Triage-Summer Fall'18 Targets

	A	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB	AC	AD	AE	AF	AG	AH	AI	AJ	AK	AL	AM	AN	AO	AP	AQ	AR	AS
1	Index	Target	Organism	Researcher On Target	Reviewer 1 Add your name here!	Reviewer 2	Reviewer 1 Rating (Good, Bad, OK)	Reviewer 2 Rating (Good, Bad, OK)	Notes A- Add notes here!	Notes B	Notes C	Researcher(s) on target	Status	Overlap Primer Designers	Tail primers Designer	Prefix IDT Ordering (yellow = ordered)	Forward Tail Primer in 5' to 3' orientation	Reverse Tail Primer in 5' to 3' orientation	Disease/Relevance	NOTES D	Type (anhydrase, reductase, phosphatase, etc.)	E.C.	structure?	homolog structure? % coverage on % identities	transmembrane region?	Complex?	activity (from Brenda site)	assay	SOURCE / PI	Essentiality	Druggability	TDR Targets Link or EuPath, etc	VDS Target page	Gene (NOT gene ontology)	Amino Acid Sequence	substrate available?	supplier	Second Substrate	# of inhibitors	assay	known inhibitors	Notes E	Notes F	Notes D (a.a. size)

2	1	Lactate Dehydrogenase	Cryptosporidium parvum		Inaara S.		Good		Crystal Structure: http://www.rcsb.org/structure/2FN7	https://www.ncbi.nlm.nih.gov/pubmed/25542170	https://www.sciencedirect.com/science/article/pii/S0020751917301662					CpLDH			Cryptosporidium infection		Dehydrogenase	1.1.1.27	2FN7	4ND4	1 predicted	no	(S)-lactate + NAD+ = pyruvate + NADH + H+	Lactate Dehydrogenase Activity Assay Kit	n/a	Essential as a regulaor of glycolosis inhibition of this enzyme causes the loss of energy production.	Druggable becayse Gossypol does competitive inhibition with NADH.		TargetSp18 - Lactate dehydogenase (//Cryptosporidium parvum//)..............................................................................................................................................Jacky X.	Tm-LDHA		(S)-lactate	sigma aldrich	NAD+	1	https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak066bul.pdf	Gossypol			321
3	2	Lactate Dehydrogenase	P. falciparum		Eduardo C.		Good		Lactate dehydrogenase is present in this. PDB structure is available as well (http://www.rcsb.org/structure/2X8L). Causes malaria (very serious)	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122489/	https://www.cdc.gov/malaria/about/biology/parasites.html			Eduardo C.	Eduardo C.	PfLDH	TACTTCCAATCCATGATGGCGCCGAAAGCG	GTC ATT TCC ACC TAT TTA CGC GAG TGC TTT CAT ACG TTT GG	Malaria		Dehydrogenase	1.1.1.27	2X8L	4ND4	1 predicted	No	(S)-lactate + NAD+ = pyruvate + NADH + H+	Lactate Dehydrogenase (LDH) Activity Assay Kit	Chan, Kit-Lam	Essential enzyme that regenerates nicotinamide adenine dinucleotide (NAD+) from NADH. Essential for growth.	0.8	http://tdrtargets.org/targets/view?gene_id=2126	N/A	PF3D7_1324900 ID: 814112		(S)-lactate	Sigma-Aldrich	NAD+	1	https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak066bul.pdf	Gossypol
4	3	6-phosphogluconate dehydrogenase, decarboxylating, putative	Leishmania major						needs oligos (Dr. B)	homology model						Lm6PGDH			Leishmaniasis is a vector-borne disease that is transmitted by sandflies and caused by obligate intracellular protozoa of the genus Leishmania.		dehydrogenase	1.1.1.44	no	T. brucei (PDB: 1PGJ) 73% identity, 97 % query	no		6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+	Continuous spectrophotmetric Rate Determination	Steven, Mayur	The enzyme participates in the oxidative branch of the pentose phosphate pathway, whose main purpose is to produce NADPH and pentose for biosynthetic reactions. Highly specific for NADP+. cf. EC 1.1.1.343, phosphogluconate dehydrogenase (NAD+-dependent, decarboxylating).	