO
97	S118	rims2a_ex4_CRISPR FO IX	9/6/17	40	NS	S13 rims2a_ex4_CRISPR FO	S13 rims2a_ex4_CRISPR F0		Yes
98	S119	AB* IX	9/6/17	80	RSG/RM	S1	S1		Yes
99	S120	Tol2 Ibx1a: ECR 1.1 TtoC GFP+ F0	9/6/17		BRT				NO
100	S121	Tol2 Ibx1a: ECR 1.1 TtoC F0 (no GFP)	9/6/17		BRT				NO