Bradford Assay - 1 - Google Sheets

	B	C	D	E	F	G	H	J
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2	Bradford Reagent
3		Stok		2.00	x	10.00	ml	https://zageno.com/l/ts-bradford-assay
4	CBB G250 / H2O	1.00	% (w/v)	0.02	% (w/v)	0.20	ml
5	Etanol	100.00	% (v/v)	9.40	% (v/v)	0.94	ml
6	Fosforik Asit	85.00	% (v/v)	17.00	% (v/v)	2.00	ml
7	H2O					6.86	ml
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9	Kuyucuklarda:
10		Stok		Çalışan		150	µl
11	Bradford Reagent	2.00	x	1	x	75	µl
12	Std (ya da örnek)	1	µg/µl	X	µg	Y	µl
13	H2O (ya da tampon)					Z	µl
14
15	Standartlar için:
16		X (BSA) (µg)	Y (µl)	Z (H2O) (µl)
17		1	1	74
18		2.5	2.5	72.5
19		5	5	70
20		7.5	7.5	67.5
21		10	10	65
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23	Örnekler için:
24		X (?)	Y (µl)	Z (H2O) (µl)
25			5	70
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32	Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248-54. doi: 10.1006/abio.1976.9999. PMID: 942051.
33	Kielkopf CL, Bauer W, Urbatsch IL. Bradford Assay for Determining Protein Concentration. Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102269. doi: 10.1101/pdb.prot102269. PMID: 32238597.
34	Ernst O, Zor T. Linearization of the bradford protein assay. J Vis Exp. 2010 Apr 12;(38):1918. doi: 10.3791/1918. PMID: 20386536; PMCID: PMC3164080.
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