0.8	http://tdrtargets.org/targets/view?gene_id=23670		LmjF35.3340	MSNDLGIIGLGVMGANLALNIAEKGFKVAVFNRTYAKTTSFLKEHESEKFAANLNGYETMKEFAASLKKPRRAFILVQAG AATDSTIEQLKEVFENGDIIIDTGNANFKDQDKRAAQLESQGLRFLGMGISGGEEGARKGPAFFPGGTPSVWEEVRPIVE AAAAKAEDGRPCVTFNGKGGAGSCVKMYHNAGEYAVLQIWGEAYSALLAFGFDNDQIADVFESWKADGFLKSYMLDISIA ACRAREATGNYLSEKVKDRIGSKGTGLWSAQEALEIGVPAPSLNMAVISRQMTMYKGERIANCKAFPNFPRGPSEEATDK SPNSPEAKKLYHAVSLCIIASYAQMFQCLRELDKVYGFGLNLPATIATFRAGCILQGYLLGPMTKAFEENPNLPNLMDAF TKEIAAGLDDCRQILAKLTVNTAVSLPVMMASLSYINAMYTETLPYGQLVSLQRDVFGRHGYERTDKDGRESFEWPALQ	6 Phosphogluconate ($86.30)	Sigma Aldrich	Beta-Nicotinamide Adenine Dinucleotide, Oxidized form ($89.80)	tons	Continuous spectrophotmetric Rate Determination	http://www.brenda-enzymes.org/enzyme.php?ecno=1.1.1.44	assay methodology: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/6phosphoglucdehydrog75.pdf		479
5	4	6-phosphogluconate dehydrogenase, decarboxylating, putative	T. cruzi		Pareesa H.					homology model		Tyler G.				Tc6pgdh	TACTTCCAATCCATGGACGTTGGCATCGTTGGTC	TATCCACCTTTACTGTTATTGCAGCTCCGGCCACTG	Chagas Disease		dehydrogenase	1.1.1.44	No	T. brucei (PDB: 1PGJ)	No	Dimer	6-phosphono-D-gluconate + NADP+=D-ribulose 5-phosphate + NADPH	Continuous Spectrophotometric Rate Determination at 340nm	Bella, Josue	Involved in the bacterial pentose phosphate pathway	0.6	http://tdrtargets.org/targets/view?gene_id=49268	Not Avaliable	Tc00.1047053510663.70 9 (from Gene DB)		6-Phosphogluconic acid trisodium salt (P7877) $86.30	Sigma Aldrich	β-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate (N0505) $89.80	alot	Continuous spectrophotmetric Rate Determination	http://www.brenda-enzymes.org/enzyme.php?ecno=1.1.1.44&Suchword=&organism%5B%5D=Trypanosoma+cruzi			477
6	5	D-alanine:D-alanine ligase	Staphylococcus aureus		Anthony Z.				Enzyme is essential to the formation of bacterial cell walls and can result in resistance to vanomycin	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1622796/						SaDAL	TACTTCCAATCCATGACCAAAGAGAACATCTGCAT	TATCCACCTTTACTGTTAGTCGATCTTATACTTGTTTTTCTGCTTG	Skin infections, food poisoning, hospital-acquired infections	Can be commensal but can cause life-threatening diseases	Ligase	6.3.2.4	2I80	N/A	N/A	Biological unit = dimer	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine	colorimetric assay for high-throughput inhibitor screening	Liu, S	Required for final step in the synthesis of peptidoglycan (bacterial cell walls)	Yes, has been a target before and has been subject to evolution of antibiotic resistance		N/A	http://www.genedb.org/web-artemis/?src=scaffold105.1_size92539.2.8344-69665.final&base=49455&bases=3182		Yes, D-Ala-D-Ala	Sigma Aldrich	ATP	Many	colorimetric assay for high-throughput inhibitor screening	phosphinic acid dipeptide analogues, Diazenedicarboxamides			345
7	6	D-alanine:D-alanine ligase	Bacillus anthracis		Moses											BaDADA	TACTTCCAATCCATGGCGAACGACTACCTGTTCAC	TATCCACCTTTACTGTTAAGCAACTTGTACCGCCGG	Anthrax	Affects most livestock and humans	Ligase	6.3.2.4	Yes. 3R5X				ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine	Combined assay (in vitro method)		Required for peptidoglycan layer in cell walls of bacterial species	Yes Ddl can be a target		n/a	ddl (ddlB) 10.2210/pdb3R5X/pdb		Yes; D-alanyl-D-alanine	Sigma Aldrich	ATP	Tons	Combined assay	http://www.brenda-enzymes.org/enzyme.php?ecno=6.3.2.4#NATURAL%20SUBSTRATES			307
8	7	D-alanine:D-alanine ligase	Yersinia pestis													YpDala					Ligase	6.3.2.4					ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine	Combined assay (in vitro method)		Required for peptidoglycan layer in cell walls of bacterial species	Yes Ddl can be a target		n/a			Yes; D-alanyl-D-alanine	Sigma Aldrich	ATP	Tons	Combined assay	http://www.brenda-enzymes.org/enzyme.php?ecno=6.3.2.4#NATURAL%20SUBSTRATES
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